Two distinct interstitial macrophage populations coexist across tissues in specific subtissular niches

[en] Macrophages are a heterogeneous cell population involved in tissue homeostasis, inflammation, and various pathologies. Although the major tissue-resident macrophage populations have been extensively studied, interstitial macrophages (IMs) residing within the tissue parenchyma remain poorly defined. Here we studied IMs from murine lung, fat, heart, and dermis. We identified two independent IM subpopulations that are conserved across tissues: Lyve1 lo MHCII hi CX3CR1 hi (Lyve1 lo MHCII hi ) and Lyve1 hi MHCII lo CX3CR1 lo (Lyve1 hi MHCII lo ) monocyte-derived IMs, with distinct gene expression profiles, phenotypes, functions, and localizations. Using a new mouse model of inducible macrophage depletion (Slco2b1 flox/DTR ), we found that the absence of Lyve1 hi MHCII lo IMs exacerbated experimental lung fibrosis. Thus, we demonstrate that two independent populations of IMs coexist across tissues and exhibit conserved niche-dependent functional programming. 2017 © The Authors.

Disciplines :

Life sciences: Multidisciplinary, general & others

Author, co-author :

Chakarov, S.; Singapore Immunology Network (SIgN), A*STAR, 8A Biomedical Grove, Immunos Building, Level 3, Singapore, 138648, Singapore

Lim, H. Y.; Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 119228, Singapore

Tan, L.; Singapore Immunology Network (SIgN), A*STAR, 8A Biomedical Grove, Immunos Building, Level 3, Singapore, 138648, Singapore

Lim, S. Y.; Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 119228, Singapore

See, P.; Singapore Immunology Network (SIgN), A*STAR, 8A Biomedical Grove, Immunos Building, Level 3, Singapore, 138648, Singapore

Lum, J.; Singapore Immunology Network (SIgN), A*STAR, 8A Biomedical Grove, Immunos Building, Level 3, Singapore, 138648, Singapore

Zhang, X.-M.; Singapore Immunology Network (SIgN), A*STAR, 8A Biomedical Grove, Immunos Building, Level 3, Singapore, 138648, Singapore

Foo, S.; Singapore Immunology Network (SIgN), A*STAR, 8A Biomedical Grove, Immunos Building, Level 3, Singapore, 138648, Singapore

Nakamizo, S.; Singapore Immunology Network (SIgN), A*STAR, 8A Biomedical Grove, Immunos Building, Level 3, Singapore, 138648, Singapore

Duan, K.; Singapore Immunology Network (SIgN), A*STAR, 8A Biomedical Grove, Immunos Building, Level 3, Singapore, 138648, Singapore

More authors (21 more)

Language :

English

Title :

Two distinct interstitial macrophage populations coexist across tissues in specific subtissular niches

Publication date :

2019

Journal title :

Science

ISSN :

0036-8075

eISSN :

1095-9203

Publisher :

American Association for the Advancement of Science

Volume :

363

Issue :

6432

Peer reviewed :

Peer Reviewed verified by ORBi

European Projects :

H2020 - 801823 - IM-ID - Defining the intrinsic transcriptional programs and the microenvironmental signals tailoring lung Interstitial Macrophage IDentity

Funders :

CE - Commission Européenne

Available on ORBi :

since 08 April 2019

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