Cuticular hydrocarbon composition does not allow Harmonia axyridis males to identify the mating status of sexual partners

Legrand, Pauline; Vanderplanck, Maryse; Lorge, Stéphanie; Maesen, Philippe; Lognay, Georges; Vilcinskas, Andreas; Vogel, Heiko; Estoup, Arnaud; Foucaud, Julien; Francis, Frédéric; Facon, Benoit; Verheggen, François

doi:10.1127/entomologia/2019/0552

Article (Scientific journals)

Cuticular hydrocarbon composition does not allow Harmonia axyridis males to identify the mating status of sexual partners

Legrand, Pauline; Vanderplanck, Maryse; Lorge, Stéphanie et al.

2019 • In Entomologia Generalis, 38 (3), p. 211 - 224

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/234016

DOI
10.1127/entomologia/2019/0552

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Abstract :

[en] Males of polyandrous species have to overcome sperm competition. They should select their mate based on the reproductive status of the female to increase their own fitness. Because the sexual behavior of lady beetles relies on semiochemicals, with cuticular hydrocarbons (CHCs) being used for mate recognition, we developed and tested two hypotheses. First, we hypothesized that the cuticular hydrocarbon profile qualitatively and quantitatively differs between virgin and mated Harmonia axyridis females, regardless of the color morph. Second, we hypothesized that males discriminate virgin and mated females, preferring copulating with virgin females, rather than previously mated ones, to avoid sperm competition and subsequently increase their fitness. CHCs were solvent-extracted before being quantified and identified by gas chromatography. We found no qualitative differences between mated and unmated females irrespective of the morph; however, quantitative differences were detected. Specifically, the CHC profiles of mated females presented higher concentrations of alkenes, including 9-pentacosene, 9-heptacosene, and 9-hentriacontene. During dual-choice behavioral assays, males equally copulated with virgin and mated females. Our results suggest that there is no CHC-based discrimination strategy in virgin males of H. axyridis between virgin and once-mated females. We discuss alternative strategies that might be used in this lady beetle species.

Disciplines :

Entomology & pest control

Author, co-author :

Legrand, Pauline ^✱

Vanderplanck, Maryse ^✱; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Analyse, qual. et risques - Labo. de Chimie analytique

Lorge, Stéphanie ; Université de Liège - ULiège > Bureau Environnement et Analyse (GxABT)

Maesen, Philippe ; Université de Liège - ULiège > Dvlpt. méth. chimie app. à l'agri., l'agro-ali. et/ou l'env.

Lognay, Georges ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie des agro-biosystèmes

Vilcinskas, Andreas

Vogel, Heiko

Estoup, Arnaud

Foucaud, Julien

Francis, Frédéric ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Gestion durable des bio-agresseurs

Facon, Benoit

Verheggen, François ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Gestion durable des bio-agresseurs

^✱ These authors have contributed equally to this work.

Language :

English

Title :

Cuticular hydrocarbon composition does not allow Harmonia axyridis males to identify the mating status of sexual partners

Publication date :

2019

Journal title :

Entomologia Generalis

ISSN :

0171-8177

eISSN :

2363-7102

Publisher :

E. Schweizerbart'sche Verlagsbuchhandlung, Germany

Volume :

Issue :

Pages :

211 - 224

Peer reviewed :

Peer Reviewed verified by ORBi

European Projects :

H2020 - 642420 - BiodivERsA3 - Consolidating the European Research Area on biodiversity and ecosystem services

Funders :

CE - Commission Européenne

Available on ORBi :

since 27 March 2019

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