Reference : Highly sensitive and selective separation of intact parathyroid hormone and variants ...
Scientific journals : Article
Human health sciences : Pharmacy, pharmacology & toxicology
http://hdl.handle.net/2268/233706
Highly sensitive and selective separation of intact parathyroid hormone and variants by sheathless CE-ESI-MS/MS.
English
Nyssen, Laurent mailto [Université de Liège - ULiège > Département de pharmacie > Chimie médicale >]
Fillet, Marianne mailto [Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments >]
Cavalier, Etienne mailto [Université de Liège - ULiège > Département de pharmacie > Chimie médicale >]
Servais, Anne-Catherine mailto [Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments >]
2019
Electrophoresis
Yes (verified by ORBi)
International
01730835
15222683
Germany
[en] CE-ESI-MS/MS ; PTH ; native protein ; sheathless interface ; variants
[en] Parathyroid hormone (PTH) is a common clinical marker whose quantification relies on immunoassays, giving variable results as batch, brand, or target epitope changes. Sheathless CE-ESI-MS, combining CE resolution power and low-flow ESI sensitivity, was applied to the analysis of PTH in its native conformation in the presence of related forms. Fused silica and neutral-coated capillaries were investigated, as well as preconcentration methods such as transient isotachophoresis (t-ITP), field-amplified sample injection (FASI) and electrokinetic supercharging (EKS). The method for the separation of PTH and its variants was first developed using fused-silica capillary with UV detection. An acidic background electrolyte (BGE) was used to separate 1-84 PTH (full length), 7-84 PTH and 1-34 PTH. Acetonitrile was added to the BGE to reduce peptide adsorption onto the capillary wall and t-ITP was used as analyte preconcentration method. The method was then transferred to a sheathless CE-ESI-MS instrument. When using a fused silica capillary, CE-MS was limited to mug/mL levels. The use of a neutral coating combined with FASI or EKS allowed a significant increase in sensitivity. Under these conditions, 1-84 PTH, 7-84 PTH and 1-34 PTH were detected at concentrations in the low ng/mL (FASI) or pg/mL (EKS) range. This article is protected by copyright. All rights reserved.
Centre Interdisciplinaire de Recherche sur le Médicament - CIRM
http://hdl.handle.net/2268/233706
10.1002/elps.201800507
This article is protected by copyright. All rights reserved.

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