Article (Scientific journals)
Anti-biofouling activity of Ranaspumin-2 bio-surfactant immobilized on catechol-functional PMMA thin layers prepared by atmospheric plasma deposition
Czuba, Urszula; Quintana, Robert; Lassaux, Patricia et al.
2019In Colloids and Surfaces B: Biointerfaces, 178, p. 120-128
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Keywords :
coating
Abstract :
[en] The deposition of polymeric thin layers bearing reactive functional groups is a promising solution to provide functionality on otherwise inert surfaces, for instance, for bioconjugation purposes. Atmospheric pressure plasma (AP plasma) deposition technology offers many advantages, such as fast deposition rates, low costs, low waste generation and suitability for coating various kind of material surfaces. In this work, the AP plasma-assisted copolymerization of methyl methacrylate (MMA) with a vinyl derivative of L-DOPA was studied in order to deposit coatings with reactive catechol/quinone groups suitable for protein covalent immobilization. The effect of adding a chemical cross-linker, between 0 and 2 mol%, to the monomer mixture is also studied in order to prepare robust plasma PMMA-based layers in liquid physiological media. The layer prepared with 0.2 mol% of cross-linker shows the best balance between stability in saline-buffered media and surface functionalization. Bioconjugation via the grafting of Ranaspumin-2 recombinant, a naturally occurring surfactant protein, is carried out in a single step after plasma deposition. Protein immobilization is corroborated by Quartz Crystal Microbalance with Dissipation (QCM-D) and Surface Plasmon Resonance (SPR) analyses and confirmed via Epicocconone staining, X-Ray Photoemission Spectroscopy (XPS) and Time of Flight Secondary Ion Mass Spectrometry (ToF-SIMS) measurements and surface wettability characterizations. The bio-functionalized layers presented an enhanced activity against the adhesion of Human Serum Albumin (HSA), indicating the grafting potential of the Ranaspumin-2 bio-surfactant to produce anti-biofouling functional coatings.
Research center :
CESAM - Complex and Entangled Systems from Atoms to Materials - ULiège
Center for Education and Research on Macromolecules (CERM), Belgium
Disciplines :
Chemistry
Materials science & engineering
Author, co-author :
Czuba, Urszula;  Institute of Science and Technology (LIST), Materials Research and Technology Department, Luxembourg > University of Liège (ULiège), Complex and Entangled Systems from Atoms to Materials (CESAM), Center for Education and Research on Macromolecules (CERM), Belgium
Quintana, Robert;  Institute of Science and Technology (LIST), Materials Research and Technology Department, Luxembourg
Lassaux, Patricia ;  University of Liège (ULiège), GIGA-R, Molecular Biomimetic and Protein Engineering Laboratory (MBPEL), Belgium
Bombera, Radoslaw;  European Commission, Joint Research Centre (JRC), Ispra, Italy
Ceccone, Giacomo;  European Commission, Joint Research Centre (JRC), Ispra, Italy
Bañuls-Ciscar, Jorge;  European Commission, Joint Research Centre (JRC), Ispra, Italy
Moreno-Couranjou, Maryline;  Institute of Science and Technology (LIST), Materials Research and Technology Department, Luxembourg
Detrembleur, Christophe ;  University of Liège (ULiège), Complex and Entangled Systems from Atoms to Materials (CESAM), Center for Education and Research on Macromolecules (CERM), Belgium
Choquet, Patrick;  Institute of Science and Technology (LIST), Materials Research and Technology Department, Luxembourg
Language :
English
Title :
Anti-biofouling activity of Ranaspumin-2 bio-surfactant immobilized on catechol-functional PMMA thin layers prepared by atmospheric plasma deposition
Publication date :
01 June 2019
Journal title :
Colloids and Surfaces B: Biointerfaces
ISSN :
0927-7765
Publisher :
Elsevier, Netherlands
Volume :
178
Pages :
120-128
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
FNR - Fonds National de la Recherche [LU]
Région wallonne [BE]
Available on ORBi :
since 08 March 2019

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