CRF19_cpx is an Evolutionary fit HIV-1 Variant Strongly Associated With Rapid Progression to AIDS in Cuba

CRF19; Cuba; HIV-1; Progression to AIDS; Variant; CXCL9 chemokine; Fas ligand; RANTES; Pol protein; Article; CD4 lymphocyte count; Human immunodeficiency virus 1 infection; Acquired Immunodeficiency Syndrome; HIV Infections; Human immunodeficiency virus 1; Adult; Biological Evolution; Coinfection; Drug Resistance, Viral; Female; Genetic Variation; Humans; Male; Retrospective Studies; Sexual Behavior; Viral Load; Young Adult

Abstract :

[en] Background: Clinicians reported an increasing trend of rapid progression (RP) (AIDS within 3. years of infection) in Cuba. Methods: Recently infected patients were prospectively sampled, 52 RP at AIDS diagnosis (AIDS-RP) and 21 without AIDS in the same time frame (non-AIDS). 22 patients were sampled at AIDS diagnosis (chronic-AIDS) retrospectively assessed as >. 3. years infected. Clinical, demographic, virological, epidemiological and immunological data were collected. Pol and env sequences were used for subtyping, transmission cluster analysis, and prediction of resistance, co-receptor use and evolutionary fitness. Host, immunological and viral predictors of RP were explored through data mining. Findings: Subtyping revealed 26 subtype B strains, 6 C, 6 CRF18_cpx, 9 CRF19_cpx, 29 BG-recombinants and other subtypes/URFs. All patients infected with CRF19 belonged to the AIDS-RP group. Data mining identified CRF19, oral candidiasis and RANTES levels as the strongest predictors of AIDS-RP. CRF19 was more frequently predicted to use the CXCR4 co-receptor, had higher fitness scores in the protease region, and patients had higher viral load at diagnosis. Interpretation: CRF19 is a recombinant of subtype D (C-part of Gag, PR, RT and nef), subtype A (N-part of Gag, Integrase, Env) and subtype G (Vif, Vpr, Vpu and C-part of Env). Since subtypes D and A have been associated with respectively faster and slower disease progression, our findings might indicate a fit PR driving high viral load, which in combination with co-infections may boost RANTES levels and thus CXCR4 use, potentially explaining the fast progression. We propose that CRF19 is evolutionary very fit and causing rapid progression to AIDS in many newly infected patients in Cuba. © 2015.

Disciplines :

Immunology & infectious disease

Author, co-author :

Kouri, V.; Virology Department, Institute of Tropical Medicine Pedro Kourí, Autopista Novia del Mediodía, Marianao 13, Havana City, Cuba

Khouri, R.; KU Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Clinical and Epidemiological Virology, Leuven, Belgium, LIMI-LIP, Centro de Pesquisa Gonçalo Moniz, FIOCRUZ, Salvador-Bahia, Brazil

Alemán, Y.; Virology Department, Institute of Tropical Medicine Pedro Kourí, Autopista Novia del Mediodía, Marianao 13, Havana City, Cuba

Abrahantes, Y.; Virology Department, Institute of Tropical Medicine Pedro Kourí, Autopista Novia del Mediodía, Marianao 13, Havana City, Cuba

Vercauteren, J.; KU Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Clinical and Epidemiological Virology, Leuven, Belgium

Pineda-Peña, A.-C.; KU Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Clinical and Epidemiological Virology, Leuven, Belgium, Clinical and Molecular Infectious Diseases Group, Faculty of Sciences and Mathematics, Universidad del Rosario, Bogotá, Colombia

Theys, K.; KU Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Clinical and Epidemiological Virology, Leuven, Belgium

Megens, S.; KU Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Clinical and Epidemiological Virology, Leuven, Belgium

Moutschen, Michel ; Université de Liège - ULiège > Département des sciences cliniques > GIGA-R:Immunopath. - Maladies infect. et médec. inter. gén.

Pfeifer, N.; Department of Computational Biology and Applied Algorithmics, Max Planck Institute for Informatics, Campus E1 4, Saarbrücken, Germany

More authors (6 more)

Language :

English

Title :

CRF19_cpx is an Evolutionary fit HIV-1 Variant Strongly Associated With Rapid Progression to AIDS in Cuba

Publication date :

2015

Journal title :

EBioMedicine

eISSN :

2352-3964

Publisher :

Elsevier

Volume :

Issue :

Pages :

244-254

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 04 March 2019

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