Article (Scientific journals)
In vitro maturation affects chromosome segregation, spindle morphology and acetylation of lysine 16 on histone H4 in horse oocytes
Franciosi, Federica; Goudet, Ghylene; Tessaro, Irene et al.
2017In Reproduction, Fertility and Development, 29 (4), p. 721-730
Peer Reviewed verified by ORBi
 

Files


Full Text
article_Franciosi_RFD_2017.pdf
Publisher postprint (679.7 kB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Article; RNA purification
Abstract :
[en] Implantation failure and genetic developmental disabilities in mammals are caused by errors in chromosome segregation originating mainly in the oocyte during meiosis I. Some conditions, like maternal ageing or in vitro maturation (IVM), increase the incidence of oocyte aneuploidy. Here oocytes from adult mares were used to investigate oocyte maturation in a monovulatory species. Experiments were conducted to compare: (1) the incidence of aneuploidy, (2) the morphology of the spindle, (3) the acetylation of lysine 16 on histone H4 (H4K16) and (4) the relative amount of histone acetyltransferase 1 (HAT1), K(lysine) acetyltransferase 8 (KAT8, also known as MYST1), histone deacetylase 1 (HDAC1) and NAD-dependent protein deacetylase sirtuin 1 (SIRT1) mRNA in metaphase II stage oocytes that were in vitro matured or collected from peri-ovulatory follicles. The frequency of aneuploidy and anomalies in spindle morphology was increased following IVM, along with a decrease in H4K16 acetylation that was in agreement with our previous observations. However, differences in the amount of the transcripts investigated were not detected. These results suggest that the degradation of transcripts encoding for histone deacetylases and acetyltransferases is not involved in the changes of H4K16 acetylation observed following IVM, while translational or post-Translational mechanisms might have a role. Our study also suggests that epigenetic instabilities introduced by IVM may affect the oocyte and embryo genetic stability. © CSIRO 2017.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Franciosi, Federica;  Reproductive and Developmental Biology Laboratory, Department of Health, University of Milan, via Celoria 10, Milan, Italy
Goudet, Ghylene;  INRA, UMR85 Physiologie de la Reproduction et des Comportements, Nouzilly, France, CNRS, UMR7247, Nouzilly, France, Université François Rabelais, 60 Rue du Plat d'Étain, Tours, France, IFCE, Nouzilly, France
Tessaro, Irene;  Reproductive and Developmental Biology Laboratory, Department of Health, University of Milan, via Celoria 10, Milan, Italy
Papillier, Pascal;  INRA, UMR85 Physiologie de la Reproduction et des Comportements, Nouzilly, France, CNRS, UMR7247, Nouzilly, France, Université François Rabelais, 60 Rue du Plat d'Étain, Tours, France, IFCE, Nouzilly, France
Dalbies-Tran, Rozenn;  INRA, UMR85 Physiologie de la Reproduction et des Comportements, Nouzilly, France, CNRS, UMR7247, Nouzilly, France, Université François Rabelais, 60 Rue du Plat d'Étain, Tours, France, IFCE, Nouzilly, France
Reigner, Fabrice;  INRA, UEPAO, Nouzilly, France
Deleuze, Stefan  ;  Université de Liège - ULiège > Dép. clinique des animaux de compagnie et des équidés (DCA) > Obstét. et path. de la reprod. des anim. de comp. et équidés
Douet, Cécile;  INRA, UMR85 Physiologie de la Reproduction et des Comportements, Nouzilly, France, CNRS, UMR7247, Nouzilly, France, Université François Rabelais, 60 Rue du Plat d'Étain, Tours, France, IFCE, Nouzilly, France
Miclea, Ileana;  University of Agricultural Sciences and Veterinary Medicine, Calea Mǎnǎtur 3-5, Cluj-Napoca, Romania
Lodde, Valentina;  Reproductive and Developmental Biology Laboratory, Department of Health, University of Milan, via Celoria 10, Milan, Italy
Luciano, Alberto M.;  Reproductive and Developmental Biology Laboratory, Department of Health, University of Milan, via Celoria 10, Milan, Italy
Language :
English
Title :
In vitro maturation affects chromosome segregation, spindle morphology and acetylation of lysine 16 on histone H4 in horse oocytes
Publication date :
2017
Journal title :
Reproduction, Fertility and Development
ISSN :
1031-3613
eISSN :
1448-5990
Publisher :
CSIRO
Volume :
29
Issue :
4
Pages :
721-730
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 27 February 2019

Statistics


Number of views
140 (4 by ULiège)
Number of downloads
257 (3 by ULiège)

Scopus citations®
 
14
Scopus citations®
without self-citations
11
OpenCitations
 
14

Bibliography


Similar publications



Contact ORBi