Article (Scientific journals)
Ovum Pick Up and In Vitro Maturation of Jennies Oocytes Toward the Setting Up of Efficient In Vitro Fertilization and In Vitro Embryos Culture Procedures in Donkey (Equus asinus)
Deleuze, Stefan; Douet, Cécile; Couty, I. et al.
2018In Journal of Equine Veterinary Science, 65, p. 111-117
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Keywords :
Donkey; IVF; IVM; Oocyte; Ovum pick up; Spermatozoa; Review
Abstract :
[en] Most wild and domestic donkey breeds are currently endangered or threatened. Their preservation includes the creation of a Genome Resource Bank. Embryo cryopreservation allows preservation of genetics from both male and female and is the fastest method to restore a breed. Embryo production in vivo is limited in equids. We recently established a technique of ovum pick up (OPU) in the donkey. Conditions of in vitro maturation (IVM), in vitro fertilization (IVF), and in vitro culture of zygotes have been evaluated. Equine abattoir–derived oocytes were used as controls. Donkey cumulus-oocyte complexes (COCs) collected by OPU were matured in vitro in TCM199 with fetal calf serum and epidermal growth factor for 24, 30, 34, or 38 hours. Forty-four percent were in metaphase 2 after 34 hours. In our conditions, IVM of donkey oocytes was slower than that of equine oocytes and the optimal duration for donkey oocytes IVM may be 34 hours. Oocytes we co-incubated with frozen-thawed donkey semen treated with procaine for 18 hours and cultured for 30 hours in a Dulbecco Modified Eagle Medium-F12-based medium. Only 15% of jennies oocytes contained 2 pronuclei after co-incubation, and none of them developed further after 48 hours after IVF. Treatment of donkey sperm with procaine may not be efficient for IVF. Some parthenogenetic activation occurred. In conclusion, we confirm that our conditions for OPU in jennies yielded high recovery rates that improved with operator experience. Maturation rates of 44% can be achieved using the IVM medium routinely used for equine oocytes in our lab. Further studies are in progress to establish efficient conditions for IVF and development of donkey zygotes. © 2018 Elsevier Inc.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Deleuze, Stefan  ;  Université de Liège - ULiège > Dép. clinique des animaux de compagnie et des équidés (DCA) > Obstét. et path. de la reprod. des anim. de comp. et équidés
Douet, Cécile;  INRA, UMR 85, Physiologie de la Reproduction et des Comportements, Nouzilly, France, CNRS, UMR 7247, Nouzilly, France, IFCE, Nouzilly, France, Université François Rabelais, Tours, France
Couty, I.;  INRA, UMR 85, Physiologie de la Reproduction et des Comportements, Nouzilly, France, CNRS, UMR 7247, Nouzilly, France, IFCE, Nouzilly, France, Université François Rabelais, Tours, France
Moros-Nicolás, C.;  INRA, UMR 85, Physiologie de la Reproduction et des Comportements, Nouzilly, France, CNRS, UMR 7247, Nouzilly, France, IFCE, Nouzilly, France, Université François Rabelais, Tours, France
Barrière, P.;  INRA, UE1297 Unité Expérimentale de Physiologie Animale de l'Orfrasière, Nouzilly, France
Blard, T.;  INRA, UE1297 Unité Expérimentale de Physiologie Animale de l'Orfrasière, Nouzilly, France
Reigner, Fabrice;  INRA, UE1297 Unité Expérimentale de Physiologie Animale de l'Orfrasière, Nouzilly, France
Magistrini, M.;  INRA, UMR 85, Physiologie de la Reproduction et des Comportements, Nouzilly, France, CNRS, UMR 7247, Nouzilly, France, IFCE, Nouzilly, France, Université François Rabelais, Tours, France
Goudet, Ghylene;  INRA, UMR 85, Physiologie de la Reproduction et des Comportements, Nouzilly, France, CNRS, UMR 7247, Nouzilly, France, IFCE, Nouzilly, France, Université François Rabelais, Tours, France
Language :
English
Title :
Ovum Pick Up and In Vitro Maturation of Jennies Oocytes Toward the Setting Up of Efficient In Vitro Fertilization and In Vitro Embryos Culture Procedures in Donkey (Equus asinus)
Publication date :
2018
Journal title :
Journal of Equine Veterinary Science
ISSN :
0737-0806
eISSN :
1542-7412
Publisher :
W.B. Saunders
Volume :
65
Pages :
111-117
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 27 February 2019

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