Article (Scientific journals)
Direct interactions between the secreted effector and the T2SS components GspL and GspM reveal a new effector-sensing step during type 2 secretion
Michel-Souzy, S.; Douzi, B.; Cadoret, F. et al.
2018In Journal of Biological Chemistry, 293 (50), p. 19441-19450
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Keywords :
Bacteria; Hybrid systems; Proteins; Direct interactions; Effector binding; Gram-negative bacteria; Homodimerization; Protein interaction networks; Pseudomonas aeruginosa; Secretion systems; Two-hybrid system; Physiology
Abstract :
[en] In many Gram-negative bacteria, the type 2 secretion system (T2SS) plays an important role in virulence because of its capacity to deliver a large amount of fully folded protein effectors to the extracellular milieu. Despite our knowledge of most T2SS components, the mechanisms underlying effector recruitment and secretion by the T2SS remain enigmatic. Using complementary biophysical and biochemical approaches, we identified here two direct interactions between the secreted effector CbpD and two components, XcpYL and XcpZM, of the T2SS assembly platform (AP) in the opportunistic pathogen Pseudomonas aeruginosa. Competition experiments indicated that CbpD binding to XcpYL is XcpZM-dependent, suggesting sequential recruitment of the effector by the periplasmic domains of these AP components. Using a bacterial two-hybrid system, we then tested the influence of the effector on the AP protein–protein interaction network. Our findings revealed that the presence of the effector modifies the AP interactome and, in particular, induces XcpZM homodimerization and increases the affinity between XcpYL and XcpZM. The observed direct relationship between effector binding and T2SS dynamics suggests an additional synchronizing step during the type 2 secretion process, where the activation of the AP of the T2SS nanomachine is triggered by effector binding. © 2018 by The American Society for Biochemistry and Molecular Biology, Inc.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Michel-Souzy, S.;  CNRS, Aix Marseille Université, Institut de Microbiologie de la Méditerranée (IMM), Laboratoire d’Ingénierie des Systèmes Macromoléculaires (LISM)/UMR7255, Marseille, 13009, France
Douzi, B.;  CNRS, Aix Marseille Université, Institut de Microbiologie de la Méditerranée (IMM), Laboratoire d’Ingénierie des Systèmes Macromoléculaires (LISM)/UMR7255, Marseille, 13009, France, CNRS, Aix Marseille Université, IMM, Laboratoire de Chimie Bactérienne (LCB)/UMR7283, Marseille, 13009, France
Cadoret, F.;  CNRS, Aix Marseille Université, Institut de Microbiologie de la Méditerranée (IMM), Laboratoire d’Ingénierie des Systèmes Macromoléculaires (LISM)/UMR7255, Marseille, 13009, France
Raynaud, C.;  CNRS, Aix Marseille Université, Institut de Microbiologie de la Méditerranée (IMM), Laboratoire d’Ingénierie des Systèmes Macromoléculaires (LISM)/UMR7255, Marseille, 13009, France, CNRS, Aix Marseille Université, IMM, Laboratoire de Chimie Bactérienne (LCB)/UMR7283, Marseille, 13009, France
Quinton, Loïc  ;  Université de Liège - ULiège > Département de chimie (sciences) > Chimie biologique
Ball, G.;  CNRS, Aix Marseille Université, Institut de Microbiologie de la Méditerranée (IMM), Laboratoire d’Ingénierie des Systèmes Macromoléculaires (LISM)/UMR7255, Marseille, 13009, France, CNRS, Aix Marseille Université, IMM, Laboratoire de Chimie Bactérienne (LCB)/UMR7283, Marseille, 13009, France
Voulhoux, R.;  CNRS, Aix Marseille Université, Institut de Microbiologie de la Méditerranée (IMM), Laboratoire d’Ingénierie des Systèmes Macromoléculaires (LISM)/UMR7255, Marseille, 13009, France, CNRS, Aix Marseille Université, IMM, Laboratoire de Chimie Bactérienne (LCB)/UMR7283, Marseille, 13009, France
Language :
English
Title :
Direct interactions between the secreted effector and the T2SS components GspL and GspM reveal a new effector-sensing step during type 2 secretion
Publication date :
2018
Journal title :
Journal of Biological Chemistry
ISSN :
0021-9258
eISSN :
1083-351X
Publisher :
American Society for Biochemistry and Molecular Biology Inc.
Volume :
293
Issue :
50
Pages :
19441-19450
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 26 February 2019

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