Regulation of the Peptidoglycan Polymerase Activity of PBP1b by Antagonist Actions of the Core Divisome Proteins FtsBLQ and FtsN

Boes, Adrien; olatunji, Samir; Breukink, Eefjan; Terrak, Mohammed

doi:10.1128/mBio.01912-18

Article (Scientific journals)

Regulation of the Peptidoglycan Polymerase Activity of PBP1b by Antagonist Actions of the Core Divisome Proteins FtsBLQ and FtsN

Boes, Adrien; olatunji, Samir; Breukink, Eefjan et al.

2019 • In MBio, 10

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/232984

DOI
10.1128/mBio.01912-18

PubMed
30622193

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Keywords :

peptidoglycan; interaction; protein; divisome; Lipid II; FtsN; FtsBLQ; PBP1b; PBP3; FtsW; LpoB; membrane

Abstract :

[en] Bacterial cell division is governed by a multiprotein complex called divisome, which facilitates a precise cell wall synthesis at midcell and daughter cell separation. Protein-protein interactions and activity studies using different combinations of the septum synthesis core of the divisome revealed that the glycosyltransferase activity of PBP1b is repressed by FtsBLQ and that the presence of FtsN or LpoB suppresses this inhibition. Moreover, FtsBLQ also inhibits the PBP3 activity on a thioester substrate. These results provide enzymatic evidence of the regulation of the peptidoglycan synthase PBP1b and PBP3 within the divisome. The results confirm that PBP1b plays an important role in E. coli cell division and shed light on the specific role of FtsN, which functions to relieve the repression on PBP1b by FtsBLQ and to initiate septal peptidoglycan synthesis.

Research center :

CIP - Centre d'Ingénierie des Protéines - ULiège

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Boes, Adrien ; Université de Liège - ULiège > Département des sciences de la vie > Centre d'ingénierie des protéines

olatunji, Samir

Breukink, Eefjan

Terrak, Mohammed ; Université de Liège - ULiège > Département des sciences de la vie > Centre d'ingénierie des protéines

Language :

English

Title :

Regulation of the Peptidoglycan Polymerase Activity of PBP1b by Antagonist Actions of the Core Divisome Proteins FtsBLQ and FtsN

Alternative titles :

[fr] Regulation de l'activité de synthèse du peptidoglycane du PBP1b par l'action antagoniste de FtBLQ et FtsN

Publication date :

08 January 2019

Journal title :

MBio

ISSN :

2161-2129

eISSN :

2150-7511

Publisher :

American Society for Microbiology, Washington, United States - Washington

Volume :

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://mbio.asm.org/content/10/1/e01912-18

Name of the research project :

Divisome

Funders :

F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]

Available on ORBi :

since 17 February 2019

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