Genomic characterization and pathogenic study of two porcine reproductive and respiratory syndrome viruses with different virulence in Fujian, China

Xiukun, Sui; Ting, Xin; Xiaoyu, Guo; Hong, Jia; Ming, Li; Xintao, Gao; Jing, Wu; Yitong, Jiang; Willems, Luc; Hongfei, Zhu; Shaohua, Hou

doi:10.4142/jvs.2018.19.3.339

Article (Scientific journals)

Genomic characterization and pathogenic study of two porcine reproductive and respiratory syndrome viruses with different virulence in Fujian, China

Xiukun, Sui; Ting, Xin; Xiaoyu, Guo et al.

2018 • In Journal of Veterinary Science, 19 (3), p. 339-349

Peer reviewed

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https://hdl.handle.net/2268/232848

DOI
10.4142/jvs.2018.19.3.339

PubMed
29284210

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Keywords :

genomics; phylogeny; porcine respiratory and reproductive syndrome virus; virulence

Abstract :

[en] Two strains of porcine reproductive and respiratory syndrome virus (PRRSV) were isolated in 2006 and 2016 and designated as FZ06A and FZ16A, respectively. Inoculation experiments showed that FZ06A caused 100% morbidity and 60% mortality, while FZ16A caused 100% morbidity without death. By using genomic sequence and phylogenetic analyses, close relationships between a Chinese highly pathogenic PRRSV strain and the FZ06A and FZ16A strains were observed. Based on the achieved results, multiple genomic variations in Nsp2, a unique N-glycosylation site (N33→K33), and a K151 amino acid (AA) substitution for virulence in the GP5 of FZ16A were detected; except the 30 AA deletion in the Nsp2-coding region. Inoculation experiments were conducted and weaker virulence of FZ16A than FZ06A was observed. Based on our results, a 30 AA deletion in the Nsp2-coding region is an unreliable genomic indicator of a high virulence PRRSV strain. The Nsp2 and GP5 differences, in addition to the virulence difference between these two highly pathogenic PRRSV strains, have the potential to be used to establish a basis for further study of PRRSV virulence determinants and to provide data useful in the development of vaccines against this economically devastating disease.

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Xiukun, Sui

Ting, Xin

Xiaoyu, Guo

Hong, Jia

Ming, Li

Xintao, Gao

Jing, Wu

Yitong, Jiang

Willems, Luc ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies

Hongfei, Zhu

Shaohua, Hou

Language :

English

Title :

Genomic characterization and pathogenic study of two porcine reproductive and respiratory syndrome viruses with different virulence in Fujian, China

Publication date :

2018

Journal title :

Journal of Veterinary Science

ISSN :

1229-845X

eISSN :

1976-555X

Publisher :

Korean Society of Veterinary Science, South Korea

Volume :

Issue :

Pages :

339-349

Peer reviewed :

Peer reviewed

Additional URL :

https://doi.org/10.4142/jvs.2018.19.3.339

Available on ORBi :

since 12 February 2019

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Bibliography

Allende, R., Kutish, G.F., Laegreid, W., Lu, Z., Lewis, T.L., Rock, D.L., Friesen, J., Osorio, F.A., Mutations in the genome of porcine reproductive and respiratory syndrome virus responsible for the attenuation phenotype (2000) Arch Virol, 145, pp. 1149-1161
An, T.Q., Tian, Z.J., Leng, C.L., Peng, J.M., Tong, G.Z., Highly pathogenic porcine reproductive and respiratory syndrome virus, Asia (2011) Emerg Infect Dis, 17, pp. 1782-1784
Ansari, I.H., Kwon, B., Osorio, F.A., Pattnaik, A.K., Influence of N-linked glycosylation of porcine reproductive and respiratory syndrome virus GP5 on virus infectivity, antigenicity, and ability to induce neutralizing antibodies (2006) J Virol, 80, pp. 3994-4004
Benfield, D.A., Nelson, E., Collins, J.E., Harris, L., Goyal, S.M., Robison, D., Christianson, W.T., Chladek, D., Characterization of swine infertility and respiratory syndrome (SIRS) virus (isolate ATCC VR-2332) (1992) J Vet Diagn Invest, 4, pp. 127-133
Chen, Z., Zhou, X., Lunney, J.K., Lawson, S., Sun, Z., Brown, E., Christopher-Hennings, J., Fang, Y., Immunodominant epitopes in nsp2 of porcine reproductive and respiratory syndrome virus are dispensable for replication, but play an important role in modulation of the host immune response (2010) J Gen Virol, 91, pp. 1047-1057
Collins, J.E., Benfield, D.A., Christianson, W.T., Harris, L., Hennings, J.C., Shaw, D.P., Goyal, S.M., Chladek, D., Isolation of swine infertility and respiratory syndrome virus (isolate ATCC VR-2332) in North America and experimental reproduction of the disease in gnotobiotic pigs (1992) J Vet Diagn Invest, 4, pp. 117-126
Dea, S., Gagnon, C.A., Mardassi, H., Pirzadeh, B., Rogan, D., Current knowledge on the structural proteins of porcine reproductive and respiratory syndrome (PRRS) virus: comparison of the North American and European isolates (2000) Arch Virol, 145, pp. 659-688
Du, Y., Lu, Y., Qi, J., Wu, J., Wang, G., Wang, J., Complete genome sequence of a moderately pathogenic porcine reproductive and respiratory syndrome virus variant strain (2012) J Virol, 86, pp. 13883-13884
Fan, B., Wang, H., Bai, J., Zhang, L., Jiang, P., A novel isolate with deletion in GP3 gene of porcine reproductive and respiratory syndrome virus from mid-eastern China (2014) Biomed Res Int, 2014
Fang, Y., Fang, L., Wang, Y., Lei, Y., Luo, R., Wang, D., Chen, H., Xiao, S., Porcine reproductive and respiratory syndrome virus nonstructural protein 2 contributes to NF-κB activation (2012) Virol J, 9, p. 83
Fang, Y., Kim, D.Y., Ropp, S., Steen, P., Christopher-Hennings, J., Nelson, E.A., Rowland, R.R., Heterogeneity in Nsp2 of European-like porcine reproductive and respiratory syndrome viruses isolated in the United States (2004) Virus Res, 100, pp. 229-235
Firth, A.E., Zevenhoven-Dobbe, J.C., Wills, N.M., Go, Y.Y., Balasuriya, U.B., Atkins, J.F., Snijder, E.J., Posthuma, C.C., Discovery of a small arterivirus gene that overlaps the GP5 coding sequence and is important for virus production (2011) J Gen Virol, 92, pp. 1097-1106
Forsberg, R., Storgaard, T., Nielsen, H.S., Oleksiewicz, M.B., Cordioli, P., Sala, G., Hein, J., Bøtner, A., The genetic diversity of European type PRRSV is similar to that of the North American type but is geographically skewed within Europe (2002) Virology, 299, pp. 38-47
Halbur, P.G., Paul, P.S., Frey, M.L., Landgraf, J., Eernisse, K., Meng, X.J., Lum, M.A., Rathje, J.A., Comparison of the pathogenicity of two US porcine reproductive and respiratory syndrome virus isolates with that of the Lelystad virus (1995) Vet Pathol, 32, pp. 648-660
Huang, C., Zhang, Q., Feng, W.H., Regulation and evasion of antiviral immune responses by porcine reproductive and respiratory syndrome virus (2015) Virus Res, 202, pp. 101-111
Jiang, P., Chen, P.Y., Dong, Y.Y., Cai, J.L., Cai, B.X., Jiang, Z.H., Isolation and genome characterization of porcine reproductive and respiratory syndrome virus in P.R China (2000) J Vet Diagn Invest, 12, pp. 156-158
Johnson, C.R., Griggs, T.F., Gnanandarajah, J., Murtaugh, M.P., Novel structural protein in porcine reproductive and respiratory syndrome virus encoded by an alternative ORF5 present in all arteriviruses (2011) J Gen Virol, 92, pp. 1107-1116
Karniychuk, U.U., Geldhof, M., Vanhee, M., Van Doorsselaere, J., Saveleva, T.A., Nauwynck, H.J., Pathogenesis and antigenic characterization of a new East European subtype 3 porcine reproductive and respiratory syndrome virus isolate (2010) BMC Vet Res, 6, p. 30
Ladinig, A., Detmer, S.E., Clarke, K., Ashley, C., Rowland, R.R., Lunney, J.K., Harding, J.C., Pathogenicity of three type 2 porcine reproductive and respiratory syndrome virus strains in experimentally inoculated pregnant gilts (2015) Virus Res, 203, pp. 24-35
Li, B., Xiao, S., Wang, Y., Xu, S., Jiang, Y., Chen, H., Fang, L., Immunogenicity of the highly pathogenic porcine reproductive and respiratory syndrome virus GP5 protein encoded by a synthetic ORF5 gene (2009) Vaccine, 27, pp. 1957-1963
Li, Y., Xue, C., Wang, L., Chen, X., Chen, F., Cao, Y., Genomic analysis of two Chinese strains of porcine reproductive and respiratory syndrome viruses with different virulence (2010) Virus Genes, 40, pp. 374-381
Ma, H., Kien, F., Manière, M., Zhang, Y., Lagarde, N., Tse, K.S., Poon, L.L., Nal, B., Human annexin A6 interacts with influenza a virus protein M2 and negatively modulates infection (2012) J Virol, 86, pp. 1789-1801
Meulenberg, J.J., PRRSV, the virus (2000) Vet Res, 31, pp. 11-21
Murtaugh, M.P., Faaberg, K.S., Laber, J., Elam, M., Kapur, V., Genetic variation in the PRRS virus (1998) Adv Exp Med Biol, 440, pp. 787-794
Nelsen, C.J., Murtaugh, M.P., Faaberg, K.S., Porcine reproductive and respiratory syndrome virus comparison: divergent evolution on two continents (1999) J Virol, 73, pp. 270-280
Ni, J., Yang, S., Bounlom, D., Yu, X., Zhou, Z., Song, J., Khamphouth, V., Tian, K., Emergence and pathogenicity of highly pathogenic Porcine reproductive and respiratory syndrome virus in Vientiane, Lao People's Democratic Republic (2012) J Vet Diagn Invest, 24, pp. 349-354
Ostrowski, M., Galeota, J.A., Jar, A.M., Platt, K.B., Osorio, F.A., Lopez, O.J., Identification of neutralizing and nonneutralizing epitopes in the porcine reproductive and respiratory syndrome virus GP5 ectodomain (2002) J Virol, 76, pp. 4241-4250
Paton, D.J., Brown, I.H., Edwards, S., Wensvoort, G., 'Blue ear' disease of pigs (1991) Vet Rec, 128, p. 617
Shi, M., Lam, T.T., Hon, C.C., Murtaugh, M.P., Davies, P.R., Hui, R.K., Li, J., Leung, F.C., Phylogeny-based evolutionary, demographical, and geographical dissection of North American type 2 porcine reproductive and respiratory syndrome viruses (2010) J Virol, 84, pp. 8700-8711
Snijder, E.J., Meulenberg, J.J., The molecular biology of arteriviruses (1998) J Gen Virol, 79, pp. 961-979
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., Kumar, S., MEGA5: Molecular Evolutionary Genetics Analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods (2011) Mol Biol Evol, 28, pp. 2731-2739
Tian, K., Yu, X., Zhao, T., Feng, Y., Cao, Z., Wang, C., Hu, Y., Gao, G.F., Emergence of fatal PRRSV variants: unparalleled outbreaks of atypical PRRS in China and molecular dissection of the unique hallmark (2007) PLoS One, 2
Tong, G.Z., Zhou, Y.J., Hao, X.F., Tian, Z.J., An, T.Q., Qiu, H.J., Highly pathogenic porcine reproductive and respiratory syndrome, China (2007) Emerg Infect Dis, 13, pp. 1434-1436
Vu, H.L., Kwon, B., Yoon, K.J., Laegreid, W.W., Pattnaik, A.K., Osorio, F.A., Immune evasion of porcine reproductive and respiratory syndrome virus through glycan shielding involves both glycoprotein 5 as well as glycoprotein 3 (2011) J Virol, 85, pp. 5555-5564
Wang, F.X., Song, N., Chen, L.Z., Cheng, S.P., Wu, H., Wen, Y.J., Non-structural protein 2 of the porcine reproductive and respiratory syndrome (PRRS) virus: a crucial protein in viral pathogenesis, immunity and diagnosis (2013) Res Vet Sci, 95, pp. 1-7
Wang, X., Marthaler, D., Rovira, A., Rossow, S., Murtaugh, M.P., Emergence of a virulent porcine reproductive and respiratory syndrome virus in vaccinated herds in the United States (2015) Virus Res, 210, pp. 34-41
Zhang, G., Lu, W., Chen, Y., Zhu, L., Wei, Z., Li, Z., Sun, B., Ma, J., Complete genome sequence of two variant porcine reproductive and respiratory syndrome viruses isolated from vaccinated piglets (2012) J Virol, 86, pp. 11396-11397
Zhou, L., Chen, S., Zhang, J., Zeng, J., Guo, X., Ge, X., Zhang, D., Yang, H., Molecular variation analysis of porcine reproductive and respiratory syndrome virus in China (2009) Virus Res, 145, pp. 97-105
Zhou, L., Zhang, J., Zeng, J., Yin, S., Li, Y., Zheng, L., Guo, X., Yang, H., The 30-amino-acid deletion in the Nsp2 of highly pathogenic porcine reproductive and respiratory syndrome virus emerging in China is not related to its virulence (2009) J Virol, 83, pp. 5156-5167
Zhou, Z., Li, X., Liu, Q., Hu, D., Yue, X., Ni, J., Yu, X., Tian, K., Complete genome sequence of two novel Chinese virulent porcine reproductive and respiratory syndrome virus variants (2012) J Virol, 86, pp. 6373-6374