Article (Scientific journals)
Genomic characterization and pathogenic study of two porcine reproductive and respiratory syndrome viruses with different virulence in Fujian, China
Xiukun, Sui; Ting, Xin; Xiaoyu, Guo et al.
2018In Journal of Veterinary Science, 19 (3), p. 339-349
Peer reviewed
 

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Keywords :
genomics; phylogeny; porcine respiratory and reproductive syndrome virus; virulence
Abstract :
[en] Two strains of porcine reproductive and respiratory syndrome virus (PRRSV) were isolated in 2006 and 2016 and designated as FZ06A and FZ16A, respectively. Inoculation experiments showed that FZ06A caused 100% morbidity and 60% mortality, while FZ16A caused 100% morbidity without death. By using genomic sequence and phylogenetic analyses, close relationships between a Chinese highly pathogenic PRRSV strain and the FZ06A and FZ16A strains were observed. Based on the achieved results, multiple genomic variations in Nsp2, a unique N-glycosylation site (N33→K33), and a K151 amino acid (AA) substitution for virulence in the GP5 of FZ16A were detected; except the 30 AA deletion in the Nsp2-coding region. Inoculation experiments were conducted and weaker virulence of FZ16A than FZ06A was observed. Based on our results, a 30 AA deletion in the Nsp2-coding region is an unreliable genomic indicator of a high virulence PRRSV strain. The Nsp2 and GP5 differences, in addition to the virulence difference between these two highly pathogenic PRRSV strains, have the potential to be used to establish a basis for further study of PRRSV virulence determinants and to provide data useful in the development of vaccines against this economically devastating disease.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Xiukun, Sui
Ting, Xin
Xiaoyu, Guo
Hong, Jia
Ming, Li
Xintao, Gao
Jing, Wu
Yitong, Jiang
Willems, Luc  ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies
Hongfei, Zhu
Shaohua, Hou
Language :
English
Title :
Genomic characterization and pathogenic study of two porcine reproductive and respiratory syndrome viruses with different virulence in Fujian, China
Publication date :
2018
Journal title :
Journal of Veterinary Science
ISSN :
1229-845X
eISSN :
1976-555X
Publisher :
Korean Society of Veterinary Science, South Korea
Volume :
19
Issue :
3
Pages :
339-349
Peer reviewed :
Peer reviewed
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since 12 February 2019

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