Ultra-high-performance liquid chromatography-mass spectrometry method for neutrophil gelatinase-associated lipocalin as a predictive biomarker in acute kidney injury

Ion, Valentin; Nys, Gwenaël; COBRAIVILLE, Gaël; Cavalier, Etienne; Crommen, Jacques; Servais, Anne-Catherine; Muntean, D.-L.; Fillet, Marianne

doi:10.1016/j.talanta.2018.11.050

Article (Scientific journals)

Ultra-high-performance liquid chromatography-mass spectrometry method for neutrophil gelatinase-associated lipocalin as a predictive biomarker in acute kidney injury

Ion, Valentin; Nys, Gwenaël; COBRAIVILLE, Gaël et al.

2019 • In Talanta, 195, p. 668-675

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/232657

DOI
10.1016/j.talanta.2018.11.050

PubMed
30625599

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Keywords :

AKI; NGAL; Targeted proteomics; UHPLC-MS/MS; Body fluids; High performance liquid chromatography; Mass spectrometry; Molecular biology; Phase separation; Human plasma samples; Internal standards; Neutrophil gelatinase-associated lipocalin; Solid-phase extraction; U-HPLC-MS; Ultra high performance liquid chromatography-mass spectrometries; Proteins

Abstract :

[en] Neutrophil gelatinase associated lipocalin (NGAL) is a protein that was found to be overexpressed in acute kidney injury (AKI). The rise in NGAL concentration, both in urine or plasma, appears earlier than for other classical renal function markers such as serum creatinine, thus making it a suitable marker for early pathology detection. The aim of this study was to develop a method involving tryptic digestion, solid phase extraction and LC-MS/MS analysis to analyze NGAL in plasma medium using an isotope labeled surrogate protein, containing NGAL signature tags, as internal standard (QPrEST). The method was validated for the analysis of NGAL in an analytical range from 50 to 1250 ng/mL using two different proteotypic peptides. The method was further used to quantify the NGAL in human plasma samples for whom elevated NGAL values were expected. NGAL values were between 190.8 and 242.6 ng/mL for control group and between 228.1 and 3526.2 ng/mL for patient group. This study proved that the selection of the right internal standard is of utmost importance in targeted proteomics studies as the digestion steps might cause high variability. This study also confirmed that, although NGAL is highly resistant to proteases such as trypsin, the method could be fully validated according to FDA guidelines and subsequently used to assess NGAL levels in patient plasma with high analytical confidence. © 2018

Research Center/Unit :

CIRM - Centre Interdisciplinaire de Recherche sur le Médicament - ULiège

Disciplines :

Pharmacy, pharmacology & toxicology

Author, co-author :

Ion, Valentin ; Université de Liège - ULiège > Doct. sc. bioméd. & pharma. (paysage)

Nys, Gwenaël ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments

COBRAIVILLE, Gaël ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments

Cavalier, Etienne ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale

Crommen, Jacques ; Université de Liège - ULiège > Département de pharmacie > Département de pharmacie

Servais, Anne-Catherine ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments

Muntean, D.-L.; Analytical Chemistry and Drug Analysis Department, Faculty of Pharmacy, University of Medicine, Pharmacy, Sciences and Technology from Tîrgu Mureș, Tîrgu Mureș, 540139, Romania

Fillet, Marianne ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments

Language :

English

Title :

Ultra-high-performance liquid chromatography-mass spectrometry method for neutrophil gelatinase-associated lipocalin as a predictive biomarker in acute kidney injury

Publication date :

2019

Journal title :

Talanta

ISSN :

0039-9140

eISSN :

1873-3573

Publisher :

Elsevier B.V.

Volume :

195

Pages :

668-675

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 07 February 2019

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Bibliography

Grebe, S.K.G., Singh, R.J., Clinical peptide and protein quantification by mass spectrometry (MS). TrAC Trends Anal. Chem., 2016, 10.1016/j.trac.2016.01.026.
Himmelsbach, M., 10 years of MS instrumental developments–impact on LC-MS/MS in clinical chemistry. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 883–884 (2012), 3–17, 10.1016/j.jchromb.2011.11.038.
Rauh, M., LC-MS/MS for protein and peptide quantification in clinical chemistry. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 883–884 (2012), 59–67, 10.1016/j.jchromb.2011.09.030.
Thomas, A.A., Demirjian, S., Lane, B.R., Simmons, M.N., Goldfarb, D.A., Subramanian, V.S., Campbell, S.C., Acute kidney injury: novel biomarkers and potential utility for patient care in urology. Urology 77 (2011), 5–11, 10.1016/j.urology.2010.05.004.
Bellomo, R., Kellum, J.A., Ronco, C., Acute kidney injury. Lancet 380 (2012), 756–766, 10.1016/S0140-6736(11)61454-2.
Edelstein, C.L., Biomarkers of kidney disease. Edelstein, Charles L., (eds.) Biomarkers Kidney Dis., first ed., 2011, Academic Press, Elsevier, London, San Diego, 179–211.
Gueguen, Y., Rouas, C., Leblond, F. a., Kidney injury biomarkers. Néphrologie Thérapeutique 8 (2012), 146–155, 10.1016/j.nephro.2012.02.004.
Oezkur, M., Magyar, A., Thomas, P., Stork, T., Schneider, R., Bening, C., Störk, S., Heuschmann, P.U., Leyh, R.G., Wagner, M., TIMP-2*IGFBP7 (Nephrocheck®) measurements at intensive care unit admission after cardiac surgery are predictive for acute kidney injury within 48 h. Kidney Blood Press. Res., 2017, 456–467, 10.1159/000479298.
Roszyk, L., Sapin, V., Neutrophil gelatinase-associated lipocalin (NGAL): caractéristiques immuno-analytiques. Immuno-Anal. Biol. Spécialisée 27 (2012), 365–368, 10.1016/j.immbio.2012.08.002.
Zhao, C., Ozaeta, P., Fishpaugh, J., Rupprecht, K., Workman, R., Grenier, F., Ramsay, C., Structural characterization of glycoprotein NGAL, an early predictive biomarker for acute kidney injury. Carbohydr. Res. 345 (2010), 2252–2261, 10.1016/j.carres.2010.07.024.
Alvelos, M., Lourenço, P., Dias, C., Amorim, M., Rema, J., Leite, A.B., Guimarães, J.T., Almeida, P., Bettencourt, P., Prognostic value of neutrophil gelatinase-associated lipocalin in acute heart failure. Int. J. Cardiol. 165 (2013), 51–55, 10.1016/j.ijcard.2011.07.080.
Hamouche, E.M., Revue sur la neutrophil gelatinase associated lipocalin ou NGAL, Immuno-Analyse Biol. Spécialisée 27 (2012), 149–152, 10.1016/j.immbio.2012.03.002.
Parikh, C.R., Devarajan, P., Zappitelli, M., Sint, K., Thiessen-Philbrook, H., Li, S., Kim, R.W., Koyner, J.L., Coca, S.G., Edelstein, C.L., Shlipak, M.G., Garg, A.X., Krawczeski, C.D., Thiessen-Philbrook, H., Shlipak, M.G., Koyner, J.L., Wang, Z., Edelstein, C.L., Devarajan, P., Patel, U.D., Zappitelli, M., Krawczeski, C.D., Passik, C.S., Swaminathan, M., Garg, A.X., Postoperative biomarkers predict acute kidney injury and poor outcomes after adult cardiac surgery. J. Am. Soc. Nephrol. 22 (2011), 1737–1747, 10.1681/ASN.2010111163.
Mishra, J., Dent, C., Tarabishi, R., Mitsnefes, M.M., Ma, Q., Kelly, C., Ruff, S.M., Zahedi, K., Shao, M., Bean, J., Mori, K., Barasch, J., Devarajan, P., Neutrophil gelatinase-associated lipocalin (NGAL) as a biomarker for acute renal injury after cardiac surgery. Lancet 365 (2005), 1231–1238, 10.1016/S0140-6736(05)74811-X.
Camou, F., Oger, S., Paroissin, C., Guilhon, E., Guisset, O., Mourissoux, G., Pouyes, H., Lalanne, T., Gabinski, C., Plasma Neutrophil Gelatinase-Associated Lipocalin (NGAL) predicts acute kidney injury in septic shock at ICU admission. Ann. Fr. Anesth. Reanim. 32 (2013), 157–164, 10.1016/j.annfar.2012.11.012.
De Geus, H.R.H., Bakker, J., Lesaffre, E.M.E.H., Noble, J.L.M.L., Neutrophil gelatinase-associated lipocalin at ICU admission predicts for acute kidney injury in adult patients. Am. J. Respir. Crit. Care Med. 183 (2011), 907–914, 10.1164/rccm.200908-1214OC.
Zhang, X., Gibson, B., Mori, R., Snow-Lisy, D., Yamaguchi, Y., Campbell, S.C., Simmons, M.N., Daly, T.M., Analytical and biological validation of a multiplex immunoassay for acute kidney injury biomarkers. Clin. Chim. Acta 415 (2013), 88–93, 10.1016/j.cca.2012.09.022.
Grenier, F.C., Ali, S., Syed, H., Workman, R., Martens, F., Liao, M., Wang, Y., Wong, P.-Y., Evaluation of the ARCHITECT urine NGAL assay: assay performance, specimen handling requirements and biological variability. Clin. Biochem. 43 (2010), 615–620, 10.1016/j.clinbiochem.2009.12.008.
Kannan, P., Tiong, H.Y., Kim, D.-H., Highly sensitive electrochemical determination of neutrophil gelatinase-associated lipocalin for acute kidney injury. Biosens. Bioelectron. 31 (2012), 32–36, 10.1016/j.bios.2011.09.036.
Vashist, S.K., Saraswat, M., Holthšfer, H., Development of a rapid sandwich enzyme linked immunoassay procedure for the highly sensitive detection of human lipocalin-2/NGAL. Procedia Chem. 6 (2012), 141–148, 10.1016/j.proche.2012.10.140.
Domanski, D., Percy, A.J., Yang, J., Chambers, A.G., Hill, J.S., Freue, G.V.C., Borchers, C.H., MRM-based multiplexed quantitation of 67 putative cardiovascular disease biomarkers in human plasma. Proteomics 12 (2012), 1222–1243, 10.1002/pmic.201100568.
MacLean, B., Tomazela, D.M., Shulman, N., Chambers, M., Finney, G.L., Frewen, B., Kern, R., Tabb, D.L., Liebler, D.C., MacCoss, M.J., Skyline: an open source document editor for creating and analyzing targeted proteomics experiments. Bioinformatics 26 (2010), 966–968, 10.1093/bioinformatics/btq054.
Mohammed, Y., Domański, D., Jackson, A.M., Smith, D.S., Deelder, A.M., Palmblad, M., Borchers, C.H., PeptidePicker: a scientific workflow with web interface for selecting appropriate peptides for targeted proteomics experiments. J. Proteomics, 2014, 151–161, 10.1016/j.jprot.2014.04.018.
Broad Institute of MIT and Harvard, Spectrum Mill MS Proteomics Workbench, 2014.
van den Broek, I., Sparidans, R.W., Schellens, J.H.M., Beijnen, J.H., Quantitative bioanalysis of peptides by liquid chromatography coupled to (tandem) mass spectrometry. J. Chromatogr. B 872 (2008), 1–22, 10.1016/j.jchromb.2008.07.021.
Brice, R.W., Zhang, X., Colón, L.A., Fused-core, sub-2 μm packings, and monolithic HPLC columns: a comparative evaluation. J. Sep. Sci. 32 (2009), 2723–2731, 10.1002/jssc.200900091.
Schuster, S.A., Boyes, B.E., Wagner, B.M., Kirkland, J.J., Fast high performance liquid chromatography separations for proteomic applications using Fused-Core® silica particles. J. Chromatogr. A 1228 (2012), 232–241, 10.1016/J.CHROMA.2011.07.082.
Marchi, I., Viette, V., Badoud, F., Fathi, M., Saugy, M., Rudaz, S., Veuthey, J.L., Characterization and classification of matrix effects in biological samples analyses. J. Chromatogr. A 1217 (2010), 4071–4078, 10.1016/j.chroma.2009.08.061.
Chambers, E., Wagrowski-Diehl, D.M., Lu, Z., Mazzeo, J.R., Systematic and comprehensive strategy for reducing matrix effects in LC/MS/MS analyses. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 852 (2007), 22–34, 10.1016/j.jchromb.2006.12.030.
Houbart, V., Cobraiville, G., Lecomte, F., Debrus, B., Hubert, P., Fillet, M., Development of a nano-liquid chromatography on chip tandem mass spectrometry method for high-sensitivity hepcidin quantitation. J. Chromatogr. A 1218 (2011), 9046–9054, 10.1016/j.chroma.2011.10.030.
Proc, J.L., Kuzyk, M.A., Hardie, D.B., Yang, J., Smith, D.S., Jackson, A.M., Parker, C.E., Borchers, C.H., Proc, et al. SI3. A quantitative study of the effects of chaotropic agents, surfactants and solvents on the digestion efficiency of human plasma proteins by trypsin. J. Proteome Res., 2009, 5422–5437, 10.1021/pr100656u.
Walmsley, S.J., Rudnick, P.A., Liang, Y., Dong, Q., Stein, S.E., Nesvizhskii, A.I., Comprehensive analysis of protein digestion using six trypsins reveals the origin of trypsin as a significant source of variability in proteomics. J. Proteome Res. 12 (2013), 5666–5680, 10.1021/pr400611h.
Kjeldsen, L., Johnsen, A.H., Sengeløv, H., Borregaard, N., Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase. J. Biol. Chem. 268 (1993), 10425–10432.
Lin, Y., Zhou, J., Bi, D., Chen, P., Wang, X., Liang, S., Sodium-deoxycholate-assisted tryptic digestion and identification of proteolytically resistant proteins. Anal. Biochem. 377 (2008), 259–266, 10.1016/j.ab.2008.03.009.
FDA - Guidance for Industry Bioanalytical Method Validation, USA, n.d.

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