Reference : Establishment of reference intervals for serum concentrations of androstanediol glucu...
Scientific journals : Article
Human health sciences : Laboratory medicine & medical technology
http://hdl.handle.net/2268/232155
Establishment of reference intervals for serum concentrations of androstanediol glucuronide by a newly developed LC-MS/MS method.
English
Fabregat-Cabello, Neus [> >]
PEETERS, Stéphanie mailto [Centre Hospitalier Universitaire de Liège - CHU > Unilab > Laboratoire techniques séparatives et stress oxydant >]
Yilmaz, Tugba mailto [Université de Liège - ULiège > > > Master bioch. & biol. mol. & cel., à fin.]
Cavalier, Etienne mailto [Université de Liège - ULiège > Département de pharmacie > Chimie médicale >]
Le Goff, Caroline mailto [Université de Liège - ULiège > Département de pharmacie > Chimie médicale >]
2019
Steroids
143
62-66
Yes (verified by ORBi)
International
0039128X
United States
[en] Androstanediol glucuronide ; Liquid chromatography ; Mass spectrometry ; Reference intervals ; Steroids
[en] BACKGROUND: Androstanediol glucuronide is linked to a range of disorders of peripheral androgen formation and action, such as in hirsutism and acne. Nowadays its accurate quantification is still challenging and there are just a few LC-MS/MS methods available. Besides, their reference intervals for normal European populations by LC-MS/MS, including prepubertal and pubertal children, have not been reported yet. METHODS: Validation of the proposed new methodology was performed at 3 levels in triplicate during 3 different days. Calibration curve concentration ranged from 0.1 to 25microg/L. For method comparison between ELISA and the newly developed LC-MS/MS method, 43 patient samples were tested. A reference interval study was performed with 264 healthy Belgian individuals (108 male and 156 female). RESULTS: Validation of the proposed LC-MS/MS method was satisfactorily achieved, with mean imprecision values lower than 7.4%, mean recoveries within 99-108% and a limit of quantification of 0.059microg/L. Compared to LC-MS/MS, ELISA showed a positive bias in serum samples, providing results 43% higher for the same sample. As a consequence, new reference intervals based on age and gender have been calculated. CONCLUSION: An easy, fast and straightforward LC-MS/MS method for the determination of androstanediol glucuronide has been developed and fully validated. Besides, reference interval for normal European populations, including prepubertal and pubertal children has been established for the first time.
http://hdl.handle.net/2268/232155
10.1016/j.steroids.2018.12.008
Copyright (c) 2019 Elsevier Inc. All rights reserved.

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