Article (Scientific journals)
Resistance of Isolated Pulmonary Mitochondria During in Vitro Anoxia/Reoxygenation
Willet, K.; Detry, Olivier; Sluse, Francis
2000In Biochimica et Biophysica Acta, 1460 (2-3), p. 346-52
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Keywords :
organ transplantation; lung; ischemia
Abstract :
[en] The aim of the study was to investigate the effect of in vitro anoxia/reoxygenation on the oxidative phosphorylation of isolated lung mitochondria. Mitochondria were isolated after harvesting from fresh pig lungs flushed with Euro-Collins solution. Mitochondrial respiratory parameters were determined in isolated mitochondria before anoxia (control), after 5-45 min anoxia followed by 5 min reoxygenation, and after 25 or 40 min of in vitro incubation in order to follow the in vitro aging of mitochondria during respiratory assays. Respiratory parameters measured after anoxia/reoxygenation did not show any oxidative phosphorylation dysfunction, indicating a high resistance of pulmonary mitochondria to in vitro anoxia/reoxygenation (up to 45 min anoxia). These results indicate that mitochondria are not directly responsible of their oxidative phosphorylation damage observed after in vivo ischemia (K. Willet et al., Transplantation 69 (2000) 582) but are a target of others cellular injuries leading to mitochondrial dysfunction in vivo.
Disciplines :
Biochemistry, biophysics & molecular biology
Surgery
Author, co-author :
Willet, K.
Detry, Olivier  ;  Centre Hospitalier Universitaire de Liège - CHU > Chirurgie abdominale- endocrinienne et de transplantation
Sluse, Francis ;  Université de Liège - ULiège > Département des sciences de la vie > Bioénergétique et physiologie cellulaire
Language :
English
Title :
Resistance of Isolated Pulmonary Mitochondria During in Vitro Anoxia/Reoxygenation
Publication date :
20 November 2000
Journal title :
Biochimica et Biophysica Acta
ISSN :
0006-3002
eISSN :
1878-2434
Publisher :
Elsevier, Amsterdam, Netherlands
Volume :
1460
Issue :
2-3
Pages :
346-52
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 22 September 2009

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