Article (Scientific journals)
CRISPR/Cas9-Mediated Immunity to Geminiviruses: Differential Interference and Evasion.
Ali, Zahir; Ali, Shakila; Tashkandi, Manal et al.
2016In Scientific Reports, 6, p. 26912
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Keywords :
Base Sequence; CRISPR-Cas Systems; DNA End-Joining Repair; Disease Resistance; Geminiviridae/genetics/immunology; Genes, Viral; INDEL Mutation; Inverted Repeat Sequences; Open Reading Frames; Plant Diseases/immunology/virology; Tobacco/genetics/immunology/virology; Viral Proteins/genetics
Abstract :
[en] The CRISPR/Cas9 system has recently been used to confer molecular immunity against several eukaryotic viruses, including plant DNA geminiviruses. Here, we provide a detailed analysis of the efficiencies of targeting different coding and non-coding sequences in the genomes of multiple geminiviruses. Moreover, we analyze the ability of geminiviruses to evade the CRISPR/Cas9 machinery. Our results demonstrate that the CRISPR/Cas9 machinery can efficiently target coding and non-coding sequences and interfere with various geminiviruses. Furthermore, targeting the coding sequences of different geminiviruses resulted in the generation of viral variants capable of replication and systemic movement. By contrast, targeting the noncoding intergenic region sequences of geminiviruses resulted in interference, but with inefficient recovery of mutated viral variants, which thus limited the generation of variants capable of replication and movement. Taken together, our results indicate that targeting noncoding, intergenic sequences provides viral interference activity and significantly limits the generation of viral variants capable of replication and systemic infection, which is essential for developing durable resistance strategies for long-term virus control.
Disciplines :
Agriculture & agronomy
Genetics & genetic processes
Biotechnology
Biochemistry, biophysics & molecular biology
Author, co-author :
Ali, Zahir
Ali, Shakila
Tashkandi, Manal
Syed-Shan-e-Ali, Zaïdi ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Ingénierie des productions végétales et valorisation
Mahfouz, Magdy M.
Language :
English
Title :
CRISPR/Cas9-Mediated Immunity to Geminiviruses: Differential Interference and Evasion.
Publication date :
2016
Journal title :
Scientific Reports
eISSN :
2045-2322
Publisher :
Nature Publishing Group, London, United Kingdom
Volume :
6
Pages :
26912
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 19 January 2019

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