Effect of nicotine on rat gingival fibroblasts in vitro

[en] Nicotine from 3 to 5 mM affects growth and survival rate of rat gingival fibroblasts in vitro. Ultrastructural analysis revealed dilated mitochondria and vacuolization in treated cells, suggestive of necrosis, but increased apoptosis was also revealed by cytometry. On the basis of this in vitro study, it appear that tobacco, through its component nicotine, may directly affect various functions of rat gingival fibroblasts

Disciplines :

Anatomy (cytology, histology, embryology...) & physiology

Author, co-author :

Lahmouzi, Jamila ; Centre Hospitalier Universitaire de Liège - CHU > Médecine dentaire

Simain-Sato, Franklin ; Centre Hospitalier Universitaire de Liège - CHU > Médecine dentaire

Defresne, Marie-Paule ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie - Cytologie

De Pauw-Gillet, Marie-Claire ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie - Cytologie - Département des sciences biomédicales et précliniques

Heinen, Ernst ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine

Grisar, Thierry ; Centre Hospitalier Universitaire de Liège - CHU > Neurologie Sart Tilman

Legros, Jean-Jacques ; Université de Liège - ULiège > Département des sciences cliniques > Département des sciences cliniques

Legrand, Roman ; Université de Liège - ULiège > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine)

Language :

English

Title :

Effect of nicotine on rat gingival fibroblasts in vitro

Publication date :

2000

Journal title :

Connective Tissue Research

ISSN :

0300-8207

eISSN :

1607-8438

Publisher :

Taylor & Francis, Philadelphia, United States - Pennsylvania

Volume :

Issue :

Pages :

69-80

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 30 June 2011

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Bibliography

Haber, J. and Wattles, J. (1994). Evidence for cigarette smoking as a major risk factor for periodontitis. J. Periodontol., 64, 16-23.
Frandsen, A. and Pindborg, J. (1949). Tobacco and gingivitis. III. Difference in action of cigarette and pipe smoking. J. Dent. Res., 28, 464-465.
Österberg, T. and Mellström, D. (1986). A major risk factor for loss of teeth in the 70-year-old cohorts. Community Dent. Oral. Epidemiol., 14, 367-370.
Ragnarsson, E. and Eliasson, S.T. (1992). Tobacco smoking, a factor in tooth loss in Reykjavik, Iceland. Scand. J. Dent. Res., 100, 322-326.
Bolin, A. and Lavsted, S. (1986). Proximal alveolar bone loss in a longitudinal radiographic investigation. IV. Smoking and other factors influence the progress of individuals with at least 20 remaining teeth. Acta Odontol. Scand., 44, 263-269.
Linden, G.J. and Mullaly, B.H. (1994). Cigarette smoking and periodontal destruction in young adults. J. Periodontol., 65, 718-723.
Feldmen, R.S. and Alman, J.E. (1987). Periodontal disease indexes and tobacco smoking in healthy aging men. Gerodontics, 3, 43-46.
Hanes, P.J. et al. (1991). Binding, uptake, and release of nicotine by human gingival fibroblasts. J. Periodontol., 62, 147-152.
Freshney, R. (1983). Culture of animal cells. In: A Manual of Basic Technique. New York: Alan R. Liss, Inc.
Labarca, C. et al. (1980). A simple, rapid and sensitive DNA assay procedure. Anal. Biochem., 102, 344.
Page, R.C. et al. (1985). Effect of inflammation on the distribution of collagen types I, III, IV, V and type I trimmer and fibronectin in human gingiva. J. Dent. Res., 64(9), 1111-1116.
Juva, K. et al. (1966). Modified procedure for the assay of 3H- or 14C-labeled hydroxyproline. Anal. Biochem., 15, 77.
Lambert, C.A. et al. (1992). Pretranslational regulation of extracellular matrix macromoleculcs and collagenase expression in fibroblasts by mechanical forces. Lab. Invest., 66(4), 44.
Peacock, M.E. et al. (1993). The effect of nicotine on reproduction and attachment of human gingival fibroblasts in vitro. J. Periodontol., 64, 658-665.
Mangan, F.D. et al. (1993). Apoptosis in human monocytes: Possible role in chronic inflammatory diseases. J. Periodontol., 64, 461-466.
Greimers, R. et al. (1996). Improve four-colour flow cytometry method using Fluo-3 and triple immunofluorescence for analysis of intracellular calcium ion (Ca2+). Flux among mouse lymph node B- and T-lymphocyte subsets. Cytometry, 23, 205-217.
Axell, T. and Mornstadt, H. (1976). The relation of the clinical picture to the histopathology of snuff dippers lesion in a Swedish population. J. Oral. Pathol., 5, 229-236.
Feldmen, R.S. and Alman, J.E. (1987). Periodontal diseases indices and tobacco smoking in healthy aging men. Gerodontics, 1, 43-46.
Hedin, C.A. and Ronquist, G. (1981). Cyclic nucleotide content of gingival tissue of smokers and non-smokers. J. Periodont. Res., 16, 337-343.
Grossi, S.G. et al. (1994). Assessment of risk for periodontal disease. I. Risk indicator for attachment loss. J. Periodontol., 65, 260-267.
Grossi, S.G. et al. (1995). Assessment of risk for periodontal disease. II. Risk indicator for alveolar bone loss. J. Periodontol., 66, 23-29.
Horning, G.M. et al. (1992). Risk indicators for periodontitis in a military treatment population. J. Periodontol., 63, 297-302.
Romanos, G. et al. (1991). Immunohistochemical distribution of the collagen types IV, V, VI and glycoprotein laminin in the healthy rat, marmoset and human gingiva. Matrix Vol., 11, 125-132.
Chavrier, C. et al. (1989). Immunohistochemical study of types I, II and IV collagen in diseased human gingiva of patients with rapidly progressive periodontitis: A light and election microscopic study. Cell. Molecul. Biol., 35, 457-467.
Chavrier, C. et al. (1984). Connective tissue organization of healthy human gingiva. Ultrastructural localization of collagen types I III IV. J. Periodont. Res., 19, 221-229.
Burgeson, R.E. et al. (1988). New collagens, new concepts. Ann. Rev. Cell Biol., 4, 551-577.
Tipton, D. et al. (1995). Effect of nicotine on proliferation and extracellular matrix production of human gingival fibroblasts in vitro. J. Periodontol., 66, 1056-1064.
Bordin, S. et al. (1984). Heterogeneity of normal human diploid fibroblasts: Isolation and characterization of one phenotype. Science, 223, 171-173.
Arends, M.J. et al. (1991). Apoptosis: Mechanism and roles in pathology. Int. Rev. Exp. Path., 32, 223-254.
Cohen, J.J. et al. (1991). Programmed cell death in the immune system. Adv. Immunol., 50, 55-85.
Waring, P. et al. (1991). Apoptosis or programmed cell death. Medicinal Res. Rev., 11, 219-236.
Savil, J.S. et al. (1989). Macrophage phagocytosis of aging neutrophils in inflammation. Programmed cell death in the neutrophils leads to its recognition by macrophages. J. Clin. Invest., 83, 865-875.
Hoffman, D. and Adams, J. (1981). Carcinogenic tobacco-specific N-nitrosamines in snuff and in the saliva of snuff dippers. Canc. Res., 41, 4305-4308.