Article (Scientific journals)
Highly informative multiclass profiling of lipids by ultra-high performance liquid chromatography – Low resolution (quadrupole) mass spectrometry by using electrospray ionization and atmospheric pressure chemical ionization interfaces
Beccaria, M.; Inferrera, V.; Rigano, F. et al.
2017In Journal of Chromatography. A, 1509, p. 69-82
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Keywords :
Atmospheric pressure chemical ionization; Human plasma; Lipid fragmentation; Lipidomics; Lipids; Low resolution mass spectrometry; Atmospheric chemistry; Atmospheric ionization; Atmospheric movements; Atmospheric pressure; Chemical modification; Chromatography; High performance liquid chromatography; Ionization; Ionization of liquids; Liquid chromatography; Mass spectrometers; Mass spectrometry; Negative ions; Plasma (human); Spectrometry; Chromatographic conditions; Electrospray ionization interface; Fragmentation patterns; Human plasmas; Low resolution mass spectrometries; Ultra-high performance liquid chromatographies; Electrospray ionization; Article; Chromatography, High Pressure Liquid; Humans; Indicators and Reagents; Spectrometry, Mass, Electrospray Ionization
Abstract :
[en] A simple, fast, and versatile method, using an ultra-high performance liquid chromatography system coupled with a low resolution (single quadrupole) mass spectrometer was optimized to perform multiclass lipid profiling of human plasma. Particular attention was made to develop a method suitable for both electrospray ionization and atmospheric pressure chemical ionization interfaces (sequentially in positive- and negative-ion mode), without any modification of the chromatographic conditions (mobile phase, flow-rate, gradient, etc.). Emphasis was given to the extrapolation of the structural information based on the fragmentation pattern obtained using atmospheric pressure chemical ionization interface, under each different ionization condition, highlighting the complementary information obtained using the electrospray ionization interface, of support for related molecule ions identification. Furthermore, mass spectra of phosphatidylserine and phosphatidylinositol obtained using the atmospheric pressure chemical ionization interface are reported and discussed for the first time. © 2017 Elsevier B.V.
Disciplines :
Biochemistry, biophysics & molecular biology
Chemistry
Author, co-author :
Beccaria, M.;  Chromaleont s.r.l., Messina, Italy
Inferrera, V.;  Department of “Scienze Chimiche, Biologiche Farmaceutiche ed Ambientali, University of Messina, Messina, Italy
Rigano, F.;  Chromaleont s.r.l., Messina, Italy
Gorynski, K.;  Department of Pharmacodynamics and Molecular Pharmacology, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, Poland
Purcaro, Giorgia  ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie des agro-biosystèmes
Pawliszyn, J.;  Department of Chemistry, 200 University Avenue, West, Waterloo, Ontario, Canada
Dugo, P.;  Chromaleont s.r.l., Messina, Italy, Department of “Scienze Chimiche, Biologiche Farmaceutiche ed Ambientali, University of Messina, Messina, Italy, University Campus Bio-Medico of Rome, Roma, Italy
Mondello, L.;  Chromaleont s.r.l., Messina, Italy, Department of “Scienze Chimiche, Biologiche Farmaceutiche ed Ambientali, University of Messina, Messina, Italy, University Campus Bio-Medico of Rome, Roma, Italy
Language :
English
Title :
Highly informative multiclass profiling of lipids by ultra-high performance liquid chromatography – Low resolution (quadrupole) mass spectrometry by using electrospray ionization and atmospheric pressure chemical ionization interfaces
Publication date :
2017
Journal title :
Journal of Chromatography. A
ISSN :
0021-9673
eISSN :
1873-3778
Publisher :
Elsevier
Volume :
1509
Pages :
69-82
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 17 January 2019

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