Free fatty acid profiling of marine sentinels by nanoLC-EI-MS for the assessment of environmental pollution effects

Bioindicator; Biomonitoring; Environmental pollution; Free fatty acids (FFAs); M. galloprovincialis; NanoLC-EI-MS; Biomarkers; Ionization of liquids; Liquid chromatography; Marine pollution; Mass spectrometry; Molluscs; Nanotechnology; Phase separation; Pollution; Environmental pollutions; Free fatty acid; Nano-LC; Fatty acids; Article; Italy; Mytilus galloprovincialis; Mytilus; Animals; Chromatography, Liquid; Environmental Monitoring; Fatty Acids, Nonesterified; Water Pollutants, Chemical

Abstract :

[en] The present work aims to elucidate the free fatty acid (FFA) profile of the mussel Mytilus galloprovincialis caged in an anthropogenically impacted area and in a reference site through an innovative and validated analytical approach for the assessment of biological alterations induced by marine pollution. The FFA pattern is involved in the regulation of different cellular pathways and differs with respect to metabolic stimuli. To this purpose, the lipid fraction of mussels coming from both sampling areas was extracted and the FFA fractions were isolated and purified by a solid phase extraction; then, nano-scale liquid chromatography coupled to electron ionization mass spectrometry (nanoLC-EI-MS) was employed for the characterization of the two samples. A total of 19 and 17 FFAs were reliably identified in the mussels coming from the reference and polluted site, respectively. Significant qualitative and quantitative differences found in saturated, monounsaturated and polyunsaturated species may be exploited as typical pollution biomarkers (e.g. alteration of the fatty acid biosynthetic system and lipotoxicity) and explain adverse and compromising effects (e.g. oxidative stress and inflammatory processes) related to environmental pollution. © 2016 Elsevier B.V.

Disciplines :

Chemistry
Animal production & animal husbandry

Author, co-author :

Albergamo, A.; Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, University of Messina, Polo Annunziata - viale Annunziata, Messina, 98168, Italy

Rigano, F.; Chromaleont s.r.l., c/o University of Messina, Polo Annunziata - viale Annunziata, Messina, 98168, Italy

Purcaro, Giorgia ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie des agro-biosystèmes

Mauceri, A.; Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, University of Messina, Polo Annunziata - viale Annunziata, Messina, 98168, Italy

Fasulo, S.; Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, University of Messina, Polo Annunziata - viale Annunziata, Messina, 98168, Italy

Mondello, L.; Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, University of Messina, Polo Annunziata - viale Annunziata, Messina, 98168, Italy, Chromaleont s.r.l., c/o University of Messina, Polo Annunziata - viale Annunziata, Messina, 98168, Italy, Centro Integrato di Ricerca, University Campus Bio-Medico of Rome, Via Álvaro del Portillo, 21, Rome, 00128, Italy

Language :

English

Title :

Free fatty acid profiling of marine sentinels by nanoLC-EI-MS for the assessment of environmental pollution effects

Publication date :

2016

Journal title :

Science of the Total Environment

ISSN :

0048-9697

eISSN :

1879-1026

Publisher :

Elsevier B.V.

Volume :

571

Pages :

955-962

Peer reviewed :

Peer Reviewed verified by ORBi

Name of the research project :

National Interest Research Project (PRIN 2010–2011, Prot. 2010ARBLT7_001/008)

Available on ORBi :

since 17 January 2019

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Bibliography

Ausili, A., Gabellini, M., Cammarata, G., Fattorini, D., Benedetti, M., Pisanelli, B., Gorbi, S., Regoli, F., Ecotoxicological and human health risk in a petrochemical district of southern Italy. Mar. Environ. Res. 66 (2008), 215–217.
Azzaro, F., Concentrazioni Di Nutrienti Nella Baia Di Augusta (Sicilia). Atti I Workshop “Risorsa Mare: Un Progetto per Il Mezzogiorno. Salvaguardia e Valorizzazione Delle Acque Costiere Del Salento”, 8 Luglio 1991. Lecce, Italy, 1993, 93–108.
Bebianno, M.J., Lopes, B., Guerra, L., Hoarau, P., Ferreira, A.M., Glutathione S-tranferases and cytochrome P450 activities in Mytilus galloprovincialis from the South coast of Portugal: effect of abiotic factors. Environ. Int. 33 (2007), 550–558.
Berg, A.H., Terry, P., Scherer, P.E., ACRP30/adiponectin: an adipokine regulating glucose and lipid metabolism. Trends Endocrinol. Metab. 13 (2002), 84–89.
Bligh, E.G., Dyer, W.J., A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37 (1959), 911–917.
Boutet, I., Tanguy, A., Moraga, D., Response of the Pacific oyster Crassostrea gigas to hydrocarbon contamination under experimental conditions. Gene 329 (2004), 147–157.
Cappello, T., Maisano, M., Agata, A.D., Natalotto, A., Mauceri, A., Fasulo, S., Effects of environmental pollution in caged mussels (Mytilus galloprovincialis). Mar. Environ. Res. 91 (2013), 52–60.
Cappello, T., Mauceri, A., Corsaro, C., Maisano, M., Parrino, V., Lo Paro, G., Messina, G., Fasulo, S., Impact of environmental pollution on caged mussels Mytilus galloprovincialis using NMR-based metabolomics. Mar. Pollut. Bull. 77 (2013), 132–139.
Cappello, T., Brandão, F., Guilherme, S., Santos, M.A., Maisano, M., Mauceri, A., Canário, J., Pacheco, M., Pereira, P., Insights into the mechanisms underlying mercury-induced oxidative stress in gills of wild fish (Liza aurata) combining 1H NMR metabolomics and conventional biochemical assays. Sci. Total Environ. 548–549 (2016), 13–24.
Cedergreen, N., Quantifying synergy: a systematic review of mixture toxicity studies within environmental toxicology. PLoS One, 9(5), 2014, e96580.
Cook, H.W., McMaster, C.R., Fatty Acid Desaturation and Chain Elongation in Eukaryotes. Vance, J.E., Vance, D.E., (eds.) Biochemistry of Lipids, Lipoproteins and Membranes, 2008, Elsevier, New York, 181–204.
Costa, R., Beccaria, M., Grasso, E., Albergamo, A., Oteri, M., Dugo, P., Fasulo, S., Mondello, L., Sample preparation techniques coupled to advanced chromatographic methods for marine organisms investigation. Anal. Chim. Acta 875 (2015), 41–53.
Costa, R., Albergamo, A., Piparo, M., Zaccone, G., Capillo, G., Manganaro, A., Dugo, P., Mondello, L., Multidimensional gas chromatographic techniques applied to the analysis of lipids from wild-caught and farmed marine species. Eur. J. Lipid Sci. Technol., 2016, 10.1002/ejlt.201600043.
D.M. 10.01.2000. Decreto Ministeriale 10.01 del 23.02.2000. Perimetrazione del sito di interesse nazionale di Gela e Priolo. G.U.N. 44.
D.M. 468/2001. Decreto Ministeriale del 18.09.2001 n.468. Programma nazionale di bonifica e ripristino ambientale. G.U.N. 13.
D.M. 56/2009. Decreto ministeriale del 14.04.2009 n.56. Regolamento recante criteri tecnici per il monitoraggio dei corpi idrici e l'identificazione delle condizioni di riferimento per la modifica delle norme tecniche del decreto legislativo 3 aprile 2006, n. 152, recante norme in materia ambientale, predisposto ai sensi dell'articolo 75, comma 3, del decreto legislativo medesimo. G:U.N. 124.
Di Leonardo, R., Mazzola, A., Tramati, C.D., Vaccaro, A., Vizzini, S., Highly contaminated areas as sources of pollution for adjoining ecosystems: the case of Augusta Bay (Central Mediterranean). Mar. Pollut. Bull. 89 (2014), 417–426.
Donaldson, R.P., Membrane lipid metabolism in germinating Castor bean endosperm. Plant Physiol. 57 (1976), 510–515.
European Commission, Directive 2455/2001/EC of the European Parliament and of the Council of 20 November 2001 establishing the list of priority substances in the field of water policy and amending Directive 2000/60/EC. Off. J. Eur. Communities 15 (2001), 1–5.
European Commission, Directive 2004/35/EC of the European Parliament and of the Council of 21 April 2004 on environmental liability with regard to the prevention and remedying of environmental damage. Off. J. Eur. Union L 143 (2004), 56–75.
European Commission, Directive 2008/56/EC of the European Parliament and of the Council of 17 June 2008 establishing a framework for community action in the field of marine environmental policy (Marine Strategy Framework Directive). Off. J. Eur. Union L 164 (2008), 19–40.
Fokina, N.N., Ruokolainen, T.R., Nemova, N.N., Bakhmet, I.N., Changes of blue mussels Mytilus edulis L. lipid composition under cadmium and copper toxic effect. Biol. Trace Elem. Res. 154 (2013), 217–225.
Giacobbe, S., Leopardi, M., Rinelli, P., Aspetto biologico e sedimentario dei fondi mobili della Rada di Augusta (Sicilia sudorientale). Nova Thalassia 10 (1989), 133–149.
Gorbi, S., Lamberti, C.V., Notti, A., Benedetti, M., Fattorini, D., Moltedo, G., Regoli, F., An ecotoxicological protocol with caged mussels, Mytilus galloprovincialis, for monitoring the impact of an offshore platform in the Adriatic sea. Mar. Environ. Res. 65:1 (2008), 34–49.
Jones, G.J., Nichols, P.D., Johns, R.B., Smith, J.D., The effect of mercury and cadmium on the fatty acid and sterol composition of the marine diatom Asterionella glacialis. Phytochemistry 26 (1987), 1343–1348.
Juàrez, M., Polvillo, O., Contò, M., Ficco, A., Ballico, S., Failla, S., Comparison of four extraction/methylation analytical methods to measure fatty acid composition by gas chromatography in meat. J. Chromatogr. A 1190 (2008), 327–332.
Kudo, N., Waku, K., Cadmium suppresses A9 desaturase activity in rat hepatocytes. Toxicology 114 (1996), 101–111.
Labarta, U., Fernández-Reiriz, M.J., Garrido, J.L., Babarro, J.M.F., Bayona, J.M., Albaigés, J., Response of mussel recruits to pollution from the “Prestige” oil spill along the Galicia coast. A biochemical approach. Mar. Ecol. Prog. Ser. 302 (2005), 135–145.
Lavarías, S., Pollero, R.J., Heras, H., Activation of lipid catabolism by the water-soluble fraction of petroleum in the crustacean Macrobrachium borellii. Aquat. Toxicol. 77 (2006), 190–196.
Maisano, M., Cappello, T., Natalotto, A., Vitale, V., Parrino, V., Giannetto, A., Oliva, S., Mancini, G., Cappello, S., Mauceri, A., Fasulo, S., Effects of petrochemical contamination on caged marine mussels using a multi-biomarker approach: histological changes, neurotoxicity and hypoxic stress. Mar. Environ. Res., 2016 (in press).
Marchi, B., Burlando, B., Moore, M.N., Viarengo, A., Mercury- and copper-induced lysosomal membrane destabilisation depends on [Ca2 +]i dependent phospholipase A2 activation. Aquat. Toxicol. 66 (2004), 197–204.
Miyazaki, M., Kim, H.-J., Man, W.C., Ntambi, J.M., Oleoyl-CoA is the major de novo product of stearoyl-CoA desaturase 1 Gene isoform and substrate for the biosynthesis of the Harderian gland 1-alkyl-2,3-diacylglycerol. J. Biol. Chem. 276 (2001), 39455–39461.
OECD TG 305, Bioaccumulation in fish: aqueousand dietary exposure. 2012 Paris.
Papackova, Z., Cahova, M., Fatty acid signaling: the new function of intracellular lipases. Int. J. Mol. Sci. 16 (2015), 3831–3855.
Parrish, C.C., Lipids in marine ecosystems. ISRN Oceanogr. 2013 (2013), 1–16.
Perrat, E., Couzinet-Mossion, A., Fossi Tankoua, O., Amiard-Triquet, C., Wielgosz-Collin, G., Variation of content of lipid classes, sterols and fatty acids in gonads and digestive glands of Scrobicularia plana in relation to environment pollution levels. Ecotoxicol. Environ. Saf. 90 (2013), 112–120.
Quehenberger, O., Armando, A.M., Dennis, E.A., High sensitivity quantitative lipidomics analysis of fatty acids in biological samples by gas chromatography–mass spectrometry. Biochim. Biophys. Acta 1811 (2011), 648–656.
Rigano, F., Albergamo, A., Sciarrone, D., Beccaria, M., Purcaro, G., Mondello, L., Nano liquid chromatography directly coupled to electron ionization mass spectrometry for free fatty acid elucidation in mussel. Anal. Chem. 88 (2016), 4021–4028.
Ruiz-Rodriguez, A., Reglero, G., Ibañez, E., Recent trends in the advanced analysis of bioactive fatty acids. J. Pharm. Biomed. Anal. 51:2 (2010), 305–326.
Russo, M., Rigano, F., Arigò, A., Calabrò, M.L., Farnetti, S., Dugo, P., Mondello, L., Rapid isolation, reliable characterization and water solubility improvement of polymethoxyflavones from cold-pressed mandarin essential oil. J. Sep. Sci. 39 (2016), 2018–2027.
Surendranath, P., Ramana Rao, K.V., Kelthane residues in tissues of the tropical penaeid prawn, Metapenaeus monoceros (Fabricius), under sublethal chronic exposure - a monitoring study. J. Appl. Toxicol. 11 (1991), 219–222.
Tithof, P.K., Elgayyar, M., Cho, Y., Guan, W.E.I., Fisher, A.B., Peters-golden, M., Polycyclic aromatic hydrocarbons present in cigarette smoke cause endothelial cell apoptosis by a phospholipase A2-dependent mechanism. FASEB J. 1 (2002), 1463–1464.
Treschow, A.P., Hodges, L.D., Wright, P.F.A., Wynne, P.M., Kalafatis, N., Macrides, T.A., Novel anti-inflammatory ω-3 PUFAs from the New Zealand green-lipped mussel, Perna canaliculus. Comp. Biochem. Physiol. B 147 (2007), 645–656.
Trufelli, H., Famiglini, G., Termopoli, V., Cappiello, A., Profiling of non-esterified fatty acids in human plasma using liquid chromatography-electron ionization mass spectrometry. Anal. Bioanal. Chem. 400 (2011), 2933–2941.
Viarengo, A., Lowe, D., Bolognesi, C., Fabbri, E., Koehler, A., The use of biomarkers in biomonitoring: a 2-tier approach assessing the level of pollutant-induced stress syndrome in sentinel organisms. Comp. Biochem. Physiol. C 146 (2007), 281–300.
Wall, R., Ross, R.P., Fitzgerald, G.F., Stanton, C., Fatty acids from fish: the anti-inflammatory potential of long-chain omega-3 fatty acids. Nutr. Rev. 68 (2010), 280–289.
Wang, Y.L., Liang, Y.Z., Chen, B.M., High-performance liquid chromatography with atmospheric pressure chemical ionization and electrospray ionization mass spectrometry for analysis of Angelica sinensis. Phytochem. Anal. 18:4 (2007), 265–274.
Wei, Y., Wang, D., Topczewski, F., Pagliassotti, M.J., Saturated fatty acids induce endoplasmic reticulum stress and apoptosis independently of ceramide in liver cells. Am. J. Physiol. Endocrinol. Metab. 291 (2006), E275–E281.
Zhou, Q., Zhang, J., Fu, J., Shi, J., Jiang, G., Biomonitoring: an appealing tool for assessment of metal pollution in the aquatic ecosystem. Anal. Chim. Acta 606 (2008), 135–150.