Free fatty acid profiling of marine sentinels by nanoLC-EI-MS for the assessment of environmental pollution effects

Bioindicator; Biomonitoring; Environmental pollution; Free fatty acids (FFAs); M. galloprovincialis; NanoLC-EI-MS; Biomarkers; Ionization of liquids; Liquid chromatography; Marine pollution; Mass spectrometry; Molluscs; Nanotechnology; Phase separation; Pollution; Environmental pollutions; Free fatty acid; Nano-LC; Fatty acids; Article; Italy; Mytilus galloprovincialis; Mytilus; Animals; Chromatography, Liquid; Environmental Monitoring; Fatty Acids, Nonesterified; Water Pollutants, Chemical

Abstract :

[en] The present work aims to elucidate the free fatty acid (FFA) profile of the mussel Mytilus galloprovincialis caged in an anthropogenically impacted area and in a reference site through an innovative and validated analytical approach for the assessment of biological alterations induced by marine pollution. The FFA pattern is involved in the regulation of different cellular pathways and differs with respect to metabolic stimuli. To this purpose, the lipid fraction of mussels coming from both sampling areas was extracted and the FFA fractions were isolated and purified by a solid phase extraction; then, nano-scale liquid chromatography coupled to electron ionization mass spectrometry (nanoLC-EI-MS) was employed for the characterization of the two samples. A total of 19 and 17 FFAs were reliably identified in the mussels coming from the reference and polluted site, respectively. Significant qualitative and quantitative differences found in saturated, monounsaturated and polyunsaturated species may be exploited as typical pollution biomarkers (e.g. alteration of the fatty acid biosynthetic system and lipotoxicity) and explain adverse and compromising effects (e.g. oxidative stress and inflammatory processes) related to environmental pollution. © 2016 Elsevier B.V.

Disciplines :

Chemistry
Animal production & animal husbandry

Author, co-author :

Albergamo, A.; Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, University of Messina, Polo Annunziata - viale Annunziata, Messina, 98168, Italy

Rigano, F.; Chromaleont s.r.l., c/o University of Messina, Polo Annunziata - viale Annunziata, Messina, 98168, Italy

Purcaro, Giorgia ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie des agro-biosystèmes

Mauceri, A.; Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, University of Messina, Polo Annunziata - viale Annunziata, Messina, 98168, Italy

Fasulo, S.; Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, University of Messina, Polo Annunziata - viale Annunziata, Messina, 98168, Italy

Mondello, L.; Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, University of Messina, Polo Annunziata - viale Annunziata, Messina, 98168, Italy, Chromaleont s.r.l., c/o University of Messina, Polo Annunziata - viale Annunziata, Messina, 98168, Italy, Centro Integrato di Ricerca, University Campus Bio-Medico of Rome, Via Álvaro del Portillo, 21, Rome, 00128, Italy

Language :

English

Title :

Free fatty acid profiling of marine sentinels by nanoLC-EI-MS for the assessment of environmental pollution effects

Publication date :

2016

Journal title :

Science of the Total Environment

ISSN :

0048-9697

eISSN :

1879-1026

Publisher :

Elsevier B.V.

Volume :

571

Pages :

955-962

Peer reviewed :

Peer Reviewed verified by ORBi

Name of the research project :

National Interest Research Project (PRIN 2010–2011, Prot. 2010ARBLT7_001/008)

Available on ORBi :

since 17 January 2019

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