Evaluation of differentiated bone cells proliferation by blue shark skin collagen via biochemical for bone tissue engineering

Elango, J.; Lee, J.; Wang, S.; Henrotin, Yves; De Val, J. E. M. S.; Regenstein, J. M.; Lim, S. Y.; Bao, B.; Wu, W.

doi:10.3390/md16100350

Article (Scientific journals)

Evaluation of differentiated bone cells proliferation by blue shark skin collagen via biochemical for bone tissue engineering

Elango, J.; Lee, J.; Wang, S. et al.

2018 • In Marine Drugs, 16 (10), p. 350

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DOI
10.3390/md16100350

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30257422

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Keywords :

Blue shark collagen; Differentiated mesenchymal stem cell; Osteoblast; Osteogenic activity; Proliferation; Runx2

Abstract :

[en] Collagen from a marine resource is believed to have more potential activity in bone tissue engineering and their bioactivity depends on biochemical and structural properties. Considering the above concept, pepsin soluble collagen (PSC) and acid soluble collagen (ASC) from blue shark (Prionace glauca) skin were extracted and its biochemical and osteogenic properties were investigated. The hydroxyproline content was higher in PSC than ASC and the purified collagens contained three distinct bands α1, α2 and β dimer. The purity of collagen was confirmed by the RP-HPLC profile and the thermogravimetric data showed a two-step thermal degradation pattern. ASC had a sharp decline in viscosity at 20–30◦C. Scanning electron microscope (SEM) images revealed the fibrillar network structure of collagens. Proliferation rates of the differentiated mouse bone marrow-mesenchymal stem (dMBMS) and differentiated osteoblastic (dMC3T3E1) cells were increased in collagen treated groups rather than the controls and the effect was dose-dependent, which was further supported by higher osteogenic protein and mRNA expression in collagen treated bone cells. Among two collagens, PSC had significantly increased dMBMS cell proliferation and this was materialized through increasing RUNX2 and collagen-I expression in bone cells. Accordingly, the collagens from blue shark skin with excellent biochemical and osteogenic properties could be a suitable biomaterial for therapeutic application. © 2018 MDPI AG. All rights reserved.

Disciplines :

Rheumatology

Author, co-author :

Elango, J.; Department of Marine Bio-Pharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, China

Lee, J.

Wang, S.; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Huaihai Institute of Technology, Lianyungang, 222005, China

Henrotin, Yves ; Université de Liège - ULiège > Département des sciences de la motricité > Unité de recherche sur l'os et le cartilage (U.R.O.C.)

De Val, J. E. M. S.; Department of Biomaterials Engineering, Universidad Católica San Antonio de Murcia, Murcia, 30107, Spain

Regenstein, J. M.; Department of Food Science, Cornell University, Ithaca, NY 14853-7201, United States

Lim, S. Y.; Division of Marine Bioscience, Korea Maritime and Ocean University, Busan, 606791, South Korea

Bao, B.; Department of Marine Bio-Pharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, China

Wu, W.; Department of Marine Bio-Pharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, China, Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Huaihai Institute of Technology, Lianyungang, 222005, China, Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation, Ministry of Agriculture, Shanghai, 201306, China

Language :

English

Title :

Evaluation of differentiated bone cells proliferation by blue shark skin collagen via biochemical for bone tissue engineering

Publication date :

2018

Journal title :

Marine Drugs

ISSN :

1660-3397

Publisher :

MDPI AG

Volume :

Issue :

Pages :

350

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 07 January 2019

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