Poster (Scientific congresses and symposiums)
The GPR1/FUN34/YaaH(/SatP) members in Chlamydomonas reinhardtii, are they novel peroxisomal transporters?
Durante, Lorenzo; Lauersen, Kyle J.; Hübner, Wolfgang et al.
201818th International Conference on the Cell and Molecular Biology of Chlamydomonas
 

Files


Full Text
Durante L_poster_WDC_2018 CR_v4.pdf
Author postprint (480.49 kB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Acetate transporter; Gpr1/Fun34/yaah; Chlamydomonas reinhardtii
Abstract :
[en] The green microalga C. reinhardtii can grow heterotrophically in presence of acetate in the dark as sole carbon source, due to the anaplerotic pathway of glyoxylate cycle1 almost completely localised in the peroxisomal microbodies2 . Despite the significant advances gained on carbon metabolism, the genes involved in acetate transport are not yet identified. Recently, five functionally uncharacterized members predicted to have transporter-like structure were classified within the protein family GPR1/FUN34/YaaH(/SatP) (GFY)3, which comprises genes coding for putative carboxylic acids transporters in other microorganisms, including acetate as substrate4. In C. reinhardtii, GFY1-5 as we shown by YFP fusion protein, localise to peroxisomal membranes. In addition, we also showed that these genes present distinct expression patterns in different cultivation conditions, which correlates with associated co-expressed genes found in the databases. Therefore, although these proteins share between 78 and 97% similarity between their amino acid sequences, this might suggest non-redundant roles. To gain insight on their role, we are currently analysing insertional and knock-down mutants. Mutants for the genes GFY1, GFY2 and GFY3 were obtained from the Chlamydomonas Library Project 5. Mutants for GFY4, GFY5 and all GFY genes are presently under generation via artificial micro RNA. In conclusion, GFY members could represent different protagonists in transporting substrates in/out of peroxisomal microbodies, as putative transporters/permeases function.
Disciplines :
Microbiology
Phytobiology (plant sciences, forestry, mycology...)
Biochemistry, biophysics & molecular biology
Author, co-author :
Durante, Lorenzo ;  Université de Liège - ULiège > Département des sciences de la vie > Génétique et physiologie des microalgues
Lauersen, Kyle J.
Hübner, Wolfgang
Hanikenne, Marc  ;  Université de Liège - ULiège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale
Remacle, Claire  ;  Université de Liège - ULiège > Département des sciences de la vie > Génétique et physiologie des microalgues
Language :
English
Title :
The GPR1/FUN34/YaaH(/SatP) members in Chlamydomonas reinhardtii, are they novel peroxisomal transporters?
Publication date :
17 June 2018
Event name :
18th International Conference on the Cell and Molecular Biology of Chlamydomonas
Event date :
17-21/6/2018
Available on ORBi :
since 22 December 2018

Statistics


Number of views
76 (10 by ULiège)
Number of downloads
69 (5 by ULiège)

Bibliography


Similar publications



Contact ORBi