Article (Scientific journals)
Investigation of mycobacteria fatty acid profile using different ionization energies in GC–MS
Beccaria, M.; Franchina, Flavio; Nasir, M. et al.
2018In Analytical and Bioanalytical Chemistry, 410 (30), p. 7987-7996
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Keywords :
Bacteria; Electron impact ionization; Fatty acid methyl esters (FAMEs); Gas chromatography mass spectrometry (GC–MS); Mass spectrometry; Chromatographic analysis; Esters; Fatty acids; Gas chromatography; Ion sources; Ionization of gases; Isomers; Liquid chromatography; Volatile organic compounds; Analyte identifications; Chromatographic separations; Electron impact-ionization; Fatty acid composition; Fatty acid methyl ester; Gas chromatography-mass spectrometry; Well-established techniques; Impact ionization
Abstract :
[en] Gas chromatography (GC) coupled with electron ionization (EI) mass spectrometry (MS) is a well-established technique for the analysis of volatile and semi-volatile compounds. The main advantage is the highly repeatable fragmentation of the compounds into the ion source, generating intense and diagnostic fragmentation when the ionization is performed at 70 eV; this is considered the standard ionization condition and has been used for creating many established databases, which are of great support in the analyte identification process. However, such an intense fragmentation often causes the loss of the molecular ion or more diagnostic ions, which can be detrimental for the identification of homologous series or isomers, as for instance fatty acids. To obtain this information chemical or soft ionization can be used, but dedicated ion sources and conditions are required. In this work, we explored different ionization voltages in GC–EI–MS to preserve the intensity of the molecular ion using a conventional quadrupole MS. Twenty, 30, 50, and 70 eV were tested using a mixture of fatty acid methyl esters standards. Intensity and repeatability of the most informative ions were compared. Twenty and 70 eV were then used to analyze the fatty acid composition of six different strains of mycobacteria. Two approaches were used for elaborating the data: (1) a single average spectrum of the entire chromatogram was derived, which can be considered (in terms of concept) as a direct EI–MS analysis; (2) the actual chromatographic separation of the compounds was considered after automatic alignment. The results obtained are discussed herein. [Figure not available: see fulltext.]. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.
Disciplines :
Chemistry
Microbiology
Author, co-author :
Beccaria, M.;  Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, United States, Department for Pharmaceutical and Pharmacological Sciences, KU Leuven–University of Leuven, Leuven, 3000, Belgium
Franchina, Flavio ;  Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique, organique et biologique
Nasir, M.;  Geisel School of Medicine, Dartmouth College, Hanover, NH 03755, United States
Mellors, T.;  Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, United States
Hill, J. E.;  Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, United States, Geisel School of Medicine, Dartmouth College, Hanover, NH 03755, United States
Purcaro, Giorgia  ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie des agro-biosystèmes
Language :
English
Title :
Investigation of mycobacteria fatty acid profile using different ionization energies in GC–MS
Publication date :
2018
Journal title :
Analytical and Bioanalytical Chemistry
ISSN :
1618-2642
eISSN :
1618-2650
Publisher :
Springer Verlag
Volume :
410
Issue :
30
Pages :
7987-7996
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 26 November 2018

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