Article (Scientific journals)
Influence of bioreactor hydraulic characteristics on a Saccharomyces cerevisiae fed-batch culture: hydrodynamic modelling and scale-down investigations.
Lejeune, Annick; Delvigne, Frank; Thonart, Philippe
2010In Journal of Industrial Microbiology and Biotechnology, 37, p. 225-236
Peer Reviewed verified by ORBi
 

Files


Full Text
revised_manuscript.doc
Author postprint (142.34 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Abstract :
[en] Yeast is a widely used microorganism at the industrial level because of its biomass and metabolite production capabilities. However, due to its sensitivity to the glucose effect, problems occur during scale-up to the industrial scale. Hydrodynamic conditions are not ideal in large-scale bioreactors, and glucose concentration gradients can arise when these bioreactors are operating in fed-batch mode. We have studied the effects of such gradients in a scale-down reactor, which consists of a mixed part linked to a non-mixed part by a recirculation pump, in order to mimic the hydrodynamic conditions encountered at the large scale. During the fermentation tests in the scale-down reactor, there was a drop in both biomass yield (ratio between the biomass produced and the glucose added) and trehalose production and an increase in both fermentation time (time between inoculation and beginning of stationary phase) and ethanol production. We have developed a stochastic model which explains these effects as the result of an induction process determined mainly by the hydrodynamic conditions. The concentration profiles experienced by the microorganisms during the scale-down tests were expressed and linked to the biomass yields of the scale-down tests.
Disciplines :
Biotechnology
Author, co-author :
Lejeune, Annick ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Delvigne, Frank  ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Thonart, Philippe ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech - Biochimie et microbiologie industrielles
Language :
English
Title :
Influence of bioreactor hydraulic characteristics on a Saccharomyces cerevisiae fed-batch culture: hydrodynamic modelling and scale-down investigations.
Publication date :
2010
Journal title :
Journal of Industrial Microbiology and Biotechnology
ISSN :
1367-5435
eISSN :
1476-5535
Publisher :
Springer, Berlin, Germany
Volume :
37
Pages :
225-236
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
Available on ORBi :
since 19 September 2009

Statistics


Number of views
143 (32 by ULiège)
Number of downloads
10 (8 by ULiège)

Scopus citations®
 
10
Scopus citations®
without self-citations
7
OpenCitations
 
10

Bibliography


Similar publications



Contact ORBi