Article (Scientific journals)
Coxsackievirus B4 Infection of Murine Foetal Thymus Organ Cultures
Brilot, F.; Jaidane, H.; Geenen, Vincent et al.
2008In Journal of Medical Virology, 80 (4), p. 659-66
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Abstract :
[en] The infection of foetal thymus with coxsackievirus B4 (CV-B4) E2 has been studied ex vivo by using CD-1 mice on foetal day 14, as a ready source of organs for experimentation to investigate the hypothesis of the role of thymic viral infections in the pathogenesis of type 1 diabetes. The replication of CV-B4 E2 in murine foetal thymus organ cultures has been demonstrated by evaluating the levels of positive- and negative-stranded viral RNA in cells by using a real-time quantitative RT-PCR method and by determining titres of infectious viral particles in culture supernatants for 7 days post-infection (p.i.). Staining of tissue sections with an anti-cytokeratin antibody and haematoxylin-eosin showed that CV-B4 infection had no visible effect on cell survival and organ integrity. Cell counts in mock- and virus-infected foetal thymus organ cultures increased from day 1 through day 7, and live cell numbers were comparable in both conditions as shown by Trypan blue exclusion test and 7-amino-actinomycin D staining of thymocytes. Compared with controls on day 7 p.i., cytofluorometric analyses on cells from CV-B4 E2-infected foetal thymus organ cultures displayed a marked increase in the percentage of the most immature CD3(-)CD4(-)CD8(-) thymocytes, and a decrease in the percentage of immature CD3(-)CD4(+)CD8(+) cells, together with an increase in the percentage of mature CD3(+)CD4(+) and CD3(+)CD8(+) cells. These data show that CV-B4 E2 disturbs T-cell maturation and differentiation processes in infected murine foetal thymus organ cultures and provide evidence of a suitable system to investigate the effect of viruses in T-cell differentiation. J. Med. Virol. 80:659-666, 2008. (c) 2008 Wiley-Liss, Inc.
Disciplines :
Immunology & infectious disease
Author, co-author :
Brilot, F.
Jaidane, H.
Geenen, Vincent ;  Université de Liège - ULiège > Centre d'immunologie
Hober, D.
Language :
English
Title :
Coxsackievirus B4 Infection of Murine Foetal Thymus Organ Cultures
Publication date :
April 2008
Journal title :
Journal of Medical Virology
ISSN :
0146-6615
eISSN :
1096-9071
Publisher :
John Wiley & Sons, Hoboken, United States - New York
Volume :
80
Issue :
4
Pages :
659-66
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 18 September 2009

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