[en] Our problem with a routine LC-MS/MS system is related to the presence of an isobaric interference of cortisol from an unknown source. After cleaning and checking the proper function of the LC system, we performed an exhaustive study of all the possible contamination sources that can affect the method performance. Bovine Serum Albumin (BSA) seems the critical reactive that contained the highest amount of this interference, among other sources tested. Identity of this compound was obtained by QTOF analysis and resulted to be cortisol.
Disciplines :
Laboratory medicine & medical technology
Author, co-author :
Arias-Carnero, Benjamin
PEETERS, Stéphanie ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Laboratoire techniques séparatives et stress oxydant
DEVILLE, Marine ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Service de toxicologie
LE GOFF, Caroline ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Laboratoire techniques séparatives et stress oxydant
Cavalier, Etienne ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Fabregat Cabello, Neus ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Language :
English
Title :
Cortisol everywhere!
Publication date :
12 September 2018
Event name :
MSACL EU 2018
Event organizer :
The Association for Mass Spectrometry: Applications to the Clinical Lab (MSACL)
