An ethnobotanical survey and inhibitory effects on NLRP3 inflammasomes/Caspase-1 of herbal recipes' extracts traditionally used in Rwanda for asthma treatment.
Tomani, Jean Claude Didelot; Gainkam, Lea Olive Tchouate; Nshutiyayesu, Samuelet al.
2018 • In Journal of Ethnopharmacology, 227, p. 29-40
[en] ETHNOPHARMACOLOGICAL RELEVANCE: Respiratory diseases and asthma, in particular, are nowadays a global health problem. In Rwanda, some traditional healers claim to treat asthma with plant-based recipes, though there is no scientific proof so far. AIM OF THE STUDY: Our study aimed at evaluating the toxicity and the anti-inflammatory effect of plant recipes used in Rwanda against asthma in order to select potential candidates for further characterization of the active compounds. MATERIALS AND METHODS: Water (aqueous) and methanol-dichloromethane (organic) extracts from selected folkloric recipes were submitted for toxicity test on THP-1 derived macrophages using CellTiter-Glo Luminescent Cell Viability Assay. The evaluation of the anti-inflammatory effect of the plant extracts was carried out using the Caspase-Glo 1 Inflammasome assay on THP-1 -derived macrophages. RESULTS: Most of both organic and aqueous extract showed more than 95% of cell viability up to 200microg/ml, except for R03Cn organic extract that inhibited 25% of the cell viability. Plant extracts inhibited caspase-1 activation in THP-1 derived macrophages in a dose-dependent manner. Four extracts (R03Cn and R07Kn aqueous extracts, R10MK and R19Sz organic extracts) strongly downregulated the activation of caspase-1 (more than 70% at 50microg/ml). In general, organic extracts exhibited better caspase-1 inhibitory effects than their aqueous counterparts. CONCLUSIONS: The inhibition of inflammasome/caspase-1 is one of key mechanisms of action in asthma. Some traditional recipes are active on this mechanism and are thus strong candidates for the treatment of asthma and other inflammasome-mediated diseases. Further investigations are needed to characterize active molecules.
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Tomani, Jean Claude Didelot
Gainkam, Lea Olive Tchouate
Nshutiyayesu, Samuel
Mukazayire, Marie Jeanne
Ribeiro, Sofia Oliveira
Stevigny, Caroline
Frederich, Michel ; Université de Liège - ULiège > Département de pharmacie > Pharmacognosie
Muganga, Raymond
Souopgui, Jacob
Language :
English
Title :
An ethnobotanical survey and inhibitory effects on NLRP3 inflammasomes/Caspase-1 of herbal recipes' extracts traditionally used in Rwanda for asthma treatment.
Acosta, J., Sevilla, I., Salomón, S., Nuevas, L., Romero, A., Amaro, D., Determination of mangiferin solubility in solvents used in the biopharmaceutical industry. J. Pharm. Pharmacogn. Res. 4 (2016), 49–53.
Akinmoladun, A.C., Ibukun, E.O., Afor, E., Obuotor, E.M., Farombi, E.O., Phytochemical constituent and antioxidant activity of extract from the leaves of Ocimum gratissimum. Sci. Res. Essay 2 (2007), 163–166.
Akula, U.S., Odhav, B., In vitro 5-lipoxygenase inhibition of polyphenolic antioxidants from undomesticated plants of South Africa. J. Med. Plants Res. 2 (2008), 207–212.
Almonte-Flores, D.C., Paniagua-Castro, N., Escalona-Cardoso, G., Rosales-Castro, M., Pharmacological and genotoxic properties of polyphenolic extracts of Cedrela odorata L. and Juglans regia L. barks in rodents. Evidence-based Complement. Altern. Med., 2015, 2015, 10.1155/2015/187346.
Aruna, K., Rajeswari, P.D.R., Sankar, S.R., The effect of Oxalis corniculata extract against the behavioral changes induced by 1-methyl- 4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in mice. J. Appl. Pharm. Sci. 7 (2017), 148–153, 10.7324/JAPS.2017.70324.
Avoseh, O.N., Oyedeji, O.O., Aremu, K., Nkeh-Chungag, B.N., Songca, S.P., Oluwafemi, S.O., Oyedeji, A.O., Chemical composition and anti-inflammatory activities of the essential oils from Acacia mearnsii de Wild. Nat. Prod. Res. 29 (2015), 1184–1188, 10.1080/14786419.2014.983504.
Besnard, a.G., Togbe, D., Couillin, I., Tan, Z., Zheng, S.G., Erard, F., Le Bert, M., Quesniaux, V., Ryffel, B., Inflammasome - IL-1 - Th17 response in allergic lung inflammation. J. Mol. Cell Biol. 4 (2012), 3–10 (https://doi.org/mjr042) (pii) (10.1093/jmcb/mjr042).
Borghi, S.M., Carvalho, T.T., Staurengo-Ferrari, L., Hohmann, M.S.N., Pinge-Filho, P., Casagrande, R., Verri, W.A., Vitexin inhibits inflammatory pain in mice by targeting TRPV1, oxidative stress, and cytokines. J. Nat. Prod. 76 (2013), 1141–1146, 10.1021/np400222v.
Budai, M.M., Varga, A., Milesz, S., Tozsér, J., Benko, S., Aloe vera downregulates LPS-induced inflammatory cytokine production and expression of NLRP3 inflammasome in human macrophages. Mol. Immunol. 56 (2013), 471–479, 10.1016/j.molimm.2013.05.005.
Cao, C., Su, M., Zhou, F., Mangiferin inhibits hippocampal NLRP3 inflammasome and exerts antidepressant effects in a chronic mild stress mice model. Behav. Pharmacol. 28 (2017), 356–364, 10.1097/FBP.0000000000000305.
Chen, Y., Chen, P.D., Bao, B.H., Shan, M.Q., Zhang, K.C., Cheng, F.F., Cao, Y.D., Zhang, L., Ding, A.W., Anti-thrombotic and pro-angiogenic effects of Rubia cordifolia extract in zebrafish. J. Ethnopharmacol. 219 (2018), 152–160, 10.1016/j.jep.2017.11.005.
Chifundera, K., Contribution to the inventory of medicinal plants from the Bushi area, South Kivu Province, Democratic Republic of Congo. Fitoterapia 72 (2001), 351–368, 10.1016/S0367-326X(00)00294-X.
Cos, P., Hermans, N., De Bruyne, T., Apers, S., Sindambiwe, J.B., Witvrouw, M., De Clercq, E., Vanden Berghe, D., Pieters, L., Vlietinck, A.J., Antiviral activity of Rwandan medicinal plants against human immunodeficiency virus type-1 (HIV-1). Phytomedicine 9 (2002), 62–68, 10.1078/0944-7113-00083.
Cos, P., Hermans, N., Van Poel, B., De Bruyne, T., Apers, S., Sindambiwe, J.B., Vanden Berghe, D., Pieters, L., Vlietinck, A.J., Complement modulating activity of Rwandan medicinal plants. Phytomedicine 9 (2002), 56–61, 10.1078/0944-7113-00085.
Costa, R.S., Carneiro, T.C.B., Cerqueira-Lima, A.T., Queiroz, N.V., Alcântara-Neves, N.M., Pontes-De-Carvalho, L.C., Velozo, E.D.S., Oliveira, E.J., Figueiredo, C.A., Ocimum gratissimum Linn. and rosmarinic acid, attenuate eosinophilic airway inflammation in an experimental model of respiratory allergy to Blomia tropicalis. Int. Immunopharmacol. 13 (2012), 126–134, 10.1016/j.intimp.2012.03.012.
Crawford, A.D., Liekens, S., Kamuhabwa, A.R., Maes, J., Munck, S., Busson, R., Rozenski, J., Esguerra, C.V., de Witte, P.A.M., Zebrafish bioassay-guided natural product discovery: isolation of angiogenesis inhibitors from East African medicinal plants. PLoS One, 2011, 6, 10.1371/journal.pone.0014694.
De Wet, H., Nzama, V.N., Van Vuuren, S.F., Medicinal plants used for the treatment of sexually transmitted infections by lay people in northern Maputaland, KwaZulu-Natal Province, South Africa. South Afr. J. Bot. 78 (2012), 12–20, 10.1016/j.sajb.2011.04.002.
Devika, R., Wound healing medicinal plants-An overview. Int. J. Pharma Bio Sci. 6 (2015), B1103–B1107.
Dhimolea, E., Canakinumab. MAbs 2 (2010), 3–13, 10.4161/mabs.2.1.10328.
Domiciano, T.P., Wakita, D., Jones, H.D., Crother, T.R., Verri, W.A., Arditi, M., Shimada, K., Quercetin inhibits inflammasome activation by interfering with ASC oligomerization and prevents interleukin-1 mediated mouse vasculitis. (11 pages) Sci. Rep., 7, 2017, 10.1038/srep41539.
Dutta, A., Lahkar, M., Handique, C., Evaluation of antinociceptive activity of oxalis corniculata in streptozotocin induced diabetic rats. Int. J. Basic Clin. Pharmacol. 5 (2016), 1465–1478, 10.20959/wjpps201610-7921.
Fatiha, B.A., Ouafae, B., Souad, S., Fatima, E.H., Allal, D., Lahcen, Z., Ethnobotany study of medicinal plants used in the treatment of respiratory diseases in the Middle region of Oum Rbai. Int. J. Environ. Agric. Biotechnol. 2 (2017), 1460–1468.
Fenini, G., Contassot, E., French, L.E., Potential of IL-1, IL-18 and inflammasome inhibition for the treatment of inflammatory skin diseases. (20 pages) Front. Pharmacol., 8, 2017, 10.3389/fphar.2017.00278.
Focho, D.A., Nkeng, E.A.P., Fonge, B.A., Fongod, A.N., Muh, C.N., W.Ndam, T., Afegenui, A., Diversity of plants used to treat respiratory diseases in Tubah, northwest region, Cameroon. Afr. J. Pharm. Pharmacol. 3 (2009), 573–580.
Gao, M., Yang, J., Wang, Z., Yang, B., Kuang, H., Liu, L., Wang, L., Yang, C., Simultaneous determination of purpurin, munjistin and mollugin in rat plasma by ultra high performance liquid chromatography-tandem mass spectrometry: application to a pharmacokinetic study after oral administration of Rubia cordifolia L. extract. Molecules, 2016, 21, 10.3390/molecules21060717.
Giday, M., Asfaw, Z., Elmqvist, T., Woldu, Z., An ethnobotanical study of medicinal plants used by the Zay people in Ethiopia. J. Ethnopharmacol. 85 (2003), 43–52, 10.1016/S0378-8741(02)00359-8.
Global Asthma Network, 2014. The Global Asthma Report 2014. Auckland, New Zealand. https://doi.org/ISBN: 978-0-473-29125-9/r978-0-473-29126-6 (ELECTRONIC).
Gong, T., Wang, X., Yang, Y., Yan, Y., Yu, C., Zhou, R., Jiang, W., Plant lectins activate the NLRP3 inflammasome to promote inflammatory disorders. J. Immunol. 198 (2017), 2082–2092, 10.4049/jimmunol.1600145.
Hirsch, C., 2014. Culturing and differentiating THP-1 cells.
Huang, F.-J., Hsuuw, Y.-D., Chan, W.-H., Characterization of Apoptosis induced by emodin and related regulatory mechanisms in human neuroblastoma cells. Int. J. Mol. Sci. 14 (2013), 20139–20156, 10.3390/ijms141020139.
Ikarashi, N., Toda, T., Hatakeyama, Y., Kusunoki, Y., Kon, R., Mizukami, N., Kaneko, M., Ogawa, S., Sugiyama, K., Anti-hypertensive effects of acacia polyphenol in spontaneously hypertensive rats. Int. J. Mol. Sci., 2018, 19, 10.3390/ijms19030700.
Juliana, C., Fernandes-Alnemri, T., Kang, S., Farias, A., Qin, F., Alnemri, E.S., Non-transcriptional priming and deubiquitination regulate NLRP3 inflammasome activation. J. Biol. Chem. 287 (2012), 36617–36622, 10.1074/jbc.M112.407130.
Kamagaju, L., Bizuru, E., Minani, V., Morandini, R., Stévigny, C., Ghanem, G., Duez, P., An ethnobotanical survey of medicinal plants used in Rwanda for voluntary depigmentation. J. Ethnopharmacol. 150 (2013), 708–717, 10.1016/j.jep.2013.09.031.
Kamatenesi-Mugisha, M., Oryem-Origa, H., Medicinal plants used to induce labour during childbirth in western Uganda. J. Ethnopharmacol. 109 (2007), 1–9, 10.1016/j.jep.2006.06.011.
Kang, M., Yun, S., Won, J., Rosmarinic acid inhibits Ca. Cell 101 (2003), 3534–3542, 10.1182/blood-2002-07-1992.Supported.
Kassim, M., Achoui, M., Mustafa, M.R., Mohd, M.A., Yusoff, K.M., Ellagic acid, phenolic acids, and flavonoids in Malaysian honey extracts demonstrate in vitro anti-inflammatory activity. Nutr. Res. 30 (2010), 650–659, 10.1016/j.nutres.2010.08.008.
Kayani, S., Ahmad, M., Zafar, M., Sultana, S., Khan, M.P.Z., Ashraf, M.A., Hussain, J., Yaseen, G., Ethnobotanical uses of medicinal plants for respiratory disorders among the inhabitants of Gallies - Abbottabad, Northern Pakistan. J. Ethnopharmacol. 156 (2014), 47–60, 10.1016/j.jep.2014.08.005.
Kipassa, N.T., Iwagawa, T., Okamura, H., Doe, M., Morimoto, Y., Nakatani, M., Limonoids from the stem bark of Cedrela odorata. Phytochemistry 69 (2008), 1782–1787, 10.1016/j.phytochem.2007.12.015.
Lin, X., Liu, M., 2017. Pharmacological Use of NLRP3 Inflammasome Inhibitors: Novel Intervention Strategies in Diabetes-Associated Vascular Complications 2, 35–36. https://doi.org/10.1038/nm.2279.2.
MacKinnon, S., Durst, T., Arnason, J.T., Angerhofer, C., Pezzuto, J., Sanchez-Vindas, P.E., Poveda, L.J., Gbeassor, M., Antimalarial activity of tropical Meliaceae extracts and gedunin derivatives. J. Nat. Prod. 60 (1997), 336–341, 10.1021/np9605394.
Madouri, F., Guillou, N., Fauconnier, L., Marchiol, T., Rouxel, N., Chenuet, P., Ledru, A., Apetoh, L., Ghiringhelli, F., Chamaillard, M., Zheng, S.G., Trovero, F., Quesniaux, V.F.J., Ryffel, B., Togbe, D., Caspase-1 activation by NLRP3 inflammasome dampens IL-33-dependent house dust mite-induced allergic lung inflammation. J. Mol. Cell Biol. 7 (2015), 351–365, 10.1093/jmcb/mjv012.
Makare, N., Bodhankar, S., Rangari, V., Immunomodulatory activity of alcoholic extract of Mangifera indica L. in mice. J. Ethnopharmacol. 78 (2001), 133–137, 10.1016/S0378-8741(01)00326-9.
Marciniuk, D., Ferkol, T., Nana, A., Oca, M.M., de, Rabe, K., Billo, N., Zar, H., Respiratory Diseases in the World. Realities of Today – Opportunities for Tomorrow. 2013, European Respiratory Society, Sheffield/UK.
Matu, E.N., Van Staden, J., Antibacterial and anti-inflammatory activities of some plants used for medicinal purposes in Kenya. J. Ethnopharmacol. 87 (2003), 35–41, 10.1016/S0378-8741(03)00107-7.
Moshi, M.J., Otieno, D.F., Mbabazi, P.K., Weisheit, A., Ethnomedicine of the Kagera Region, north western Tanzania. Part 2: the medicinal plants used in Katoro Ward, Bukoba District. (17 pages) J. Ethnobiol. Ethnomed., 6, 2010, 10.1186/1746-4269-6-19.
Mukazayire, M.J., Minani, V., Ruffo, C.K., Bizuru, E., Stévigny, C., Duez, P., Traditional phytotherapy remedies used in Southern Rwanda for the treatment of liver diseases. J. Ethnopharmacol. 138 (2011), 415–431, 10.1016/j.jep.2011.09.025.
Musafiri, S., Van Meerbeeck, J., Musango, L., Brusselle, G., Joos, G., Seminega, B., Rutayisire, C., Prevalence of atopy, asthma and COPD in an urban and a rural area of an African country. Respir. Med. 105 (2011), 1596–1605, 10.1016/j.rmed.2011.06.013.
Mzindle, N.B., Properties of Selected South African medicinal plants Submitted in Fulfillment for the Degree of Master of Applied Sciences. 2017, Durban University of Technology.
Nachbar, M.S., Oppenheim, J.D., Lectins in the United States diet: a survey of lectins in commonly consumed foods and a review of the literature. Am. J. Clin. Nutr. 33 (1980), 2338–2345, 10.1093/ajcn/33.11.2338.
Ngezahayo, J., Havyarimana, F., Hari, L., Stévigny, C., Duez, P., Medicinal plants used by Burundian traditional healers for the treatment of microbial diseases. J. Ethnopharmacol. 173 (2015), 338–351, 10.1016/j.jep.2015.07.028.
Ogawa, S., Matsumae, T., Kataoka, T., Yazaki, Y., Yamaguchi, H., Effect of acacia polyphenol on glucose homeostasis in subjects with impaired glucose tolerance: a randomized multicenter feeding trial. Exp. Ther. Med. 5 (2013), 1566–1572, 10.3892/etm.2013.1029.
Okello, J., Ssegawa, P., Medicinal plants used by communities of Ngai Subcounty, Apac District, northern Ugand. Afr. J. Ecol. 45 (2007), 76–83, 10.1111/j.1365-2028.2007.00742.x.
Okorie, D.A., Taylor, D.A.H., Extractives from the seed of Cedrela odorata L. Phytochemistry 7 (1968), 1683–1686, 10.1016/S0031-9422(00)88625-2.
Padayachee, B., Immunomodulatory activities of non-commercialized leafy vegetables in KwaZulu-Natal. 2012, Durban University of Technology, South Africa.
Pan, S.Y., Zhou, S.F., Gao, S.H., Yu, Z.L., Zhang, S.F., Tang, M.K., Sun, J.N., Ma, D.L., Han, Y.F., Fong, W.F., Ko, K.M., New perspectives on how to discover drugs from herbal medicines: CAM'S outstanding contribution to modern therapeutics. (25 pages) Evid.-Based Complement. Altern. Med., 2013, 2013, 10.1155/2013/627375.
Pinkerton, J.W., Kim, R.Y., Robertson, A.A.B., Hirota, J.A., Wood, L.G., Knight, D.A., Cooper, M.A., O'Neill, L.A.J., Horvat, J.C., Hansbro, P.M., Inflammasomes in the lung. Mol. Immunol. 86 (2017), 44–55, 10.1016/j.molimm.2017.01.014.
Prati, M., Biganzoli, E., Boracchi, P., Tesauro, M., Monetti, C., Bernardini, G., Ecotoxicological soil evaluation by FETAX. Chemosphere 41 (2000), 1621–1628, 10.1016/S0045-6535(00)00034-5.
Rabelo, M., Souza, E.P., Soares, P.M.G., Miranda, A.V., Matos, F.J.A., Criddle, D.N., Antinociceptive properties of the essential oil of Ocimum gratissimum L. (Labiatae) in mice. Braz. J. Med. Biol. Res. 36 (2003), 521–524, 10.1590/S0100-879X2003000400016.
Rashed, K., Anthonissen, R., Cappoen, D., Verschaeve, L., Phytochemical composition and potential genotoxic effects of important Egyptian medicinal plants. Pharmacogn. Commun. 5 (2015), 207–216, 10.5530/pc.2015.3.6.
Rashed, K.R., Antioxidant potential of Cedrela odorata stems extracts and Bio active Phytoconstituents. Hygeiajournal Drugs Med. 6 (2014), 25–30, 10.15254/H.J.D.Med.6.2014.119.
Sanders, B., Ray, A.M., Goldberg, S., Clark, T., Mcdaniel, H.R., Atlas, S.E., Konefal, J., Lages, L.C., Lopez, J., Rasul, A., Tiozzo, E., Woolger, J.M., Lewis, J.E., Anti-cancer effects of aloe-emodin: a systematic review. J. Clin. Transl. Res., 3, 2017 (xxx–xxx).
Sbai-Jouilil, H., Fadli, A., El Hafian, M., El Ayad, R., Benharbit, O., Zidane, L., Floristic and ethnobotanical study of medicinal plants used in the treatment of respiratory diseases in Seksaoua region (Western high Moroccan atlas). Annu. Res. Rev. Biol. 17 (2017), 1–10, 10.9734/ARRB/2017/36526.
Sharma, R.A., Kumari, A., Phytochemistry, pharmacology and therapeutic application of Oxalis corniculata Linn. - A review. Int. J. Pharm. Pharm. Sci. 6 (2014), 6–12.
Silva, A.C.O., Santana, E.F., Saraiva, A.M., Coutinho, F.N., Castro, R.H.A., Pisciottano, M.N.C., Amorim, E.L.C., Albuquerque, U.P., Which approach is more effective in the selection of plants with antimicrobial activity? Evidence-based Complement. (9 pages) Altern. Med., 2013, 2013, 10.1155/2013/308980.
Silva, E.M., Souza, J.N.S., Rogez, H., Rees, J.F., Larondelle, Y., Antioxidant activities and polyphenolic contents of fifteen selected plant species from the Amazonian region. Food Chem. 101 (2006), 1012–1018, 10.1016/j.foodchem.2006.02.055.
Ssegawa, P., Kasenene, J.M., Medicinal plant diversity and uses in the Sango bay area, Southern Uganda. J. Ethnopharmacol. 113 (2007), 521–540, 10.1016/j.jep.2007.07.014.
Tabuti, J.R.S., Kukunda, C.B., Waako, P.J., Medicinal plants used by traditional medicine practitioners in the treatment of tuberculosis and related ailments in Uganda. J. Ethnopharmacol. 127 (2010), 130–136, 10.1016/j.jep.2009.09.035.
Takano, H., Osakabe, N., Sanbongi, C., Yanagisawa, R., Inoue, K.-I., Yasuda, A., Natsume, M., Baba, S., Ichiishi, E.-I., Yoshikawa, T., Extract of Perilla frutescens Enriched for rosmarinic acid, a polyphenolic phytochemical, inhibits seasonal allergic rhinoconjunctivitis in humans. Exp. Biol. Med. 229 (2004), 247–254, 10.1258/ebm.2011.010287.
Tanko, Y., Magaji, G.M., Yerima, M., Magaji, R.A., Mohammed, A., Anti-nociceptive and anti-inflammatory activities of aqueous leaves extract of Ocimum gratissimum (Labiate) in rodents. Afr. J. Tradit. Complement. Altern. Med. 5 (2008), 141–146.
Telefo, P.B., Lienou, L.L., Yemele, M.D., Lemfack, M.C., Mouokeu, C., Goka, C.S., Tagne, S.R., Moundipa, F.P., Ethnopharmacological survey of plants used for the treatment of female infertility in Baham, Cameroon. J. Ethnopharmacol. 136 (2011), 178–187, 10.1016/j.jep.2011.04.036.
Togola, A., Diallo, D., Dembélé S., Barsett, H., Paulsen, B.S., Ethnopharmacological survey of different uses of seven medicinal plants from Mali, (West Africa) in the regions Doila, Kolokani and Siby. (9 pages) J. Ethnobiol. Ethnomed., 1, 2005, 10.1186/1746-4269-1-7.
Tongco, M.D.C., Purposive sampling as a tool for informant selection. Ethnobot. Res. Appl. 5 (2007), 147–158, 10.17348/era.5.0.147-158.
Troupin, G., 1982. Flore des plantes ligneuses du Rwanda. Public. I.N.R.S., Butare-Rwanda.
Tugume, P., Kakudidi, E.K., Buyinza, M., Namaalwa, J., Kamatenesi, M., Mucunguzi, P., Kalema, J., Ethnobotanical survey of medicinal plant species used by communities around Mabira Central Forest Reserve, Uganda. (28 pages) J. Ethnobiol. Ethnomed., 12, 2016, 10.1186/s13002-015-0077-4.
Verma, A., Kumar, B., Alam, P., Singh, V., Gupta, S.K., Rubia cordifolia - a review on pharmaconosy and phytochemistry. Int. J. Pharm. Sci. Res. 7 (2016), 2720–2731.
Wanderer, A.A., Interleukin-1beta targeted therapy in severe persistent asthma (SPA) and chronic obstructive pulmonary disease (COPD): proposed similarities between biphasic pathobiology of SPA/COPD and ischemia-reperfusion injury. Isr. Med. Assoc. J. 10 (2008), 837–842.
World Health Organization, 2010. Global status report on noncommunicable diseases 2010, World Health. https://doi.org/978 92 4 156422 9.
Xiao, J., Liu, Y., Xing, F., Leung, T.M., Liong, E.C., Tipoe, G.L., Bee's honey attenuates non-alcoholic steatohepatitis-induced hepatic injury through the regulation of thioredoxin-interacting protein–NLRP3 inflammasome pathway. Eur. J. Nutr. 55 (2016), 1465–1477, 10.1007/s00394-015-0964-4.
Xiong, J., Grace, M.H., Esposito, D., Komarnytsky, S., Wang, F., Lila, M.A., Polyphenols isolated from Acacia mearnsii bark with anti-inflammatory and carbolytic enzyme inhibitory activities. Chin. J. Nat. Med. 15 (2017), 816–824, 10.1016/S1875-5364(18)30015-3.
Yemele, M.D., Telefo, P.B., Lienou, L.L., Tagne, S.R., Fodouop, C.S.P., Goka, C.S., Lemfack, M.C., Moundipa, F.P., Ethnobotanical survey of medicinal plants used for pregnant womens health conditions in Menoua division-West Cameroon. J. Ethnopharmacol. 160 (2015), 14–31, 10.1016/j.jep.2014.11.017.
Yeo, D., N'Guessan, J.D., Sea, T., Coulibaly, Y.A., Djaman, A.J., Tako, N.A., Yavo, J.C., Guede-Guina, F., Evaluation de l'activité antiasthmatique et antitussive de Combretum molle, plante médicinale de la pharmacopée ivoirienne. Phytothérapie 6 (2008), 348–349, 10.1007/s10298-008-0347-6.
