Consensus guidelines for the use and interpretation of angiogenesis assays.

Angiogenesis; Aortic ring; Chorioallantoic membrane (CAM); Corneal angiogenesis; Endothelial cell migration; Hindlimb ischemia; Intussusceptive angiogenesis; Microfluidic; Myocardial angiogenesis; Plug assay; Proliferation; Recombinant proteins; Retinal vasculature; Tip cells; Vascular network; Vessel co-option; Zebrafish

Abstract :

[en] The formation of new blood vessels, or angiogenesis, is a complex process that plays important roles in growth and development, tissue and organ regeneration, as well as numerous pathological conditions. Angiogenesis undergoes multiple discrete steps that can be individually evaluated and quantified by a large number of bioassays. These independent assessments hold advantages but also have limitations. This article describes in vivo, ex vivo, and in vitro bioassays that are available for the evaluation of angiogenesis and highlights critical aspects that are relevant for their execution and proper interpretation. As such, this collaborative work is the first edition of consensus guidelines on angiogenesis bioassays to serve for current and future reference.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Nowak-Sliwinska, Patrycja

Alitalo, Kari

Allen, Elizabeth

Anisimov, Andrey

Aplin, Alfred C.

Auerbach, Robert

Augustin, Hellmut G.

Bates, David O.

van Beijnum, Judy R.

Bender, R. Hugh F.

More authors (76 more)

Language :

English

Title :

Consensus guidelines for the use and interpretation of angiogenesis assays.

Publication date :

2018

Journal title :

Angiogenesis

ISSN :

0969-6970

eISSN :

1573-7209

Publisher :

Kluwer Academic Publishers, Netherlands

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 24 August 2018

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427

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349

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359

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