Tools for the determination of population heterogeneity caused by inhomogeneous cultivation conditions

Biological noise; Gradients; Microbial population; Population heterogeneity; Process analytical tools; Batch data processing; Optimization; Process control; Microbial populations; Process analytical tool; Cultivation; Review; Bacteriological Techniques; Bioreactors; Flow Cytometry

Abstract :

[en] Population heterogeneity among a microbial culture can occur in various stages of a bioprocess. When a nutrient-limited fed-batch process is applied in the large scale, gradient formation (e.g. at the substrate concentration or the pH-value) can have a tremendous impact on the process performance. It might also have an impact on population heterogeneity, as the cells are opposed to stressful conditions that is an oscillating environment, when they pass the different zones evolved in the liquid phase. Nevertheless, the question whether these conditions support heterogeneity of a culture has not been clearly answered so far. Furthermore, if such a heterogeneity affects product formation, the usual analysis tools, which do not rely on a single-cell basis, certainly do not gain sufficiently suitable information for process optimization. This overview on the one hand provides information about the contribution of an oscillating environment to the formation of heterogeneities within a population and on the other hand a summary of tools, which can be used to investigate physiologic and morphologic heterogeneity during process development, scale up and production. If these techniques are considered, the identification of targets for accelerated process optimization on a single-cell basis becomes easier and faster. © 2017 Elsevier B.V.

Disciplines :

Biotechnology

Author, co-author :

Lemoine, A.; Chair of Bioprocess Engineering, TU Berlin, Germany

Delvigne, Frank ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies

Bockisch, A.; Chair of Bioprocess Engineering, TU Berlin, Germany

Neubauer, P.; Chair of Bioprocess Engineering, TU Berlin, Germany

Junne, S.; Chair of Bioprocess Engineering, TU Berlin, Germany

Language :

English

Title :

Tools for the determination of population heterogeneity caused by inhomogeneous cultivation conditions

Publication date :

2017

Journal title :

Journal of Biotechnology

ISSN :

0168-1656

eISSN :

1873-4863

Publisher :

Elsevier B.V.

Volume :

251

Pages :

84-93

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 02 July 2018

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