Bioprocess scale-up/down as integrative enabling technology: from fluid mechanics to systems biology and beyond

[en] Efficient optimization of microbial processes is a critical issue for achieving a number of sustainable development goals, considering the impact of microbial biotechnology in agrofood, environment, biopharmaceutical and chemical industries. Many of these applications require scale-up after proof of concept. However, the behaviour of microbial systems remains unpredictable (at least partially) when shifting from laboratory-scale to industrial conditions. The need for robust microbial systems is thus highly needed in this context, as well as a better understanding of the interactions between fluid mechanics and cell physiology. For that purpose, a full scale-up/down computational framework is already available. This framework links computational fluid dynamics (CFD), metabolic flux analysis and agent-based modelling (ABM) for a better understanding of the cell lifelines in a heterogeneous environment. Ultimately, this framework can be used for the design of scale-down simulators and/or metabolically engineered cells able to cope with environmental fluctuations typically found in large-scale bioreactors. However, this framework still needs some refinements, such as a better integration of gas–liquid flows in CFD, and taking into account intrinsic biological noise in ABM. © 2017 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.

Disciplines :

Biotechnology

Author, co-author :

Delvigne, Frank ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies

Takors, R.; Institute of Biochemical Engineering, University of Stuttgart, Stuttgart, Germany

Mudde, R.; Transport Phenomena Section, Department of Chemical Engineering, Delft University of Technology, Delft, Netherlands

van Gulik, W.; Department of Biotechnology, Delft University of Technology, Delft, Netherlands

Noorman, H.; Department of Biotechnology, Delft University of Technology, Delft, Netherlands, DSM Biotechnology Center, Delft, Netherlands

Language :

English

Title :

Bioprocess scale-up/down as integrative enabling technology: from fluid mechanics to systems biology and beyond

Publication date :

2017

Journal title :

Microbial Biotechnology

ISSN :

1751-7915

eISSN :

1751-7907

Publisher :

John Wiley and Sons Ltd

Volume :

Issue :

Pages :

1267-1274

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 02 July 2018

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Bibliography

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