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Abstract :
[en] Canine idiopathic pulmonary fibrosis (CIPF) mimics human IPF and affects old dogs from one breed: the West Highland white terrier (WHWT). In human IPF, lung microbiota is suspected to be associated with disease pathogenesis and might serve as a therapeutic target.
The aim of the study was to characterize microbial alteration associated with breed, environment and CIPF disease. Bronchoalveolar lavage fluid was sampled from 4 groups of dogs: client-owned WHWTs affected with CIPF from Belgium-BE (n=7, 11.6y), healthy client-owned BE-WHWTs (n=5, 11.2y), healthy client-owned WHWTs from Finland-FI (n=5, 11.0y) and healthy experimental BE-beagle dogs (n=6, 8.8y). Metagenetic analysis was performed on V1-V3 hypervariable region of 16S rDNA after total bacterial DNA extraction and sequencing on a MiSeq Illumina sequencer. Data were compared between healthy BE-WHWTs and BE-beagles, healthy BE-WHWTs and FI-WHWTs, and healthy BE-WHWTs and CIPF BE-WHWTs.
Data analyses demonstrated that the same phyla (Proteobacteria, Bacteroidetes, Actinobacteria and Firmicutes) predominated in all groups. Significant differences (p<005) were mainly noted for species with relative abundance of less than 1%, except for Micrococcus luteus, higher in FI-WHWTs (7.4%) compared to BE-WHWTs (0%) and for Pseudomonas JF766687 and EU373411, Serratia and Hydrogenophilus spp, higher in BE-beagles (15.3, 8.1, 5.0 and 1.5%) compared with BE-WHWTs (0.3, 0.3, 0, 0.1%, respectively).
Results indicate that further analysis of the impact of breed or environment and inclusion of larger amounts of dogs are needed before investigation of lung microbiota as a biomarker or therapeutic target in CIPF.
