Keywords :
Apoptosis/genetics; Cell Adhesion/genetics; Cell Line, Tumor; Ceramides/pharmacology; Colonic Neoplasms/chemistry/genetics/pathology; Computational Biology; Electrophoresis, Gel, Two-Dimensional; Gene Expression Regulation, Neoplastic/drug effects; Humans; Neoplasm Proteins/analysis; Proteomics/methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Stress, Physiological
Abstract :
[en] Comparative cancer cell proteome analysis is a strategy to study the implication of ceramides in the transmission of stress signals. To better understand the mechanisms by which ceramide regulate some physiological or pathological events and the response to the pharmacological treatment of cancer, we performed a differential analysis of the proteome of HCT-116 (human colon carcinoma) cells in response to these substances. We first established the first 2-dimensional map of the HCT-116 proteome. Then, HCT116 cell proteome treated or not with C6-ceramide have been compared using two-dimensional electrophoresis, matrix-assisted laser desorption/ionization-mass spectrometry and bioinformatic (genomic databases). 2-DE gel analysis revealed more than fourty proteins that were differentially expressed in control cells and cells treated with ceramide. Among them, we confirmed the differential expression of proteins involved in apoptosis and cell adhesion.
Funding text :
M.F. and M.-P.M. are Senior Research Assistants at the National Fund for Scientific Research (FNRS, Belgium). J.P. is Research Director at FNRS. We also thank the “Centre Anti-Cancéreux” (Liège, Belgium) and the National Fund for Scientific research (FNRS) for their financial support. The Proteomics facility used in this study was funded by the European Community (FEDER), the Région Nord-Pas de Calais (France), the CNRS, the Génopole of Lille and the Université des Sciences et Technologies de Lille.
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