Simultaneous three-dimensional visualization of mineralized and soft skeletal tissues by a novel microCT contrast agent with polyoxometalate structure.
Kerckhofs, Greet; Stegen, Steve; van Gastel, Nicket al.
3D virtual histology; Bone marrow adipocytes; Bone marrow vasculature; Contrast-enhanced computed tomography; Polyoxometalates
Abstract :
[en] Biological tissues have a complex and heterogeneous 3D structure, which is only partially revealed by standard histomorphometry in 2D. We here present a novel chemical compound for contrast-enhanced microfocus computed tomography (CE-CT), a Hafnium-based Wells-Dawson polyoxometalate (Hf-POM), which allows simultaneous 3D visualization of mineralized and non-mineralized skeletal tissues, such as mineralized bone and bone marrow vasculature and adipocytes. We validated the novel contrast agent, which has a neutral pH in solution, by detailed comparison with (immuno)histology on murine long bones as blueprint, and showed that Hf-POM-based CE-CT can be used for virtual 3D histology. Furthermore, we quantified the 3D structure of the different skeletal tissues, as well as their spatial relation to each other, during aging and diet-induced obesity. We discovered, based on a single CE-CT dataset per sample, clear differences between the groups in bone structure, vascular network organization, characteristics of the adipose tissue and proximity of the different tissues to each other. These findings highlight the complementarity and added value of Hf-POM-based CE-CT compared to standard histomorphometry. As this novel technology provides a detailed 3D simultaneous representation of the structural organization of mineralized bone and bone marrow vasculature and adipose tissue, it will enable to improve insight in the interactions between these three tissues in several bone pathologies and to evaluate the in vivo performance of biomaterials for skeletal regeneration.
Disciplines :
Mechanical engineering
Author, co-author :
Kerckhofs, Greet
Stegen, Steve
van Gastel, Nick
Sap, Annelies
Falgayrac, Guillaume
Penel, Guillaume
Durand, Marjorie
Luyten, Frank P.
Geris, Liesbet ; Université de Liège - ULiège > Département d'aérospatiale et mécanique > Génie biomécanique
Vandamme, Katleen
Parac-Vogt, Tatjana
Carmeliet, Geert
Language :
English
Title :
Simultaneous three-dimensional visualization of mineralized and soft skeletal tissues by a novel microCT contrast agent with polyoxometalate structure.
Publication date :
2018
Journal title :
Biomaterials
ISSN :
0142-9612
eISSN :
1878-5905
Publisher :
Elsevier, United Kingdom
Volume :
159
Pages :
1-12
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Copyright (c) 2018 Elsevier Ltd. All rights reserved.
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