[en] A method was developed for the quantitative analysis of six water-soluble vitamins (thiamine, nicotinamide, riboflavine, pyridoxine, ascorbic acid and pantothenic acid) in a pharmaceutical formulation, using free solution capillary zone electrophoresis (CZE) in uncoated fused silica capillaries and UV detection. The influence of different parameters, such as the nature of the buffer anionic component and buffer concentration on the CZE separation of vitamins was investigated using four vitamins of the B group as model compounds. A good compromise between resolution, analysis time and analyte stability was obtained by use of a 50 mM borax buffer of pH 8.5. This CZE method was found to be very useful for the separation of more complex samples, a mixture of ten water-soluble vitamins being completely resolved in about 10 min. However, cyanocobalamine could not be separated from nicotinamide in this CZE system, the two compounds being in uncharged form at the pH used. These two compounds could easily be resolved by micellar electrokinetic chromatography (MEKC), the anionic surfactant dodecylsulfate being added to the running buffer at 25 mM concentration. In the pharmaceutical formulation, some excipients were found to be adsorbed to the capillary surface, giving rise to a progressive decrease of the electroosmotic flow and consequently to a simultaneous increase of analyte migration times. A capillary wash with sodium hydroxide had to be made between successive runs in order to minimize these effects. Good results with respect to linearity, precision and accuracy were obtained in the concentration range studied for the six vitamins, using nicotinic acid as internal standard.
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Fotsing, Lucas ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Fillet, Marianne ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.
Bibliography
S. Fujiwara, S. Iwase and S. Honda, J. Chromatogr., 447 (1980) 133-140.
H. Nishi, N. Tsumagari, T. Kakimoto and S. Terabe, J. Chromatogr., 465 (1989) 331-343.
S. Kobayashi, T. Ueda and M. Kikumoto, J. Chromatogr., 480 (1989) 179-184.
G. Dinelli and A. bonetti, Electrophoresis, 15 (1994) 1147-1150.
S. Boonkerd, M.R. Detaevernier and Y. Michotte, J. Chromatogr. A, 670 (1994) 209-214.
U. Jegle, J. Chromatogr. A, 652 (1993) 495-501.
J. Schiewe, Y. Mrestani and R. Neubert, J. Chromatogr. A, 717 (1995) 255-259.
R. Huopalathi and J. Sunell, J. Chromatogr., 636 (1993) 133-135.
M. Chiari, M. Nesi, G. Carrea and P.G. Righetti, J. Chromatogr. A, 645 (1993) 197-200.
D. Belder and G. Schomburg, J. High. Res. Chromatogr., 15 (1992) 686-693.
H.J.P. Slevert and A.C.J.H. Drouen, Diode array detection in HPLC, Marcel Dekker, NY, 1993, p. 51.
D.L. Massart, B.G.M. Vandeginste, S.N. Deming, Y. Michotte and L. Kaufman, Chemometrics: a textbook, Elsevier, Amsterdam, 1988, p. 75.
J. Caporal-Gautier, J.M. Nivet, P. Algranti, M. Cuilloteau, M. Histe, M. Lallier, J.J. N'Guyen-Huu, R. Russotto, S.T.P. Pharma Prat., 2 (1992) 202-226.
C. Hartmann, Analusis, 22 (1994) M19-M20.
Similar publications
Sorry the service is unavailable at the moment. Please try again later.
This website uses cookies to improve user experience. Read more
Save & Close
Accept all
Decline all
Show detailsHide details
Cookie declaration
About cookies
Strictly necessary
Performance
Strictly necessary cookies allow core website functionality such as user login and account management. The website cannot be used properly without strictly necessary cookies.
This cookie is used by Cookie-Script.com service to remember visitor cookie consent preferences. It is necessary for Cookie-Script.com cookie banner to work properly.
Performance cookies are used to see how visitors use the website, eg. analytics cookies. Those cookies cannot be used to directly identify a certain visitor.
Used to store the attribution information, the referrer initially used to visit the website
Cookies are small text files that are placed on your computer by websites that you visit. Websites use cookies to help users navigate efficiently and perform certain functions. Cookies that are required for the website to operate properly are allowed to be set without your permission. All other cookies need to be approved before they can be set in the browser.
You can change your consent to cookie usage at any time on our Privacy Policy page.