Article (Scientific journals)
Detection of Hermetia illucens by real-time PCR
Marien, A; Debode, F; Aerts, C et al.
2018In Journal of Insects as Food and Feed
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Keywords :
insect, , black soldier fly, detection,; real-time PCR; Hermetia illucens
Abstract :
[en] Insects are rich in proteins and could be an alternative source of macronutrients to feed animals and humans. Over the past few years, numerous companies have started producing insects for feed purposes. In Europe, the processed animal proteins obtained from seven insect species have been authorised for aquaculture by Commission Regulation (EU) 2017/893 since 1 July 2017. Methods of authentication are required to check the conformity of the products. In this study, we propose a real-time PCR method for the specific detection of the black soldier fly (Hermetia illucens L.), one of the most widely used insects for feed production. The developed PCR assays amplify a 67 bp fragment based on the mitochondrial COX3 gene coding for subunit 3 of the cytochrome c oxidase. The qualitative method was tested according to several performance criteria. The specificity was tested against 51 insect species. The specificity was also checked against plant species and other animal species such as crustaceans, mammals and birds. The sensitivity, efficiency and robustness of the PCR test were successfully tested. The applicability of the test was proven through the analysis of real-life processed samples (industrial meals) of H. illucens.
Disciplines :
Entomology & pest control
Author, co-author :
Marien, A;  Walloon Agricultural Research Center (CRA-W), > Unit Traceability and Authentication
Debode, F;  Walloon Agricultural Research Center (CRA-W), > Unit Traceability and Authentication
Aerts, C;  Walloon Agricultural Research Center (CRA-W), > Unit Traceability and Authentication
Ancien, C;  Walloon Agricultural Research Center (CRA-W), > Unit Traceability and Authentication
Francis, Frédéric  ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Gestion durable des bio-agresseurs
Berben, G;  Walloon Agricultural Research Center (CRA-W), > Unit Traceability and Authentication,
Language :
English
Title :
Detection of Hermetia illucens by real-time PCR
Publication date :
2018
Journal title :
Journal of Insects as Food and Feed
eISSN :
2352-4588
Publisher :
Wageningen Academic, Wageningen, Netherlands
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 13 June 2018

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