The wheat R2R3-MYB transcription factor TaRIM1 participates in resistance response against the pathogen Rhizoctonia cerealis infection through regulating defense genes

Shan, Tianlei; Rong, Wei; Xu, Huijun; Du, Lipu; Liu, Xin; Zhang, Zengyan

doi:10.1038/srep28777

Article (Scientific journals)

The wheat R2R3-MYB transcription factor TaRIM1 participates in resistance response against the pathogen Rhizoctonia cerealis infection through regulating defense genes

Shan, Tianlei; Rong, Wei; Xu, Huijun et al.

2016 • In Scientific Reports

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https://hdl.handle.net/2268/223890

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10.1038/srep28777

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27364458

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Abstract :

[en] The necrotrophic fungus Rhizoctonia cerealis is a major pathogen of sharp eyespot that is a devastating disease of wheat (Triticum aestivum). Little is known about roles of MYB genes in wheat defense response to R. cerealis. In this study, TaRIM1, a R. cerealis-induced wheat MYB gene, was identied by transcriptome analysis, then cloned from resistant wheat CI12633, and its function and preliminary mechanism were studied. Sequence analysis showed that TaRIM1 encodes a R2R3-MYB transcription factor with transcription-activation activity. The molecular-biological assays revealed that the TaRIM1 protein localizes to nuclear and can bind to ve MYB-binding site cis-elements. Functional dissection results showed that following R. cerealis inoculation, TaRIM1 silencing impaired the resistance of wheat CI12633, whereas TaRIM1 overexpression signi cantly increased resistance of transgenic wheat compared with susceptible recipient. TaRIM1 positively regulated the expression of ve defense genes (Defensin, PR10, PR17c, nsLTP1, and chitinase1) possibly through binding to MYB-binding sites in their promoters. These results suggest that the R2R3-MYB transcription factor TaRIM1 positively regulates resistance response to R. cerealis infection through modulating the expression of a range of defense genes, and that TaRIM1 is a candidate gene to improve sharp eyespot resistance in wheat.

Disciplines :

Biotechnology

Author, co-author :

Shan, Tianlei

Rong, Wei ; Université de Liège - ULiège > Doct. sc. agro. & ingé. biol. (Paysage)

Xu, Huijun

Du, Lipu

Liu, Xin

Zhang, Zengyan

Language :

English

Title :

The wheat R2R3-MYB transcription factor TaRIM1 participates in resistance response against the pathogen Rhizoctonia cerealis infection through regulating defense genes

Publication date :

2016

Journal title :

Scientific Reports

eISSN :

2045-2322

Publisher :

Nature Publishing Group, United Kingdom

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 28 May 2018

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