[en] Films based on xylose and peanut protein isolate (PPI) were prepared by solution casting. The effects of
xylose content (1%, 2%, 5%, 10%, 20% PPI, w/w) on the structure and mechanical properties of PPI–
xylose (PPI–X) film were systematically investigated. The xylose-modified PPI was firstly prepared by
crosslinking and modifying of peanut isolated protein with xylose. The degree of glycosylation and
surface hydrophobicity significantly increased from 5.6% to 10.9% and from 130.6 to 370.0, respectively,
with the increase of the xylose content. Maillard reactions occurring between PPI and xylose were
confirmed by Fourier transform infrared spectroscopy. Then the xylose-modified PPI was developed into
films. PPI–X films showed markedly increased tensile strength (increased by 77%) and elongation
(increased by 67%) and decreased solubility (from 96.6% to 43.4%) compared to PPI films, suggesting
that the incorporation of xylose could markedly enhance the mechanical properties and the water
resistance of films. This property improvement was correlated to the increased protein surface
hydrophobicity and sulfhydryl group content with the addition of xylose. No significant differences were
detected among L*, a*, b* colour values of all films (P > 0.05). The scanning electron microscope
images of the PPI–X film showed that the addition of xylose up to 10% produced a more homogeneous
and denser structure. Finally, the improvement mechanism of the PPI–X film was proposed.
Disciplines :
Food science
Author, co-author :
Liu, Li ✱; Université de Liège - ULiège > Form. doct. sc. agro. & ingé. biol. (paysage)
H. Tian G. Xu B. Yang G. Guo Microstructure and mechanical properties of soy protein/agar blend films: Effect of composition and processing methods J. Food Eng. 2011 107 1 21 26
R. Kuktaite T. S. Plivelic Y. Cerenius M. S. Hedenqvist M. Gallstedt S. Marttila R. Ignell Y. Popineau O. Tranquet P. R. Shewry et al., Structure and morphology of wheat gluten films: From polymeric protein aggregates toward superstructure arrangements Biomacromolecules 2011 12 5 1438 1448
N. Reddy L. Chen Y. Yang Thermoplastic films from peanut proteins extracted from peanut meal Ind. Crops Prod. 2013 43 1 159 164
W. J. Lin H. Z. Liu A. M. Shi L. Liu B. Adhikari Q. Wang Preparation and characterisation of films from xylose-glycosylated peanut protein isolate powder Int. J. Food Sci. Technol. 2015 50 7 1538 1544
N. Reddy Q. Jiang Y. Yang Preparation and properties of peanut protein films crosslinked with citric acid Ind. Crops Prod. 2012 39 1 26 30
F. Hammann M. Schmid Determination and quantification of molecular interactions in protein films: A review Materials 2014 7 12 7975 7996
W. J. Lin H. Z. Liu A. M. Shi L. Liu Q. Wang B. Adhikari Effect of glycosylation with xylose on the mechanical properties and water solubility of peanut protein films J. Food Sci. Technol. 2015 52 10 6242 6253
G. Rocha Plácido Moore S. Maria Martelli C. Gandolfo P. José do Amaral Sobral J. Borges Laurindo Influence of the glycerol concentration on some physical properties of feather keratin films Food Hydrocolloids 2006 20 7 975 982
H. Zhang S. Wang X. Zheng L. Jiang X. Lv Y. Shi L. Li Effect of glycosylation on the mechanical properties of edible soy protein packaging film Eur. Food Res. Technol. 2014 238 6 1049 1055
L. M. Pérez G. N. Piccirilli N. J. Delorenzi R. A. Verdini Effect of different combinations of glycerol and/or trehalose on physical and structural properties of whey protein concentrate-based edible films Food Hydrocolloids 2016 56 352 359
C. Li W. Zhu H. Xue Z. Chen Y. Chen X. Wang Physical and structural properties of peanut protein isolate-gum Arabic films prepared by various glycation time Food Hydrocolloids 2015 43 322 328
M. Wihodo C. I. Moraru Physical and chemical methods used to enhance the structure and mechanical properties of protein films: A review J. Food Eng. 2013 114 3 292 302
R. Sothornvit J. M. Krochta Plasticizer effect on mechanical properties of β-lactoglobulin films J. Food Eng. 2001 50 3 149 155
H. Zhang Y. Chi Modified soy protein isolate with improved gelling stability by glycosylation under the conditions of ocean shipping Int. J. Food Sci. Technol. 2011 46 1 14 22
X.-H. He H.-Z. Liu L. Liu G.-L. Zhao Q. Wang Q.-L. Chen Effects of high pressure on the physicochemical and functional properties of peanut protein isolates Food Hydrocolloids 2014 36 123 129
T. Beveridge S. J. Toma S. Nakai Determination of Sh- and Ss-Groups in Some Food Proteins Using Ellman'S Reagent J. Food Sci. 1974 39 1 49 51
C. Li H. Xue Z. Chen Q. Ding X. Wang Comparative studies on the physicochemical properties of peanut protein isolate-polysaccharide conjugates prepared by ultrasonic treatment or classical heating Food Res. Int. 2014 57 1 7
C. H. Tang Y. Jiang Modulation of mechanical and surface hydrophobic properties of food protein films by transglutaminase treatment Food Res. Int. 2007 40 4 504 509
M. Khajehpour J. L. Dashnau J. M. Vanderkooi Infrared spectroscopy used to evaluate glycosylation of proteins Anal. Biochem. 2006 348 1 40 48
J. F. Su X. Y. Yuan Z. Huang X. Y. Wang X. Z. Lu L. D. Zhang S. B. Wang Physicochemical properties of soy protein isolate/carboxymethyl cellulose blend films crosslinked by Maillard reactions: Color, transparency and heat-sealing ability Mater. Sci. Eng., C 2012 32 1 40 46
Ó. L. Ramos I. Reinas S. I. Silva J. C. Fernandes M. A. Cerqueira R. N. Pereira A. A. Vicente M. F. Poças M. E. Pintado F. X. Malcata Effect of whey protein purity and glycerol content upon physical properties of edible films manufactured therefrom Food Hydrocolloids 2013 30 1 110 122
H. Yang S. Yang J. Kong A. Dong S. Yu Obtaining information about protein secondary structures in aqueous solution using Fourier transform IR spectroscopy Nat. Protoc. 2015 10 3 382 396
E. Vass M. Hollósi F. Besson R. Buchet Vibrational spectroscopic detection of beta- and gamma-turns in synthetic and natural peptides and proteins Chem. Rev. 2003 103 5 1917 1954
P. Guerrero K. De La Caba Thermal and mechanical properties of soy protein films processed at different pH by compression J. Food Eng. 2010 100 2 261 269
X. Geng B. Cui Y. Li W. Jin Y. An B. Zhou T. Ye L. He H. Liang L. Wang et al., Preparation and characterization of ovalbumin and carboxymethyl cellulose conjugates via glycosylation Food Hydrocolloids 2014 37 86 92
M. Deleu J. M. Crowet M. N. Nasir L. Lins Complementary biophysical tools to investigate lipid specificity in the interaction between bioactive molecules and the plasma membrane: A review Biochim. Biophys. Acta, Biomembr. 2014 1838 12 3171 3190
Y. Jiang Y. Li Z. Chai X. Leng Study of the physical properties of whey protein isolate and gelatin composite films J. Agric. Food Chem. 2010 58 8 5100 5108
S. Galus J. Kadzińska Whey protein edible films modified with almond and walnut oils Food Hydrocolloids 2016 52 78 86
L. A. Kunte M. A. Hanna C. L. Weller Cast Films from Soy Protein Isolates and Fractions Cereal Chem. 1997 74 2 115 1118
G. L. A. Makishi R. S. Lacerda A. M. Q. B. Bittante H. N. M. Chambi P. A. Costa C. A. Gomide R. A. Carvalho P. J. A. Sobral Films based on castor bean (Ricinus communis L.) proteins crosslinked with glutaraldehyde and glyoxal Ind. Crops Prod. 2013 50 375 382
L. Chin-Chi A. M. Tellez-Garay M. E. Castell-Perez Physical and mechanical properties of peanut protein films Lebensm.-Wiss. Technol. 2004 37 7 731 738
M. Anker M. Stading A.-M. Hermansson Relationship between the Microstructure and the Mechanical and Barrier Properties of Whey Protein Films J. Agric. Food Chem. 2000 48 9 3806 3816
K. Chang J. R. Williamson H. S. Zarkowsky Effect of heat on the circular dichroism of spectrin in hereditary pyropoikilocytosis J. Clin. Invest. 1979 64 1 326 328
F. C. D. Oliveira J. S. D. R. Coimbra E. B. de Oliveira A. D. G. Zuñiga E. E. G. Rojas Food Protein-Polysaccharide Conjugates obtained via the Maillard Reaction: A Review Crit. Rev. Food Sci. Nutr. 2016 56 7 1108 1125
D. Zhu S. Damodaran J. A. Lucey Formation of whey protein isolate (WPI)-dextran conjugates in aqueous solutions J. Agric. Food Chem. 2008 56 16 7113 7118