Article (Scientific journals)
Clinical evaluation of the DermaGenius Nail real-time PCR assay for the detection of dermatophytes and Candida albicans in nails
Hayette, Marie-Pierre; SEIDEL, Laurence; ADJETEY BAHUN, Akolé et al.
2019In Medical Mycology
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Keywords :
real-time PCR; DermaGenius; dermatophytes; candida albicans; nails
Abstract :
[en] Onychomycosis represents one of the most frequent mycoses in the world. Causative agents are mainly dermatophytes, but yeasts and nondermatophyte moulds can also be involved. Conventional diagnostic methods include direct microscopy (or histology) and culturing. However,molecular methods are becoming increasingly popular in this field. The DermaGeniusR (DG) Nail multiplex assay (PathoNostics, The Netherlands) is a new commercial real-time polymerase chain reaction (PCR) kit, which can detect Trichophyton rubrum, Trichophyton interdigitale and Candida albicans directly in nails. The present study is a retrospective evaluation of the kit applied to 138 finger and toenail clippings in comparison to histology and culture methods. The sensitivity and specificity of the PCR assay are 80% (76/95) and 74.4% (32/43), respectively,when histology and culture are used as reference to define onychomycosis. DG performance is not different from histology combined with culture (P = .11) but the best diagnostic efficacy (88.4%, 122/138) is obtained by the combination of histology and DG. In conclusion, this study emphasizes the clinical usefulness of the DG in diagnostics. The high specificity of this test guarantees a better identification compared to culture that can lead to dermatophyte misidentifications. It is a reliable PCR assay that shortens the time to diagnosis and can unmask the presence of nongrowing fungal pathogens in nails.
Disciplines :
Laboratory medicine & medical technology
Author, co-author :
Hayette, Marie-Pierre ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Bactériologie, mycologie, parasitologie, virologie
SEIDEL, Laurence  ;  Centre Hospitalier Universitaire de Liège - CHU > Service des informations médico économiques (SIME)
ADJETEY BAHUN, Akolé ;  Centre Hospitalier Universitaire de Liège - CHU > Service de microbiologie clinique
DARFOUF, Rajae ;  Centre Hospitalier Universitaire de Liège - CHU > Service de microbiologie clinique
WERY, Marie ;  Centre Hospitalier Universitaire de Liège - CHU > Service de microbiologie clinique
BOREUX, Raphaël ;  Centre Hospitalier Universitaire de Liège - CHU > Service de microbiologie clinique
SACHELI, Rosalie  ;  Centre Hospitalier Universitaire de Liège - CHU > Service de microbiologie clinique
Melin, Pierrette  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Bactériologie, mycologie, parasitologie, virologie
Arrese Estrada, Jorge ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Language :
English
Title :
Clinical evaluation of the DermaGenius Nail real-time PCR assay for the detection of dermatophytes and Candida albicans in nails
Alternative titles :
[fr] Evaluation clinique du kit DermaGenius Nail real-time PCR pour la détection de dermatophytes et de Candida albicans dans les ongles
Publication date :
April 2019
Journal title :
Medical Mycology
ISSN :
1369-3786
eISSN :
1460-2709
Publisher :
Taylor & Francis, United Kingdom
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 13 May 2018

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