Platelet-rich plasma; PRP; rat; Achilles tendon; tendon healing; animal model
Abstract :
[en] This article describes the experimental procedures used to observe if PRP can positively affect tendon healing. There are 4 main steps to
follow: induce a lesion in the Achilles tendon; prepare PRP and inject it (or the saline solution); remove the tendon; and perform biomechanical,
molecular, and histological evaluations. At each step, all the procedures and methods are described in detail, so they can be reproduced easily.
Achilles tendons have been surgically sectioned (removal of a 5-mm long section). Afterwards, PRP or saline solution was injected to study
whether PRP has a positive effect on the healing of the tendon. Three groups of 40 animals (a total of 120 rats were used in this study) were
subdivided into 2 subgroups: PRP injection group and a saline injection control group. Rats were sacrificed at increasing time points (Group
A: 5 days; Group B: 15 days; Group C: 30 days) and tendons were removed. 90 tendons underwent biomechanical testing before performing
transcriptomic analysis and the 30 remaining tendons were submitted to histological analysis.
Disciplines :
Orthopedics, rehabilitation & sports medicine Laboratory medicine & medical technology
Author, co-author :
Greimers, Laura
Drion, Pierre ; Université de Liège > Département des sciences biomédicales et précliniques > GIGA-R:Méth. expér.des anim. de labo et éth. en expér. anim.
Colige, Alain ; Université de Liège > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs
Libertiaux, Vincent ; Université de Liège > Clinique des grands animaux (chirurgie)
Denoël, Vincent ; Université de Liège > Département ArGEnCo > Analyse sous actions aléatoires en génie civil
LECUT, Christelle ; Centre Hospitalier Universitaire de Liège - CHU > Service d'hématologie biologique et immuno-hématologie
Gothot, André ; Université de Liège > Département des sciences biomédicales et précliniques > Biologie de la coagulation et de l'hémostase
Kaux, Jean-François ; Université de Liège > Département des sciences de la motricité > Médecine physique, réadaptation et traumatologie du sport
Language :
English
Title :
Effects of Allogeneic Platelet-Rich Plasma (PRP) on the Healing Process of Sectioned Achilles Tendons of Rats: A Methodological Description
Publication date :
March 2018
Journal title :
Journal of Visualized Experiments
eISSN :
1940-087X
Publisher :
MYJoVE Corporation, Boston, United States - Massachusetts
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