A new method for the determination of the relative affinity of a ligand against various DNA sequences by electrospray ionization mass spectrometry. Application to a polyamide minor groove binder
Buchmann, W.; Boutorine, A.; Halby, L.et al.
2009 • In Journal of Mass Spectrometry, 44, p. 1171-1181
[en] A new method for the determination of the relative affinity of a ligand against various dsDNA sequences is presented by using electrospray ionization time-of-flight mass spectrometry (ESI-QTOF) mass spectrometry. Thismethod does not require knowing the ligand concentration accurately. It allows determination of the relative affinity of a ligand against various dsDNA sequences for 1 : 1 complex stoichiometries in a quick manner without labeling.
Boutorine, A.; Museum Natl Hist Nat, RDDM, USM 0503, F-75231 Paris 05, France > INSERM, U565, Paris, France > CNRS, UMR 5153, Paris, France
Halby, L.; Museum Natl Hist Nat, RDDM, USM 0503, F-75231 Paris 05, France > INSERM, U565, Paris, France > CNRS, UMR 5153, Paris, France
Tortajada, J.; Univ Evry Val Essonne > CNRS UMR 8587, CEA, F-91025 Evry
De Pauw, Edwin ; Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)
Language :
English
Title :
A new method for the determination of the relative affinity of a ligand against various DNA sequences by electrospray ionization mass spectrometry. Application to a polyamide minor groove binder
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