Reference : Analysis of EBV Transcription Using High-Throughput RNA Sequencing.
Scientific journals : Article
Human health sciences : Multidisciplinary, general & others
http://hdl.handle.net/2268/218507
Analysis of EBV Transcription Using High-Throughput RNA Sequencing.
English
O'Grady, Tina mailto [Université de Liège - ULiège > Département des sciences de la vie > Génétique et biologie moléculaires animales >]
Baddoo, Melody [> >]
Flemington, Erik K. [> >]
2017
Methods in Molecular Biology
1532
105-121
Yes (verified by ORBi)
International
1064-3745
United States
[en] Differential expression ; EBSeq ; Epstein-Barr virus ; IGV ; Integrative genomics viewer ; RNA-Seq ; RSEM ; STAR ; Transcriptomics
[en] High-throughput sequencing of RNA is used to analyze the transcriptomes of viruses and cells, providing information about transcript structure and abundance. A wide array of programs and pipelines has been created to manage and interpret the abundance of data generated from high-throughput RNA sequencing experiments. This protocol details the use of free and open-source programs to align RNA-Seq reads to a reference genome, visualize read coverage and splice junctions, estimate transcript abundance, and evaluate differential expression of transcripts in different conditions. Particular concerns related to EBV and viral transcriptomics are addressed and access to EBV reference files is provided.
http://hdl.handle.net/2268/218507
10.1007/978-1-4939-6655-4_7

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