Reference : Analysis of EBV Transcription Using High-Throughput RNA Sequencing.
Scientific journals : Article
Human health sciences : Multidisciplinary, general & others
Analysis of EBV Transcription Using High-Throughput RNA Sequencing.
O'Grady, Tina mailto [Université de Liège - ULiège > Département des sciences de la vie > Génétique et biologie moléculaires animales >]
Baddoo, Melody [> >]
Flemington, Erik K. [> >]
Methods in Molecular Biology
Yes (verified by ORBi)
United States
[en] Differential expression ; EBSeq ; Epstein-Barr virus ; IGV ; Integrative genomics viewer ; RNA-Seq ; RSEM ; STAR ; Transcriptomics
[en] High-throughput sequencing of RNA is used to analyze the transcriptomes of viruses and cells, providing information about transcript structure and abundance. A wide array of programs and pipelines has been created to manage and interpret the abundance of data generated from high-throughput RNA sequencing experiments. This protocol details the use of free and open-source programs to align RNA-Seq reads to a reference genome, visualize read coverage and splice junctions, estimate transcript abundance, and evaluate differential expression of transcripts in different conditions. Particular concerns related to EBV and viral transcriptomics are addressed and access to EBV reference files is provided.

File(s) associated to this reference

Fulltext file(s):

Open access
OGrady_et_al_MMB_2017.pdfAuthor preprint198.96 kBView/Open

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.