[en] Pea starch, hulls and cotyledon inner fibres, isolated from pea seeds, were incorporated in N-free diets, on a NDF-content basis, in order to study their effect on the ileal endogenous amino acid (AA) excretion in the growing pig. Maize starch and wood cellulose were selected as references. The inner fibrecontaining diet was also supplemented with enzymatically hydrolysed casein (EHC) in order to test the peptide alimentation method on a diet containing a fibre source with a high water-holding capacity (WHC = 10.9 g water g-' DM). The fibres were also analysed by different methods (crude fibre, NDF, ADF, enzymatic-gravimetric method, Englyst method). The fibre content of the inner fibre fraction varied widely from one method to another and ranged from 109 g crude fibre to 480 g AOAC fibre kg-' DM. Compared to the 'maize starch + wood cellulose' diet (11.0 g AA excreted kg-' DM intake), pea starch had no effect on ileal AA losses (9.6 g), whereas pea hulls slightly increased them (14.2 g). The AA excretion dramatically increased with the incorporation of inner fibres (28.0 g), because of their very high WHC. The addition of EHC increased the output (48.0 g) further, at a level that can hardly be explained only by an increase of the endogenous secretions. The results suggest that the NDF content of grain legume products is not indicative of their effect on the ileal endogenous AA losses in pigs and that the physiological effects of fibres along the gastrointestinal tract are due to both their chemical and physical properties.
Research Center/Unit :
TNO-ILOB, Wageningen, Netherland
Disciplines :
Agriculture & agronomy Animal production & animal husbandry
Author, co-author :
Leterme, Pascal; Faculté Universitaire des Sciences Agronomiques de Gembloux - FUSAGx > Unité de Zootechnie
Thewis, André ; Université de Liège - ULiège > Gembloux Agro-Bio Tech
Van leeuwen, Piet; ILOB-TNO, Wageningen
Monmart, Thierry; Faculté Universitaire des Sciences Agronomiques de Gembloux - FUSAGx > Unité de Zootechnie
Huisman, Joop; ILOB-TNO, Wageningen
Language :
English
Title :
Chemical Composition Of Pea Fibre Isolates And Their Effect On The Endogenous Amino Acid Flow At The Ileum Of The Pig
Publication date :
1996
Journal title :
Journal of the Science of Food and Agriculture
ISSN :
0022-5142
eISSN :
1097-0010
Publisher :
John Wiley & Sons, Inc, Chichester, United Kingdom
Auffret A, Guillon F, Barry J, Ralet M, Thibault J 1994 Effect of grinding and experimental conditions on the measurement of hydration properties of dietary fibres. Lebensm Wiss Technol 12 247-251.
Bach Knudsen K, Borg Jensen B, Andersen J, Hansen I 1991 Gastrointestinal implications in pigs of wheat and oat fractions. 2. Microbial activity in the gastrointestinal tract. Br J Nutr 65 233-248.
Butts C, Moughan P, Smith W 1991 Endogenous amino acid flow at the terminal ileum of the rat determined under conditions of peptide alimentation. J Sci Food Agric 55 175-187.
Butts C, Moughan P, Smith W 1992 Protein nitrogen, peptide nitrogen and free amino acid nitrogen in endogenous digesta nitrogen at the terminal ileum of the rat. J Sci Food Agric 59 291-298.
Butts C, Moughan P, Smith W, Carr D 1993 Endogenous lysine and other amino acid flows at the terminal ileum of the growing pig (20 kg bodyweight): the effect of protein-free, synthetic amino acid, peptide and protein alimentation. J Sci Food Agric 61 31-40.
Cherbut C, Bruley des Varannes S, Schnee M, Rival M, Bamiche J, Delort-Laval J 1994 Involvment of small intestinal motility in blood glucose response to dietary fibre in man. Br J Nutr 71 675-685.
Costa N, Low G, Walker A, Owen R, Englyst H 1994 Effect of baked on steroid metabolism and non-starch polysaccharide output of hypercholesteroleamic pigs with or without an ileo-rectal anastomosis. Br J Nutr 71 871-886.
Decuypere J, Spriet S, van Gils L 1994 Influence of the water-holding capacity (WHC) of the feed on the precaecal and fecal apparent digestibility in pigs. In: Proc VIth Int Symp on Digestive Physiology in Pigs, eds Souffrant W & Hagemeister H. EAAP Publ no 80, pp 125-128.
Dierick N, Vervaeke I, Decuypere J, Henderickx H 1983 Influence de la nature et du niveau des fibres brutes sur la digestibilité iléale et fécale apparente de la matrière sèche, des protéines et des acides aminés et sur la rétentions azotée chez les porcs. Rev Agric (Brussels) 36 1691-1711.
Englyst H, Cummings J 1988 Improved method for measurement of dietary fibre as non-starch polysaccharides in plant foods. J AOAC 71 808-814.
Englyst H, Quigley M, Hudson G, Cummings J 1992 Determination of dietary fibre as non-starch polysaccharides by gas-liquid chromatography. Analyst 117 1707-1714.
François E, Thill N, Théwis A 1978 Méthode rapide de dosage de l'oxyde de chrome dans les aliments, les fèces et les contenus digestifs par titrage après oxydation nitroperchlorique. Ann Zootech 27 355-361.
Furuya S, Kaji Y 1992 The effects of feed intake and purified cellulose on the endogenous ileal amino acid flow in growing pigs. Br J Nutr 68 463-472.
Geil P, Anderson J 1994 Nutrition and health implications of dry beans: a review. J Am College Nutr 13 549-558.
Giger S, Thivend P, Sauvant D, Dorléans M, Hournaix P 1987 Etude de l'influence préalable de différentes enzymes amylolytiques sur la teneur en résidu NDF d'aliments du bétail. Ann Zootech 36 39-48.
Goodlad J, Mathers J 1990 Large bowel fermentation in rats given diets containing raw peas. Br J Nutr 64 569-587.
Goodlad J, Mathers J 1992 Digestion of complex carbohydrates and large bowel fermentation in rats fed on raw and cooked peas. Br J Nutr 67 475-488.
Goodlad R, Ratcliffe B, Fordham J, Wright N 1989 Does dietary fibre stimulate intestinal epithelial cell proliferation in germ-free rats. Gut 30 820-825.
Hansen I, Bach Knudsen K, Eggum B 1992 Gastrointestinal implications in the rat of wheat bran, oat bran and pea fibre. Br J Nutr 68 451-462.
Huisman J, Heinz T, Poel A van der, Leeuwen P van, Souffrant W, Verstegen M 1992 True ileal digestibility and amounts of endogenous protein measured with the 15N-dilution technique in piglets fed on peas (Pisum sativum) and common beans (Phaseolus vulgaris). Br J Nutr 68 101-110.
Jin L, Reynolds L, Redmer D, Caton J, Crenshaw J 1994 Effects of dietary fibre on intestinal growth, cell proliferation and morphology in growing pigs. J Anim Sci 72 2270-2278.
Krawielitzki K, Zebrowska T, Schadereit R, Kowalczyk J, Wünsche J, Hermann U 1990 Determining of nitrogen absorption and nitrogen secretion in different sections of the pig's intestine by digesta exchange between 15N-labelled and unlabelled animals. Arch Tierernährung 40 25-37.
Leeuwen P van, Kleef D van, Kempen G van, Huisman J, Verstegen M 1991 The post-valve T-caecum cannulation technique: an alternative method for chyme collection in pigs. J Anim Physiol Anim Nutr 65 183-193.
Leterme P, Monmart M 1990 Importance du tryptophane en alimentation et son dosage dans les aliments. Bull Rech Agron (Gembloux) 25 29-344.
Leterme P, Pirard L, Théwis A 1992 A note on the effect of wood cellulose level in protein-free diets on the recovery and amino acid composition of endogenous protein collected from the ileum in pigs. Anim Prod 54 163-165.
Lien K, Sauer W, McBurney M 1994 The effect of pea fibre addition on ileal and fecal amino acid digestibilities, carbohydrate recoveries and ileal mucus output. In: Proc VIth Int Symp on Digestive Physiology in Pigs, eds Souffrant W & Hagemeister H. EAAP Publ, pp 75-78.
MacKenzie S, Tenaschuk D 1979a Quantitative formation of N (O, S)-heptafluorobutyryl isobutyl amino acids for gas Chromatographic analyses. I. Esterification. J Chromatogr 171 195-208.
MacKenzie S, Tenaschuk D 1979b Quantitative formation of N (O, S)-heptafluorobutyryl isobutyl amino acids for gas Chromatographic analyses. II. Acylation. J Chromatogr 173 53-63.
Mariscal-Landin G, Sève B, Colleaux Y, Lebreton Y 1995 Endogenous amino nitrogen collected from pigs with end-to-end ileorectal anastomosis is affected by the method of estimation and altered by dietary fibre. J Nutr 125 136-146.
Mathers J 1991 Digestion of non-starch polysaccharides by non-ruminant omnivores. Proc Nutr Soc 50 161-172.
Mathers J, Kennard J, James O 1993 Gastrointestinal responses to oats consumption in young adult and elderly rats: digestion, large bowel fermentation and crypt cell proliferation rates. Br J Nutr 70 567-584.
Mongeau R, Brassard R 1994 Comparison and assessment of the difference in total dietary fibre in cooked dried legumes as determined by five methods. J AOAC 77 1197-1202.
Moughan P, Darragh A, Smith W, Butts C 1990 Perchloric and trichloroacetic acids as precipitants of protein in endogenous ileal digesta from the rat. J Sci Food Agric 52 13-21.
Murray A, Fuller M, Pirie A 1977 The effect of fibre in the form of various polysaccharides on the apparent digestibility of protein in the pig. Anim Prod 24 139-144.
Periago M, Ros G, Englyst H, Rincon F 1994 Variation in dietary fibre content of peas as function of variety, size and analytical method. Revista Española de Ciencia y Tecnología de Alimentas 34 565-575.
Prosky L, Asp N, Schweizer T, De Vries J, Furda I 1988 Determination of insoluble, soluble and total dietary fibre in foods and food products. J AOAC 71 1017-1023.
Prosky L, Asp N, Schweizer T, De Vries J, Furda I, Lee S 1994 Determination of soluble dietary fibre in foods and food products: collaborative study. J AOAC 77 690-694.
Ralet M, Della Valle G, Thibault J 1993 Raw and extruded fibre from pea hulls. Part I: Composition and physicochemical properties. Carbohydr Polym 20 17-23.
Sakata T 1987 Stimulatory effect of short-chain fatty acids on epithelial cell proliferation in the rat intestine: possible explanation for the trophic effects of fermentable fibre, gut microbes and luminal trophic factors. Br J Nutr 58 95-103.
Sandström B, Hansen L, Sørensen A 1994 Pea fibre lowers fasting and postprandial blood triglyceride concentrations in humans. J Nutr 124 2386-2396.
Schneeman B, Richter D, Jacobs L 1982 Response to dietary wheat bran in the exocrine pancreas and intestine of rats. J Nutr 112 283-290.
Schulze H, Leeuwen P van, Verstegen M, Huisman J, Souffrant W, Ahrens F 1994 Effects of level of dietary neutral detergent fibre on ileal apparent digestibility and ileal nitrogen losses in pigs. J Anim Sci 72 2362-2368.
Schulze H, Leeuwen P van, Verstegen M, van den Berg J 1995 Dietary level of neutral detergent fibre and ileal endogenous nitrogen flow in pigs. J Anim Sci 73 441-448.
Scott R 1979 Colorimetric determination of hexuronic acids in plant materials. Anal Chem 31 936-941.
Slump P, Schreuder H 1969 Determination of tryptophan in foods. Anal Biochem 27 182-185.
Soest J van, Wine R 1967 Use of detergents in the analysis of fibrous feeds. Determination of plant cell-wall constituents. J AO AC 50 50-55.
Souffrant W, Rérat A, Laplace J P, Darcy-Vrillon B, Köhler R, Corring T, Gebhardt G 1993 Exogenous and endogenous contributions to nitrogen fluxes in the digestive tract of pigs fed a casein diet. III. Recycling of endogenous nitrogen. Reprod Nutr Dévelop 33 373-382.
Weerden E van, Slump P, Huisman J 1980 Amino acid digestion in different parts of the intestinal tract of pigs. In: Proc 3rd EAAP-Symposium on Protein and Metabolism and Nutrition, eds Oslage H & Rohr K. EAAP Publ, pp 207-214.
Wolters G, Verbeek C, van Westerop J, Hermus R, Voragen A 1992 Comparison of different methods for determination of dietary fibre. J AO AC 75 626-634.
Zhao X, Jørgensen H, Eggum B 1995 The influence of dietary fibre on body composition, visceral organ weight, digestibility and energy balance in rats housed in different thermal environments. Br J Nutr 73 687-699.
