The 13C- vs the 15N-leucine infusion technique for the determination of the ileal endogenous N losses in pigs.

Leterme, Pascal; Thewis, André; Seve, Bernard

Reference : The 13C- vs the 15N-leucine infusion technique for the determination of the ileal end...


	Document type :	Scientific congresses and symposiums : Paper published in a book
	Discipline(s) :	Life sciences : Anatomy (cytology, histology, embryology...) & physiology Life sciences : Animal production & animal husbandry
	To cite this reference:	http://hdl.handle.net/2268/21724

Title :	The 13C- vs the 15N-leucine infusion technique for the determination of the ileal endogenous N losses in pigs.
Language :	English
Author, co-author :	Leterme, Pascal [Faculté Universitaire des Sciences Agronomiques de Gembloux - FUSAGx > > Unité de Zootechnie > >]
	Thewis, André [Université de Liège - ULiège > > Gembloux Agro-Bio Tech >]
	Seve, Bernard [Institut Scientifique de Recherche Agronomique - INRA > > Station de Recherches Porcines > >]
Publication date :	1997
Main document title :	Digestive Physiology of Pigs
Alternative title :	[fr] Physiologie Digestive du Porc
Author, co-author :	Lindberg, J. E.
	Ogle, Brian
Publisher :	CABI Publishing
Pages :	596-599
Peer reviewed :	Yes
Audience :	International
City :	New York
Country :	NY
Event name :	Digestive physiology in pigs. Proceedings of the 7th InternationalSymposium Saint Malo, France 26-28 May 1997.
Event organizer :	Department of Animal Nutrition and Management Swedish University of Agricultural Science Uppsala, Sweden
Event place (city) :	Uppsala
Event country :	Sweden
Keywords :	[en] Pig ; Endogenous nitrogen ; Isotope dilution ; Methodology
	[fr] Porc ; Azote endogène ; Dilution isotopique ; Méthodologie
Abstract :	[en] Some methodological aspects of the isotope dilution technique using 15N-leucine as a marker of the endogenous N secretions (choice of the precursor pool, time of blood collection, estimation of the isotopique equilibrium) were studied in pigs. Their effect on the estimation of the ileal endogenous N flow was limited. When 15N-leucine was replaced by 13C-leucine, the ileal endogenous N flow obtained with 13C was much lower than that obtained with 15N (1.4 vs 5.7g N/kg DM intake, respectively). The validity of the 13C-leucine infusion technique is questioned.
Funders :	Ministère Belge de l'Agriculture - Département Recherches et Développement
Target :	Researchers
Permalink :	http://hdl.handle.net/2268/21724
Commentary :	2-7380-0749-X

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