Reference : The 13C- vs the 15N-leucine infusion technique for the determination of the ileal end...
Scientific congresses and symposiums : Paper published in a book
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
Life sciences : Animal production & animal husbandry
The 13C- vs the 15N-leucine infusion technique for the determination of the ileal endogenous N losses in pigs.
Leterme, Pascal [Faculté Universitaire des Sciences Agronomiques de Gembloux - FUSAGx > > Unité de Zootechnie > >]
Thewis, André mailto [Université de Liège - ULiège > > Gembloux Agro-Bio Tech >]
Seve, Bernard mailto [Institut Scientifique de Recherche Agronomique - INRA > > Station de Recherches Porcines > >]
Digestive Physiology of Pigs
[fr] Physiologie Digestive du Porc
Lindberg, J. E.
Ogle, Brian
CABI Publishing
New York
Digestive physiology in pigs. Proceedings of the 7th InternationalSymposium Saint Malo, France 26-28 May 1997.
Department of Animal Nutrition and Management Swedish University of Agricultural Science Uppsala, Sweden
[en] Pig ; Endogenous nitrogen ; Isotope dilution ; Methodology
[fr] Porc ; Azote endogène ; Dilution isotopique ; Méthodologie
[en] Some methodological aspects of the isotope dilution technique using 15N-leucine as a marker of the endogenous N secretions (choice of the precursor pool, time of blood collection, estimation of the isotopique equilibrium) were studied in pigs. Their effect on the estimation of the ileal endogenous N flow was limited. When 15N-leucine was replaced by 13C-leucine, the ileal endogenous N flow obtained with 13C was much lower than that obtained with 15N (1.4 vs 5.7g N/kg DM intake, respectively). The validity of the 13C-leucine infusion technique is questioned.
Ministère Belge de l'Agriculture - Département Recherches et Développement

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