Article (Scientific journals)
Comprehensive volatile metabolic fingerprinting of bacterial and fungal pathogen groups
Rees, Christiaan; Burklund, Alison; Stefanuto, Pierre-Hugues et al.
2017In Journal of Breath Research
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Keywords :
Bacterial infections; Fungal infections; Metabolic fingerprinting
Abstract :
[en] The identification of pathogen-specific volatile metabolic "fingerprints" could lead to the rapid identification of disease-causing organisms either directly from <i>ex vivo</i> patient bio-specimens or from <i>in vitro</i> cultures. In the present study, we have evaluated the volatile metabolites produced by 100 clinical isolates belonging to ten distinct pathogen groups that, in aggregate, account for 90 % of bloodstream infections, 90 % of urinary tract infections, and 80 % of infections encountered in the intensive care unit setting. Headspace volatile metabolites produced in vitro were concentrated using headspace solid-phase microextraction and analyzed via two-dimensional gas chromatography time-of-flight mass spectrometry (HS-SPME-GC×GC-TOFMS). A total of 811 volatile metabolites were detected across all samples, of which 203 were: 1) detected in 9 or 10 (of 10) isolates belonging to one or more pathogen groups, and 2) significantly more abundant in cultures relative to sterile media. Network analysis revealed a distinct metabolic fingerprint associated with each pathogen group, and analysis via Random Forest (RF) using leave-one-out cross-validation (LOOCV) resulted in a 95 % accuracy for the differentiation between groups. The present findings support the results of prior studies that have reported on the differential production of volatile metabolites across pathogenic bacteria and fungi, and provide additional insight through the inclusion of pathogen groups that have seldom been studied previously, including <i>Acinetobacter</i> spp., coagulase-negative <i>Staphylococcus</i>, and <i>Proteus mirabilis</i>, as well as the utilization of HS-SPME-GC×GC-TOFMS for improved sensitivity and resolution relative to traditional gas chromatography-based techniques.
Disciplines :
Chemistry
Author, co-author :
Rees, Christiaan
Burklund, Alison
Stefanuto, Pierre-Hugues  ;  Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique, organique et biologique
Schwartzman, Joseph
Hill, Jane
Language :
English
Title :
Comprehensive volatile metabolic fingerprinting of bacterial and fungal pathogen groups
Publication date :
September 2017
Journal title :
Journal of Breath Research
ISSN :
1752-7155
eISSN :
1752-7163
Publisher :
Institute of Physics (IOP), Bristol, United Kingdom
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 01 December 2017

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