Comparison of nanofluidic and ultra-high performance liquid chromatography-tandem mass spectrometry for high sensitive pharmacokinetic studies of estrogens starting from whole blood microsampling

Nys, Gwenaël; COBRAIVILLE, Gaël; Kok, Miranda; Wéra, Odile; Servais, Anne-Catherine; Fillet, Marianne

doi:10.1016/j.chroma.2017.10.006

Article (Scientific journals)

Comparison of nanofluidic and ultra-high performance liquid chromatography-tandem mass spectrometry for high sensitive pharmacokinetic studies of estrogens starting from whole blood microsampling

Nys, Gwenaël; COBRAIVILLE, Gaël; Kok, Miranda et al.

2017 • In Journal of Chromatography. A, 1524, p. 160-168

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/216201

DOI
10.1016/j.chroma.2017.10.006

PubMed
29017723

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Keywords :

Estrogens; Mass spectrometry; Nanofluidic LC-chip-MS/MS; Pharmacokinetic study; UHPLC-MS/MS; Whole blood microsampling; Blood; High performance liquid chromatography; Liquid chromatography; Pharmacokinetics; Spectrometry; Limit of quantitations; Optimal conditions; Pharmacokinetic studies; Solvent consumption; U-HPLC-MS; Ultra-high performance liquid chromatographies; Whole blood; Nanofluidics

Abstract :

[en] Pharmacokinetic (PK) studies on small animals are challenging as only small volumes of samples are available, in which the analyte is present at low concentration in a complex matrix. In this context, the use of miniaturized analytical techniques may provide undeniable advantages in terms of sensitivity, sample and solvent consumption compared to the reference UHPLC-MS/MS methods In this study, we present the development of a nanofluidic-LC-MS/MS method to analyze two model analytes of therapeutic interest, namely estradiol (E2) and estetrol (E4) after microsampling with volumetric absorptive microsampling (VAMS) devices, an innovative sampling technique to collect small volumes of whole blood. The nanofluidic LC-MS/MS method was developed using an experimental design to find the optimal conditions to analyze both E2 and E4 with the highest sensitivity. Subsequently, the optimized method was validated according to ICH guidelines and compared to a previously developed UHPLC-MS/MS method. A limit of quantitation of 50. pg/ml was reached with the LC-chip method, which is 50 times better than UHPLC-MS/MS. Both methods were then critically evaluated from the analytical and operational points of view. Finally, the quantitation of estrogens after whole blood microsampling was compared with the results obtained with the corresponding plasma samples. © 2017 Elsevier B.V.

Disciplines :

Pharmacy, pharmacology & toxicology

Author, co-author :

Nys, Gwenaël ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments

COBRAIVILLE, Gaël ; Centre Hospitalier Universitaire de Liège - CHU > Service de rhumatologie

Kok, Miranda ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments

Wéra, Odile ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > GIGA-R : GIGA - Cardiovascular Sciences

Servais, Anne-Catherine ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments

Fillet, Marianne ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments

Title :

Comparison of nanofluidic and ultra-high performance liquid chromatography-tandem mass spectrometry for high sensitive pharmacokinetic studies of estrogens starting from whole blood microsampling

Publication date :

November 2017

Journal title :

Journal of Chromatography. A

ISSN :

0021-9673

eISSN :

1873-3778

Publisher :

Elsevier B.V.

Volume :

1524

Pages :

160-168

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 19 November 2017

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