[en] The aim of this paper was to optimize extraction conditions of C. cardunculus rennet by response surface methodology, in order to maximize its milk-clotting activity (MCA). The second objective was to examine the effect of different amounts of the optimized extract and the type of milk, on technological characteristics of the obtained gels. Results of the central composite design showed that the optimum extraction conditions, corresponding to maximum MCA (9.550 CAU/mL), were selected as follows: Grinding time 30 s, pH 3, extraction time 50 min and solid to liquid ratio 15 g/100 mL. According to technological properties, it can be concluded that C. cardunculus extract exhibited an excellent efficiency on raw milk, in term of dynamic moduli and gel firmness, as compared to chymosin. A good gelation time and WHC were also obtained. Consequently, the optimized C. cardunculus rennet has the potential to be employed as an efficient milk-clotting agent.
Disciplines :
Food science
Author, co-author :
Ben Amira, Amal
Mokni, Abir
Yaich, Hela
Chaabouni, Moncef
Besbes, Souhail
Blecker, Christophe ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies
Attia, Hamadi
Language :
English
Title :
Technological properties of milk gels produced by chymosin and wild cardoon rennet optimized by response surface methodology
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Bibliography
Barros, R.M., Ferreira, C.A., Silva, S.V., Malcata, F.X., Quantitative studies on the enzymatic hydrolysis of milk proteins brought about by cardosins precipitated by ammonium sulfate. Enzyme and Microbial Technology 29:8 (2001), 541–547.
Beka, R.G., Krier, F., Botquin, M., Guiama, V.D., Donn, P., Libouga, D.G., Guillochon, D., Characterisation of a milk-clotting extract from Balanites aegyptiaca fruit pulp. International Dairy Journal 34:1 (2014), 25–31.
Ben Amira, A., Bauwens, J., De Pauw, E., Besbes, S., Attia, H., Francis, F., Blecker, C., Identification of proteins from wild cardoon flowers (Cynara cardunculus L.) by a proteomic approach. Journal of Chemical Biology, 2016, 1–9.
Ben Amira, A., Makhlouf, I., Flaviu Petrut, R., Francis, F., Bauwens, J., Attia, H., Blecker, C., Effect of extraction pH on techno-functional properties of crude extracts from wild cardoon (Cynara cardunculus L.) flowers. Food Chemistry 225 (2017), 258–266.
Berridge, N., The purification and crystallization of rennin. Biochemical Journal, 39(2), 1945, 179.
Bohlin, L., Hegg, P.-O., Ljusberg-Wahren, H., Viscoelastic properties of coagulating milk. Journal of Dairy Science 67:4 (1984), 729–734.
Brutti, C.B., Pardo, M.F., Caffini, N.O., Natalucci, C.L., Onopordum acanthium L. (Asteraceae) flowers as coagulating agent for cheesemaking. LWT – FoodScience and Technology 45:2 (2012), 172–179.
Carlson, R., Carlson, J.E., Design and optimization in organic synthesis, Vol. 24, 2005, Elsevier.
Cavalli, S.V., Lufrano, D., Colombo, M.L., Priolo, N., Properties and applications of phytepsins from thistle flowers. Phytochemistry 92 (2013), 16–32.
Davis, J.G., Cheese. Vol. III. Manufacturing methods, 1976, Churchill Livingstone.
Dill, C.W., Roberts, W., Monroe, R., Interrelationships of heat treatment, solids-not-fat, and calcium chloride to the yield of cottage cheese. Journal of Dairy Science, 41, 1958, 714 Amer Dairy Science Assoc, 1111 N Dunlap Ave, Savoy, IL 61874.
Jacob, M., Jaros, D., Rohm, H., Recent advances in milk clotting enzymes. International Journal of Dairy Technology 64:1 (2011), 14–33.
Karleskind, D., Laye, I., Mei, F.I., Morr, C., Gelation properties of lipid-reduced, and calcium-reduced whey protein concentrates. Journal of Food Science 60:4 (1995), 731–737.
Klandar, A., Lagaude, A., Chevalier-Lucia, D., Assessment of the rennet coagulation of skim milk: A comparison of methods. International Dairy Journal 17:10 (2007), 1151–1160.
Mazorra-Manzano, Angel M., Moreno-Hernández, J.M., Ramírez-Suarez, J.C., de Jesús Torres-Llanez, M., González-Córdova, A.F., Vallejo-Córdoba, B., Sour orange Citrus aurantium L. flowers: a new vegetable source of milk-clotting proteases. LWT – Food Science and Technology 54:2 (2013), 325–330.
Mazorra-Manzano, M.A., Perea-Gutiérrez, T.C., Lugo-Sánchez, M.E., Ramirez-Suarez, J.C., Torres-Llanez, M.J., González-Córdova, A.F., Vallejo-Cordoba, B., Comparison of the milk-clotting properties of three plant extracts. Food Chemistry 141:3 (2013), 1902–1907.
Ordiales, E., Martín, A., Benito, M.J., Hernández, A., Ruiz-Moyano, S., Córdoba, M.D.G., Technological characterisation by free zone capillary electrophoresis (FCZE) of the vegetable rennet (Cynara cardunculus) used in “Torta del Casar” cheese-making. Food Chemistry 133:1 (2012), 227–235.
Parnell-Clunies, E.M., Kakuda, Y., Deman, J., Influence of heat treatment of milk on the flow properties of yoghurt. Journal of Food Science 51:6 (1986), 1459–1462.
Pearson, A., Saar, H., The manufacture of cottage cheese from dry skimmed milk. Canadian Dairy and Ice Cream Journal 39:1 (1960), 53–54.
Remeuf, F., Lenoir, J., Duby, C., Letilly, M.-T., Normand, A., Etude des relations entre les caractéristiques physico-chimiques des laits de chèvre et leur aptitude à la coagulation par la présure. Le Lait 69:6 (1989), 499–518.
Saint-Denis, T., Goupy, J., Optimization of a nitrogen analyser based on the Dumas method. Analytica Chimica Acta 515:1 (2004), 191–198.
Sousa, M., Malcata, F., Effects of processing conditions on the caseinolytic activity of crude extracts of Cynara cardunculus L./Efectos de las condiciones de extracción sobre la actividad caseinolítica de los extractos de Cynara cardunculus L. Food Science and Technology International 2:4 (1996), 255–263.
Sousa, M.J., Malcata, F.X., Advances in the role of a plant coagulant (Cynara cardunculus) in vitro and during ripening of cheeses from several milk species. Le Lait 82:2 (2002), 151–170.
Tavaria, F.K., Sousa, M.J., Malcata, F.X., Storage and lyophilization effects of extracts of Cynara cardunculus on the degradation of ovine and caprine caseins. Food Chemistry 72:1 (2001), 79–88.
Vairo-Cavalli, S., Claver, S., Priolo, N., Natalucci, C., Extraction and partial characterization of a coagulant preparation from Silybum marianum flowers. Its action on bovine caseinate. Journal of Dairy Research 72:03 (2005), 271–275.
Van Vliet, T. (2000). Structure and rheology of gels formed by aggregated protein particles.
Veríssimo, P., Esteves, C., Faro, C., Pires, E., The vegetable rennet of Cynara cardunculus L. contains two proteinases with chymosin and pepsin-like specificities. Biotechnology Letters 17:6 (1995), 621–626.
Yu, L., Ngadi, M., Raghavan, G., Effect of temperature and pulsed electric field treatment on rennet coagulation properties of milk. Journal of Food Engineering 95:1 (2009), 115–118.
Zoon, P., Van Vliet, T., Walstra, P., Rheological properties of rennet-induced skim milk gels: 1. Introduction. Netherlands Milk and Dairy Journal 42 (1988), 249–269.
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