Surface-Enhanced Raman Scattering; Dopamine; Cellular analysis; Development of quantitative methods
Abstract :
[en] This communication reports the different steps undertaken in order to develop a new Surface-Enhanced Raman Scattering (SERS) nanosensor for the quantification of dopamine in the culture medium of PC-12 cells. First, the synthesis of the SERS substrate, gold nanoparticles, and its characterization. Then the development of the method with the help of a design of experiments and the demonstration of the specificity of the method over other structurally related catecholamines. And finally, the implementation of the method on the culture medium of PC-12 cells, on an HEPES buffer having served to wash the cells and on the PC-12 cells.
Research Center/Unit :
CIRM - Centre Interdisciplinaire de Recherche sur le Médicament - ULiège
Disciplines :
Chemistry Pharmacy, pharmacology & toxicology
Author, co-author :
Dumont, Elodie ; Université de Liège > Département de pharmacie > Chimie analytique
De Bleye, Charlotte ; Université de Liège > Département de pharmacie > Département de pharmacie
Cailletaud, Johan ; Université de Liège > Département de pharmacie > Chimie analytique
Jambo, Hugues ; Université de Liège > Département de pharmacie > Chimie analytique
Sacre, Pierre-Yves ; Université de Liège > Département de pharmacie > Chimie analytique
Hubert, Philippe ; Université de Liège > Département de pharmacie > Chimie analytique
Ziemons, Eric ; Université de Liège > Département de pharmacie > Département de pharmacie
Language :
English
Title :
Development of SERS nanosensors for the detection of small bioactive molecules by cellular analysis in complex matrices: Application to dopamine
Alternative titles :
[fr] Developpement de nanosenseurs SERS pour la détection par imagerie cellulaire de petites molécules bioactives dans des matrices complexes: Application à la dopamine
