Unpublished conference/Abstract (Scientific congresses and symposiums)
Evaluation of the Xpert®GBS LB test (Cepheid) performed on antenatal screening LIM enrichment broth for detection of Streptococcus agalactiae or group B streptococcus, compared to the reference culture method.
MEEX, Cécile; DUPONT, Audrey; SACHELI, Rosalie et al.
201720th Lancefield International Symposium on Streptococci and Streptococcal Diseases 2017
 

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Keywords :
Group B Streptococcus; Screening; Lim broth; Xpert GBS
Abstract :
[en] PCR, performed on LIM enrichment broth compared wit the reference method, that is subculture on selective differential agar performed from the same incubated Lim broth inoculated with a vagino-rectal swab collected at 35-37 weeks’ gestation. Material/methods: During an 8-months period in 2015-2016, series of consecutive vagino/rectal swabs collected for antenatal GBS screening (at the university hospital of Liege, Belgium) were plated first on Granada agar and then inoculated in selective enrichment LIM broth. The incubated broth was further sub-cultured on Granada and Biorad StrepBselect agars. Moreover, a sterile swab immersed in the same incubated broth was further analyzed by a real-time PCR targeting GBS using the Xpert®GBS LB test on the GeneXpert® system (Cepheid). Results: Among the 288 antenatal screenings included in the study, 48 (16.7%) were positive for GBS using the culture reference method and 51 Xpert®GBS LB test were positive (17.7%), includin the 48 samples positive in culture and 3 additional specimens for witch culture remained negative. Considering the enriched culture as the gold standard, the sensitivity and specificity of the Xpert®GBS LB test were 100% and 98.8% respectively. Conclusions: The Xpert®GBS LB test performed on incubated LIM broth is at least as efficient as selective enriched culture for antenatal screening of GBS. The turnaround-time and hands-on-ti are much shorter for the Xpert® GBS LB but it is more expensive than culture method, which may limit its use.
Research center :
CIRM - Centre Interdisciplinaire de Recherche sur le Médicament - ULiège
Disciplines :
Reproductive medicine (gynecology, andrology, obstetrics)
Pediatrics
Laboratory medicine & medical technology
Immunology & infectious disease
Author, co-author :
MEEX, Cécile ;  Centre Hospitalier Universitaire de Liège - CHU > Service de microbiologie clinique
DUPONT, Audrey
SACHELI, Rosalie  ;  Centre Hospitalier Universitaire de Liège - CHU > Service de microbiologie clinique
DESCY, Julie ;  Centre Hospitalier Universitaire de Liège - CHU > Service de microbiologie clinique
HUYNEN, Pascale ;  Centre Hospitalier Universitaire de Liège - CHU > Service de microbiologie clinique
HAYETTE, Marie-Pierre ;  Centre Hospitalier Universitaire de Liège - CHU > Service de microbiologie clinique
MELIN, Pierrette  ;  Centre Hospitalier Universitaire de Liège - CHU > Service de microbiologie clinique
Language :
English
Title :
Evaluation of the Xpert®GBS LB test (Cepheid) performed on antenatal screening LIM enrichment broth for detection of Streptococcus agalactiae or group B streptococcus, compared to the reference culture method.
Publication date :
19 October 2017
Event name :
20th Lancefield International Symposium on Streptococci and Streptococcal Diseases 2017
Event place :
Denarau Island, Fiji
Event date :
16th to 20th of October 2017
Audience :
International
Funders :
National Reference Centre Streptococcus agalactiae
Available on ORBi :
since 28 August 2017

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