[en] Malaria is a major public health problem in the Democratic Republic of Congo. Despite progress achieved over the past decade in the fight against malaria, further efforts have to be done such as in the surveillance and the containment of Plasmodium falciparum resistant strains. We investigated resistance to artemisinin-based combination therapies currently in use in Democratic Republic of Congo by surveying molecular polymorphisms in three genes: pfcrt, pfmdr1 and pfk13 to explore possible emergence of amodiaquine, lumefantrine or artemisinin resistance in Democratic Republic of Congo. This study essentially revealed that resistance to chloroquine is still decreasing while polymorphism related to amodiaquine resistance seems to be not present in Democratic Republic of Congo, that three samples, located in the east of the country, harbor Pfmdr1 amplification and that none of the mutations found in South-East Asia correlated with artemisinine resistance have been found in Democratic Republic of Congo. But new mutations have been identified, especially the M476K, occurred in the same position that the M476I previously identified in the F32-ART strain, strongly resistant to artemisinine. Antimalarial first-line treatments currently in use in Democratic Republic of Congo are not associated with emergence of molecular markers of resistance.
Disciplines :
Laboratory medicine & medical technology
Author, co-author :
Mvumbi Makaba, Dieudonné; University of Kinshasa > Department of basic sciences, school of medecine
Bobanga Lengu, Thierry
Kayembe Ntumba, Jean-Marie; Université de Kinshasa > Internal medecine
Mvumbi Lelo, Georges; Université de Kinshasa > Biochemistry and molecular biology unit
Situakibanza Nani-Tuma, Hippolyte; Université de Kinshasa > Internal medecine- Parasitology and tropical medecine
Benoit-Vical, Françoise; Centre National de la Recherche Scientifique - CNRS, Toulouse, France > Laboratoire de chimie de coordination
Melin, Pierrette ; Université de Liège > Département des sciences biomédicales et précliniques > Bactériologie, mycologie, parasitologie, virologie
De Mol, Patrick ; Université de Liège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Hayette, Marie-Pierre ; Université de Liège > Département des sciences biomédicales et précliniques > Bactériologie, mycologie, parasitologie, virologie
Language :
English
Title :
Molecular surveillance of Plasmodium falciparum resistance to artemisinin-based combination therapies in the Democratic Republic of Congo
Alternative titles :
[en] Surveillance moléculaire de la résistance de Plasmodium falciparum aux associations thérapeutiques à base d'artémisinine
Publication date :
08 June 2017
Journal title :
PLoS ONE
eISSN :
1932-6203
Publisher :
Public Library of Science, San Franscisco, United States - California
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
ARES - Académie de Recherche et d'Enseignement Supérieur
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