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Poster (Scientific congresses and symposiums)
IN VIVO STUDY OF THE SV2A PROTEIN IN THE KAINIC ACID EPILEPSY RAT MODEL
Serrano Navacerrada, Maria Elisa; Becker, Guillaume; Bahri, Mohamed Ali et al.
201712th European Molecular Imaging Meeting (EMIM 2017)
 

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Keywords :
SV2A; Kainic Acid Model; 18-UCB-H
Abstract :
[en] Introduction Epilepsy is one of the commonest neurological disorders [1]. Antiepileptic drugs mainly target the SV2A protein [2] but its actual role is still largely unknown. [18F]UCB-H was developed to study in vivo SV2A brain proteins [3, 4]. The present pilot study was undertaken to evaluate for the first time in vivo in rats SV2A expression in the Kaïnic Acid (KA) epilepsy model [5]. Although this model is well studied in mice, few reports were devoted to rats. Imaging-wise, rats are very interesting thanks to a bigger brain size (reduction of the partial volume effect). Methods Three male Sprague-Dawley were used, one injected with saline and two with multiple KA injections (3 x 5mg/kg) [6]. 75 days later, when spontaneous seizures started to appear, microPET (Focus 120 ) was performed under isoflurane anesthesia (2.5-3 % in air) for 1 hour with [18F]UCB-H (41 ± 5 MBq IV tail vein) followed by MRI (9.4T Agilent, anatomical T2). Coregistration was done with PMOD 3.6 software. Data were expressed as SUV and areas under the curve were calculated for the different regions. Results [18F]UCB-H microPET images showed an important reduction (20-30%) for SV2A after KA injections mainly localized in amygdala, hippocampus, lateral parietal association cortex and cingulate cortex. The rest of the brain was globally unchanged. MRI revealed atrophy and inflammation in amygdala and hippocampus. Conclusions These preliminary results obtained in KA treated rats showed that [18F]UCB-H was able to detect important modifications for SV2A in relevant regions for epilepsy and appears as a valuable tool to follow in vivo SV2A through longitudinal studies. KA model in rats deserves for further development and validation as a tool for the study of epilepsy.
Research Center/Unit :
GIGA CRC (Cyclotron Research Center) In vivo Imaging-Aging & Memory - ULiège
Disciplines :
Radiology, nuclear medicine & imaging
Author, co-author :
Serrano Navacerrada, Maria Elisa ;  Université de Liège > Centre de recherches du cyclotron
Becker, Guillaume ;  Université de Liège > Centre de recherches du cyclotron
Bahri, Mohamed Ali  ;  Université de Liège > Département de physique > Département de physique
Warnier, Corentin ;  Université de Liège - ULiège > Form. doct. sc. (chimie - Bologne)
Aerts, Joël ;  Université de Liège > Département de pharmacie > Département de pharmacie
Mievis, Frédéric ;  Université de Liège > Centre de recherches du cyclotron
Giacomelli, Fabrice ;  Université de Liège > Centre de recherches du cyclotron
Lemaire, Christian ;  Université de Liège > Centre de recherches du cyclotron
Salmon, Eric  ;  Université de Liège > Département des sciences cliniques > Neuroimagerie des troubles de la mémoire et révalid. cogn.
Luxen, André ;  Université de Liège > Département de chimie (sciences) > Laboratoire de chimie organique de synthèse
Plenevaux, Alain  ;  Université de Liège > Département de chimie (sciences) > Département de chimie (sciences)
Language :
English
Title :
IN VIVO STUDY OF THE SV2A PROTEIN IN THE KAINIC ACID EPILEPSY RAT MODEL
Publication date :
2017
Event name :
12th European Molecular Imaging Meeting (EMIM 2017)
Event organizer :
ESMI (European Society of Molecular Imaging)
Event place :
Cologne, Germany
Event date :
from 05-04-2017 to 07-04-2017
Audience :
International
Name of the research project :
ARC 13/17 - 07 (SYNAP - SV2A)
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique
Available on ORBi :
since 14 July 2017

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