Ex vivo expansion of hematopoietic progenitor cells is associated with downregulation of alpha4 integrin- and CXCR4-mediated engraftment in NOD/SCID beta2-microglobulin-null mice

Foguenne, Jacques; Di Stefano; Giet, Olivier; Beguin, Yves; Gothot, André

doi:10.3324/haematol.13206

Article (Scientific journals)

Ex vivo expansion of hematopoietic progenitor cells is associated with downregulation of alpha4 integrin- and CXCR4-mediated engraftment in NOD/SCID beta2-microglobulin-null mice

Foguenne, Jacques; Di Stefano; Giet, Olivier et al.

2009 • In Haematologica, 94, p. 185-194

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/21181

DOI
10.3324/haematol.13206

PubMed
19144663

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Keywords :

stem cells; hematopoiesis; cell trafficking

Abstract :

[en] Several studies indicate that ex vivo cytokine-supported expansion induces defective hematopoietic stem cell engraftment. We investigated the role of alpha4 integrin, alpha5 integrin and CXCR4 in engraftment of unmanipulated and cytokine-treated human cord blood CD34+ cells.

Disciplines :

Hematology

Author, co-author :

Foguenne, Jacques ; Centre Hospitalier Universitaire de Liège - CHU > Secteur assurance qualité

Di Stefano

Giet, Olivier ; Centre Hospitalier Universitaire de Liège - CHU > Thérapie cellulaire

Beguin, Yves ; Université de Liège - ULiège > Département des sciences cliniques > Hématologie - Oncologie médicale

Gothot, André ; Université de Liège - ULiège > Hématologie biologique et immuno hématologie

Language :

English

Title :

Ex vivo expansion of hematopoietic progenitor cells is associated with downregulation of alpha4 integrin- and CXCR4-mediated engraftment in NOD/SCID beta2-microglobulin-null mice

Publication date :

2009

Journal title :

Haematologica

ISSN :

0390-6078

eISSN :

1592-8721

Publisher :

Ferrata Storti Foundation, Pavia, Italy

Volume :

Pages :

185-194

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 07 September 2009

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Bibliography

Glimm H, Eaves CJ. Direct evidence for multiple self-renewal divisions of human in vivo repopulating hematopoietic cells in short-term culture. Blood 1999;94:2161-8.
Dao MA, Yu XJ, Nolta JA. Clonal diversity of primitive human hematopoietic progenitors following retroviral marking and long-term engraftment in immune-deficient mice. Exp Hematol 1997;25:1357-66.
Gothot A, van der Loo JCM, Clapp DW, Srour EF. Cell cycle-related changes in repopulating capacity of human mobilized peripheral blood CD34+ cells in NOD/SCID mice. Blood 1998;92:2641-9.
Habibian HK, Peters SO, Hsieh CC, Wuu J, Vergilis K, Grimaldi CI, et al. The fluctuating phenotype of the lymphohematopoietic stem cell with cell cycle transit. J Exp Med 1998;188:393-8.
Takatoku M, Sellers S, Agricola BA, Metzger ME, Kato I, Donahue RE, Dunbar CE. Avoidance of stimulation improves engraftment of cultured and retrovirally transduced hematopoietic cells in primates. J Clin Invest 2001;108:447-55.
Glimm H, Oh IH, Eaves CJ. Human hematopoietic stem cells stimulated to proliferate in vitro lose engraftment potential during their S/G2/M transit and do not reenter G0. Blood 2000;96:4185-93.
Szilvassy SJ, Bass MJ, Van Zant G, Grimes B. Organ-selective homing defines engraftment kinetics of murine hematopoietic stem cells and is compromised by ex vivo expansion. Blood 1999;93:1557-66.
Ahmed F, Ings SJ, Pizzey AR, Blundell MP, Thrasher AJ, Ye HT, et al. Impaired bone marrow homing of cytokine-activated CD34+ cells in the NOD/SCID model. Blood 2004;103:2079-87.
Dooner M, Cerny J, Colvin G, Demers D, Pimentel J, Greer D, et al. Homing and conversion of murine hematopoietic stem cells to lung. Blood Cells Mol Dis 2004;32:47-51.
Giet O, Huygen S, Beguin Y, Gothot A. Cell cycle activation of hematopoietic progenitor cells increases very late antigen-5-mediated adhesion to fibronectin. Exp Hematol 2001;29:515-24.
Giet O, Van Bockstaele DR, Di Stefano I, Huygen S, Greimers R, Beguin Y, Gothot A. Increased binding and defective migration across fibronectin of cycling hematopoietic progenitor cells. Blood 2002;99:2023-31.
Huygen S, Giet O, Artisien V, Di Stefano I, Beguin Y, Gothot A. Adhesion of synchronized human hematopoietic progenitor cells to fibronectin and vascular cell adhesion molecule-1 fluctuates reversibly during cell cycle transit in ex vivo culture. Blood 2002;100:2744-52.
Foguenne J, Huygen S, Greimers R, Beguin Y, Gothot A. Modulation of homing properties of primitive progenitor cells generated by ex vivo expansion. Haematologica 2005;90:445-51.
Papayannopoulou T, Craddock C, Nakamoto B, Priestley GV, Wolf NS. The VLA4/VCAM-1 adhesion pathway defines contrasting mechanisms of lodgement of transplanted murine hemopoietic progenitors between bone marrow and spleen. Proc Natl Acad Sci USA 1995;92:9647-51.
Craddock CF, Nakamoto B, Elices M, Papayannopoulou T. The role of CS1 moiety of fibronectin in VLA mediated haemopoietic progenitor trafficking. Br J Haematol 1997;97:15-21.
van der Loo JC, Xiao X, McMillin D, Hashino K, Kato I, Williams DA. VLA-5 is expressed by mouse and human long-term repopulating hematopoietic cells and mediates adhesion to extracellular matrix protein fibronectin. J Clin Invest 1998;102:1051-61.
Papayannopoulou T, Priestley GV, Nakamoto B, Zafiropoulos V, Scott LM, Harlan JM. Synergistic mobilization of hemopoietic progenitor cells using concurrent beta1 and beta2 integrin blockade or beta2-deficient mice. Blood 2001;97:1282-8.
Peled A, Kollet O, Ponomaryov T, Petit I, Franitza S, Grabovsky V, et al. The chemokine SDF-1 activates the integrins LFA-1, VLA-4, and VLA-5 on immature human CD34+ cells: role in transendothelial/stromal migration and engraftment of NOD/SCID mice. Blood 2000;95:3289-96.
Yahata T, Ando K, Sato T, Miyatake H, Nakamura Y, Muguruma Y, et al. A highly sensitive strategy for SCID-repopulating cell assay by direct injection of primitive human hematopoietic cells into NOD/SCID mice bone marrow. Blood 2003;101:2905-13.
Lataillade JJ, Clay D, Bourin P, Herodin F, Dupuy C, Jasmin C, Bousse-Kerdiles MC. Stromal cell-derived factor 1 regulates primitive hematopoiesis by suppressing apoptosis and by promoting G0/G1 transition in CD34+ cells: evidence for an autocrine/paracrine mechanism. Blood 2002;99:1117-29.
Lataillade JJ, Clay D, Dupuy C, Rigal S, Jasmin C, Bourin P, Bousse-Kerdiles MC. Chemokine SDF-1 enhances circulating CD34+ cell proliferation in synergy with cytokines: possible role in progenitor survival. Blood 2000;95:756-68.
Cashman J, Clark-Lewis I, Eaves A, Eaves C. Stromal-derived factor 1 inhibits the cycling of very primitive human hematopoietic cells in vitro and in NOD/SCID mice. Blood 2002;99:792-9.
Kim CH, Broxmeyer HE. In vitro behavior of hematopoietic progenitor cells under the influence of chemoattractants: stromal cell-derived factor-1, steel factor, and the bone marrow environment. Blood 1998;91:100-10.
Chiu CP, Dragowska W, Kim NW, Vaziri H, Yui J, Thomas TE, et al. Differential expression of telomerase activity in hematopoietic progenitors from adult human bone marrow. Stem Cells 1996;14:239-48.
Glimm H, Eisterer W, Lee K, Cashman J, Holyoake TL, Nicolini F, et al. Previously undetected human hematopoietic cell populations with short-term repopulating activity selectively engraft NOD/SCID-beta2 microglobulin-null mice. J Clin Invest 2001;107:199-206.
Lemoine FM, Humphries RK, Abraham SD, Krystal G, Eaves CJ. Partial characterization of a novel stromal cell-derived pre-B-cell growth factor active on normal and immortalized pre-B cells. Exp Hematol 1988;16:718-26.
Taswell C. Limiting dilution assays for the determination of immunocompetent cell frequencies. J Immunol 1981;126:1614-9.
Rose DM, Cardarelli PM, Cobb RR, Ginsberg MH. Soluble VCAM-1 binding to alpha 4 integrins is cell-type specific and activation dependent and is disrupted during apoptosis in T cells. Blood 2000;95:602-9.
Luque A, Gomez M, Puzon W, Takada Y, Sanchez-Madrid F, Cabanas C. Activated conformations of very late activation integrins detected by a group of antibodies (HUTS) specific for a novel regulatory region (355-425) of the common beta 1 chain. J Biol Chem 1996;271:11067-75.
Arroyo AG, Sanchez-Mateos P, Campanero MR, Martin-Padura I, Dejana E, Sanchez-Madrid F. Regulation of the VLA integrin-ligand interactions through the beta 1 subunit. J Cell Biol 1992;117:659-70.
Miyake K, Weissman IL, Greenberger JS, Kincade PW. Evidence for a role of the integrin VLA-4 in lympho-hemopoiesis. J Exp Med 1991;173:599-607.
Ramirez M, Segovia JC, Benet I, Arbona C, Guenechea G, Blaya C, et al. Ex vivo expansion of umbilical cord blood (UCB) CD34+ cells alters the expression and function of alpha 4 beta 1 and alpha 5 beta 1 integrins. Br J Haematol 2001;115:213-21.
Chute JP, Saini AA, Kampen RL, Wells MR, Davis TA. A comparative study of the cell cycle status and primitive cell adhesion molecule profile of human CD34+ cells cultured in stroma-free versus porcine microvascular endothelial cell cultures. Exp Hematol 1999;27:370-9.
Reems JA, Mielcarek M, Torok-Storb B. Differential modulation of adhesion markers with ex vivo expansion of human umbilical CD34+ progenitor cells. Biol Blood Marrow Transplant 1997;3:133-41.
Springer TA. Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm. Cell 1994;76:301-14.
Chan JR, Cybulsky MI. Detection of high-affinity alpha4-integrin upon leukocyte stimulation by chemoattractants or chemokines. Methods Mol Biol 2004;239:261-8.
Garcia-Gila M, Cabanas C, Garcia-Pardo A. Analysis of the activation state of alpha4beta1 integrin in human B cell lines derived from myeloma, leukemia or lymphoma. FEBS Lett 1997;418:337-40.
Grabovsky V, Feigelson S, Chen C, Bleijs DA, Peled A, Cinamon G, et al. Subsecond induction of alpha4 integrin clustering by immobilized chemokines stimulates leukocyte tethering and rolling on endothelial vascular cell adhesion molecule 1 under flow conditions. J Exp Med 2000;192:495-506.
Peled A, Petit I, Kollet O, Magid M, Ponomaryov T, Byk T, et al. Dependence of human stem cell engraftment and repopulation of NOD/SCID mice on CXCR4. Science 1999;283:845-8.
Frenette PS, Subbarao S, Mazo IB, von Andrian UH, Wagner DD. Endothelial selectins and vascular cell adhesion molecule-1 promote hematopoietic progenitor homing to bone marrow. Proc Natl Acad Sci USA 1998;95:14423-8.
Priestley GV, Scott LM, Ulyanova T, Papayannopoulou T. Lack of α4 integrin expression in stem cells restricts competitive function and self-renewal activity. Blood 2006;107:2959-67.
Kovach NL, Carlos TM, Yee E, Harlan JM. A monoclonal antibody to beta 1 integrin (CD29) stimulates VLA-dependent adherence of leukocytes to human umbilical vein endothelial cells and matrix components. J Cell Biol 1992;116:499-509.
van Kooyk Y, Figdor CG. Avidity regulation of integrins: the driving force in leukocyte adhesion. Curr Opin Cell Biol 2000;12:542-7.
Peled A, Grabovsky V, Habler L, Sandbank J, Arenzana-Seisdedos F, Petit I, et al. The chemokine SDF-1 stimulates integrin-mediated arrest of CD34+ cells on vascular endothelium under shear flow. J Clin Invest 1999;104:1199-211.
Cerny J, Dooner M, McAuliffe C, Habibian H, Stencil K, Berrios V, et al. Homing of purified murine lymphohematopoietic stem cells: a cytokine-induced defect. J Hematother Stem Cell Res 2002;11: 913-22.