Reference : Validation of a Multiplex HPLC MS/MS assay for the measurement of steroid hormones
Dissertations and theses : Master's dissertation
Human health sciences : Laboratory medicine & medical technology
Validation of a Multiplex HPLC MS/MS assay for the measurement of steroid hormones
Rademaker, Gilles mailto [Université de Liège > Département des sciences biomédicales et précliniques > GIGA-R : Labo de recherche sur les métastases >]
Université de Liège, ​Liège, ​​Belgique
Master en sciences pharmaceutiques, à finalité approfondie
CAVALIER, Etienne mailto
[en] Steroids ; Mass spectrometry ; Validation
[en] Facing an improvement of the quality assurance in all the laboratory, the validation of methods has become a step that cannot be got round. The objective of this work is the validation of a Protein-Precipitation HPLC-MS/MS method for the measurement of steroid hormones (Aldosterone, Androstenedione, Cortisol, 17-OH Progesterone, DHEAS and Testosterone). To achieve this purpose, a precision, recovery, linearity, carry over assay and a limit of quantification are performed. After collection of the data, results are calculated and statistically analysed. They allow to set boundaries to the method and to understand more easily how we could improve it. The results show that the method is effective for all hormones except the aldosterone, for which the method still has to be improved. Moreover, a comparison of the analysis of the testosterone between Cobas and HPLC MS/MS is made. In the lab, this hormone is analysed with Cobas but a transition to the HPLC MS/MS can be done because the results are satisfactory.
Validation of a multiplex HPLC-MS/MS assay for the measurement of steroid hormones
Researchers ; Professionals

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