Presence of γ-aminobutyric acid transporter mRNA in interneurons and principal cells of rat hippocampus

[en] After release, neurotransmitters are removed from the extracellular space by high-af®nity uptake. Speci®c sodiumdependent transporters serve this function for the inhibitory transmitter g-aminobutyric acid (GABA). However, it is largely unknown to which proportion GABA is taken up by GABAergic interneurons, glia cells or principal neurons. We analyzed the distribution of mRNA for the main GABA-transporter subtype in the hippocampus, GAT-1, in adult rats. Most interneurons were strongly stained for GAT-1 mRNA, indicating re-uptake by the GABA-releasing cells. Surprisingly, prominent signals for GAT-1 were also found throughout the principal cell layers (granule and pyramidal cells). These data indicate that GABA transporters may be present in non-GABAergic projection cells of the rat hippocampus which contribute to the clearance of GABA from the extracellular space

Disciplines :

Anatomy (cytology, histology, embryology...) & physiology

Author, co-author :

Frahm, Christiane

Engel, Dominique ; Université de Liège > Département des sciences biomédicales et précliniques > Pharmacologie

Piechotta, Antje

Heinemann, Uwe

Draguhn, Andreas

Language :

English

Title :

Presence of γ-aminobutyric acid transporter mRNA in interneurons and principal cells of rat hippocampus

Publication date :

2000

Journal title :

Neuroscience Letters

ISSN :

0304-3940

eISSN :

1872-7972

Publisher :

Elsevier, Limerick, Ireland

Volume :

288

Issue :

Pages :

175-178

Peer reviewed :

Peer Reviewed verified by ORBi

Name of the research project :

SFB 515/B1

Funders :

DFG - Deutsche Forschungsgemeinschaft

Available on ORBi :

since 31 May 2017

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